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Table 2 Primers used and PCR amplicons sizes

From: Expression of hes, iha, and tpsA codified in locus of adhesion and autoaggregation and their involvement in the capability of shiga toxin-producing Escherichia coli strains to adhere to epithelial cells

Primer

Sequence (5´-3’)

Size (bp)

Reference

hes_for

AGGTCATCACGCCAGTAACC

113

This study

hes_rev

CAGTTCAGTATTCCGGTTCG

  

iha_for

TTTCAGCCAGCAGCATGGCA

172

[9]

iha_rev

ACATCCACACCCTCCACAGC

  

tpsA_for

CACCCGTACCGTGGAAGAAACC

174

[9]

tpsA_rev

TCGCCACTGACACTGACATTTTCC