Fig. 3

MaMYBA1, MaMYBA2 and MaMYBPA2 can activate proAtDFR and proAtANS as part of an MBW complex. The ability of MaMYBs to activate proAtDFR-GUS and proAtANS-GUS reporter constructs in combination with different bHLH proteins (AtEGL3, ZmR) and a WDR (AtTTG1) was analysed by co-transfection in A. thaliana At7 protoplasts. The relative promoter activity refers to the measured GUS reporter enzyme activity. Promoter activity is given relative to the values obtained for the A. thaliana MBW complex (AtPAP1, AtEGL3, AtTTG1). Error bars indicate the standard deviation of five independent biological replicates. Statistical significance is indicated by asterisks which mark p-values < 0.05.