Table 1 Library preparation and sequencing summary metrics
From: Evaluation of rRNA depletion methods for capturing the RNA virome from environmental surfaces
Sample | Spiked synthetic RNA copies per library | rRNA depletion | Average library size (bp) | Library molarity (nM) | No. of paired reads | Passed Filter (%) | Duplication rate (%) | GC content | % Eukaryotic rRNA reads (18S, 28S) | % Bacterial rRNA reads (16S, 23S) | % Non-ribosomal RNA reads |
|---|---|---|---|---|---|---|---|---|---|---|---|
TH | NA | Human | 335 | 45.4 | 9,431,639 | 96.4 | 55.4 | 52.8 | 4.3 | 85.8 | 9.9 |
TH-s100k | 105 | Human | 331 | 43.1 | 11,565,081 | 97.7 | 59.8 | 52.7 | 4.5 | 86.9 | 8.6 |
TB | NA | Bacteria | 329 | 48.0 | 10,890,472 | 96.5 | 57.9 | 52.3 | 89.2 | 1.1 | 9.6 |
TB-s100k | 105 | Bacteria | 329 | 61.3 | 9,456,268 | 97.0 | 57.2 | 52.2 | 88.4 | 0.8 | 10.8 |
TB-s10k | 104 | Bacteria | 330 | 43.1 | 10,113,548 | 97.0 | 59.3 | 52.3 | 88.0 | 1.2 | 10.8 |
TB-s1k | 103 | Bacteria | 331 | 63.7 | 10,001,537 | 97.5 | 56.6 | 52.2 | 87.8 | 0.8 | 11.3 |
TB-s0.1 k | 102 | Bacteria | 337 | 61.3 | 9,888,461 | 97.3 | 55.1 | 52.3 | 88.9 | 0.9 | 10.2 |
TB-s0.01 k | 101 | Bacteria | 325 | 43.8 | 10,581,258 | 97.5 | 61.9 | 52.4 | 89.0 | 0.8 | 10.2 |
NH | NA | Human | 276 | 2.2 | 5,429,140 | 53.0 | 63.4 | 50.9 | 11.6 | 28.5 | 59.0 |
NB | NA | Bacteria | 309 | 2.8 | 6,926,797 | 85.4 | 75.1 | 52.4 | 23.9 | 29.2 | 46.4 |