Fig. 3From: A hybrid RNA FISH immunofluorescence protocol on Drosophila polytene chromosomesRNA FISH on wildtype D. melanogaster polytene chromosomes with a single copy histone array transgene (WT + 1 HA) using probes against A mini-white (mw; green), B roX2 (green) in males, and C roX2 (green) in females. Histone2b (h2b) RNA FISH (green) on wildtype polytenes (WT; 100 histone arrays) D without RNase treatment and E with RNase treatment. DNA is stained with DAPI (red)Back to article page