Table 1 sRNA primers that were used for PCR for H37Rv strain grown in cholesterol-rich minimal media
sRNA | Forward Primer (5’-3’) | Reverse Primer (5’-3’) | Expected product size (bp) | Annealing temperatures (°C) |
|---|---|---|---|---|
ASdes | CATAGAGGACGGAGTCGG | AGTGGGTCAACCGTT | 195 | 51 |
ASpks | GGCCTGCGTATGACCCATATT | CTTATGGGCAAGATCGGGGG | 80 | 57 |
MTS2823 | TAGTACAAAGGAACCAC | GACCCGAAGAACTCGA | 300 | 55 |
Mcr11 | AAAGTGCCGGAAGACGGG | CGGTACACATGGGCAGACCC | 100 | 52 |