Table 1 GeneXpert Ultra, phenotypic drug-susceptibility and rpoB sequencing results for 28 selected Mycobacterium tuberculosis isolates
From: GeneXpert MTB/RIF Ultra performance to detect uncommon rpoB mutations in Mycobacterium tuberculosis
Culture number | rpoB mutation(s) | Type of mutation | Appearance | WHO catalogue V1 | pDST | GeneXpert Ultra (V3) result | |||
|---|---|---|---|---|---|---|---|---|---|
Final confidence grading | Presence among (SOLO)RR isolates | LJ | MGIT | Report | Per probe analysis based on Tm for combo mutants | ||||
CT2005-00009 | Ins431(agCCAc); P439L | INDEL-no fs | Combo | No data; Ass w R-int | No data; 0 | RMP-R | NT | No R detected | No R detected; No R detected |
CT2008-02955 | Del427-428(ACCAGC)a | INDEL-no fs | Single | Not ass w Rb | No data | RMP-Se | RMP-Sd | Indeterminate | |
CT2005-02316 | Del437(AAC) | INDEL-no fs | Single | Ass w R-intb | No data | RMP-R | RMP-R | R detected | |
CT1993-09583 | H445R; N487Ha | SNP | Combo | Ass w R; uncert sign | 79; 2 | RMP-R | RMP-R | R detected | R detected; not applicable |
CT2007-00288 | D435Y; P454L | SNP | Combo | Ass w R; uncert sign | 44; 6 | RMP-R | RMP-Sd | R detected | R detected; R detected? |
CT1998-00166 | S428R; H445R | SNP | Combo | Ass w R-int; Ass w R | 5; 79 | RMP-R | R detected | R detected; R detected | |
CT2014-02522 | Q429L; D435V | SNP | Combo | Ass w R-int; Ass w R | 9; 732 | RMP-R | R detected | R detected; R detected | |
CT2004-01679 | S431G; S450W | SNP | Combo | Ass w R-int; Ass w R | 6; 151 | RMP-R | R detected | R detected; R detected | |
CT1997-01244 | M434I; D435Y | SNP | Combo | Ass w R-int; Ass w R | 16; 162 | RMP-R | R detected | R detected; R detected | |
CT2014-00803 | N437S; R447P | SNP | Combo | Ass w R-int; no data | 2; no data | RMP-R | R detected | R detected; R detected | |
CT2009-01036 | M434T; H445D | SNP | Combo | Ass w R-intb; Ass w R | No data; 268 | Indeterminate | Indeterminate; R detected | ||
CT2014-00781 | D435N; S450V | SNP | Combo | Ass w R-intb; Ass w R-int | No data; 2 | RMP-R | R detected | R detected; R detected | |
CT2012-00752 | Q432L | SNP | Single | Ass w R | 21 | RMP-R | Indeterminate | ||
CT2014-02541 | Q432L | SNP | Single | Ass w R | 21 | RMP-R | Indeterminate | ||
CT2008-01914 | Q432K | SNP | Single | Ass w R | 34 | RMP-R | R detected | ||
CT2014-01261 | Q432K | SNP | Single | Ass w R | 34 | RMP-R | R detected | ||
CT2013-01926 | Q432P | SNP | Single | Ass w R | 29 | RMP-R | NT | Indeterminate | |
CT2015-00488 | Q432P | SNP | Single | Ass w R | 29 | RMP-R | Indeterminate | ||
CT2018-03321 | S428R | SNP | Single | Ass w R-int | 5 | RMP-Sc | RMP-Sd | R detected | |
CT2019-04011 | Q432E | SNP | Single | Ass w R-int | 1 | RMP-R | R detected | ||
CT2014-00323 | D435A | SNP | Single | Ass w R-int | 2 | RMP-Sc | RMP-Sd | R detected | |
CT2015-00705 | D435A | SNP | Single | Ass w R-int | 2 | RMP-Sc | RMP-Sd | R detected | |
CT1997-01460 | H445P | SNP | Single | Ass w R-int | 5 | RMP-R | RMP-R | R detected | |
CT2008-01398 | H445P | SNP | Single | Ass w R-int | 5 | RMP-R | R detected | ||
CT2014-00787 | K446Q | SNP | Single | Ass w R-int | 7 | RMP-R | Indeterminate | ||
CT2014-00789 | K446Q | SNP | Single | Ass w R-int | 7 | RMP-R | Indeterminate | ||
CT2014-00761 | N438K | SNP | Single | Ass w R-intb | No data | RMP-R | R detected | ||
CT2013-00428 | G442R | SNP | Single | Ass w R-intb | No data | RMP-R | R detected | ||