Fig. 3

CITED1 overexpression alters expression of amphiregulin. A Characterization of CITED1 (28 kDa) and ERα (66KDa) protein expression in stable CITED1-overexpressing MCF7 cells compared to the empty vector (EV) control following passage (P) under selection (G418 antibiotic). β-tubulin (55 kDa) is used as a loading control. B CITED1 concentration, relative to a IPO8 control, in stable CITED1-overexpressing MCF7 cells (orange) compared to the empty vector control (blue) in response to estrogen stimulation (E2), tamoxifen (TAM) or simultaneous (E2/TAM) treatment. C, D Concentration of AREG and TGFα relative to a IPO8 control is shown in stable CITED1-overexpressing MCF7 cells (orange) compared to the stable empty vector control (blue). The response to estrogen stimulation (E2), tamoxifen (TAM) or simultaneous (E2/TAM) treatment is shown. E AREG protein expression in stable CITED1-overexpressing MCF7 cells compared to the empty vector (EV) control following passage under selection. The most intense band signal for AREG was just over the 25KDa marker which would likely correspond to the previously reported 26-28KDa cell surface form [30]. Total protein staining is used as a loading control. F AREG protein expression in stable CITED1-overexpressing MCF7 cells compared to the empty vector (EV) control in response to estrogen stimulation (E2), tamoxifen (TAM) or simultaneous (E2/TAM) treatment. Total protein staining is used as a loading control