Table 1 Enzyme concentration and incubation times evaluated for the protoplast isolation from mesophyll in Ricinus communis
Treatment | Enzymatic concentration (% w/v) | Incubation time (h) |
|---|---|---|
T1 | Cellulase 1% | 8, 10, 12, 14, 16, 18 |
Pectinase 1% | ||
Hemicellulase 0.15% | ||
T2 | Cellulase 2% | 8, 10, 12, 14, 16, 18 |
Pectinase 2% | ||
Hemicellulase 0.3% | ||
T3 | Cellulase 1.5% | 8, 10, 12, 14, 16, 18 |
Peptinase Rhizopus sp. 0.4% | ||
T4 | Cellulase “Onozuka” R-10 1.6% | 14, 16, 18 |
Macerozyme R-10 0.8% |