Fig. 3

Performance of the duplex LAMP assay. a Sensitivity. Duplex LAMP assay was performed for tenfold serial dilutions (100 ng–1 pg) of the male and female DNA samples. b Mixed DNA samples. Duplex LAMP assay was performed for mixed DNA samples with male:female ratios of 1:1 to 1:10,000 (male DNA = 10 pg). The samples with ratios of 1:0 and 0:1 were the male DNA (10 pg) and female DNA (100 ng), respectively. a, b Data are expressed as mean ± SD (n = 3). Data at a threshold time of 60 min represent samples that were not detected