Fig. 2

The expression of ZO-1 protein was evaluated by IHC in colon samples. WCF213 significantly maintained ZO-1 expression of tight junction (p = 0.000) (a). Moreover, ZO-1 protein expression persisted in normal and WCF213 + 2.5% DSS groups (b, d) as compared with the DSS-only group (c) (violet arrow: ZO-1 expressed in brown colour on the cellular membrane; black arrow: lack of brown colour as lack of ZO-1 expressed on the cellular membrane). Data expressed as mean ± SEM (n = 4). (^##p < 0.001 vs. DSS group). IHC results were observed at 400-fold magnification. All observation was performed in triplicate