Fig. 1

MOPS gel electrophoresis (A) and Bioanalyzer (B) showing the integrity of extracted RNA using a modified Spin column kit. The estimated sizes of 23 S and 16 S ribosomal subunits is 3000 bp and 1500 bp, respectively. Lanes in Fig. A: Lane 2–4 (KZN B1, B2 and B3), 5–7 (Beijing B1, B2 and B3), 8–10 (F11 B1, B2 and B3), 11–13 (H37Rv B1, B2 and B3), 14–16 (H37Rv Chol B1, Chol B2 and Chol B3); which correspond to Lane A1 to Lane G1, respectively in the Bioanalyzer image (shown in details on Table 2). (C) A 1.2% agarose gel showing PCR product for ASdes (Lane 1), ASpks (Lane 2), MTS2823 (Lane 3) and Mcr11 (Lane 4) for the expected product sizes of 195, 80, 300 and 100 bp, respectively, using the cDNA synthesized from the H37Rv laboratory strain cultured in minimal media supplemented with cholesterol. The agarose gel electrophoresis was running at 80 V for 50 min