Fig. 1
Amplified DNA fragments using the designed primer set. M = Generuler 100 bp, (Thermofisher Scientific, Waltham, MA, USA); B = Buffer control. Strain specific bands were generated using either gDNA (most left lane of every strain) or 3 individual single colonies of the respective strain. Expected fragment sizes: TMW 2.2254 = 2552 bp; TMW 2.2256 = 596 bp; TMW 2.2257 = 822 bp; TMW 2.2260 = 1119 bp; TMW 2.2263 = 333 bp; TMW 2.2264 = 1583 bp; TMW 2.2266 = 1961 bp; DSM 20339T = 2882 bp. The picture was taken with a Gel Jet-imager system (Intas Science Imaging, Instruments GmbH, Göttingen, Germany) with the device software version 3.2.3.4089