Table 1 A panel of strains used for the assay specificity validation
No. | Isolate/strain* | Ct | Parental strains | Sample | Ct** (25) | Phylogenetic cluster | Accession number of full genome |
|---|---|---|---|---|---|---|---|
1 | Dagestan/2015 | Neg | Not applicable | Cell culture | 15.10 | 1.2 | MH893760 |
2 | Ethiopia/1995 | Neg | Not applicable | Cell culture | 23.5 | 1.2 | Not available |
3 | Saratov/2017 | Neg | Neethling-major, KSGP-minor | Cell culture | 22.30 | 2.1 | MH646674 |
4 | Kurgan/2018 | 14.89 | KSGP-major, Neethling-minor | Skin scabs | 13.75 | 2.6 | OP948721 |
5 | Chelyabinsk/2018 | Neg | Neethling-major, KSGP-minor | Skin scabs | 16.91 | Not determined | Not available |
6 | Samara/2018 | 21.68 | KSGP-major, Neethling-minor | Skin scabs | 20.89 | Not determined | Not available |
7 | Udmurtiya/2019 | 22.23 | KSGP-major, Neethling-minor | Cell culture | 22.14 | 2.2 | MT134042 |
8 | Saratov/2019 | Neg | Neethling-major, KSGP-minor | Cell culture | 25.42 | 2.1 | OM530217 |
9 | Tyumen/2019 | Neg | Neethling-major, KSGP-minor | Cell culture | 23.17 | 2.4 | OL542833 |
| Â | Khabarovsk/2020 | Neg | Neethling-major, KSGP-minor | Cell culture | 19.87 | 2.5 | OM793603 |
10 | EAO/2020 | Neg | Neethling-major, KSGP-minor | Nasal swab | 16.77 | 2.5 | Not available |
11 | Altay/2020 | Neg | Neethling-major, KSGP-minor | Cell culture | 23.65 | 2.5 | OP948720 |
12 | Tomsk/2020 | Neg | Neethling-major, KSGP-minor | Cell culture | 24.41 | 2.5 | OM793602 |
13 | Mongolia/2021 | Neg | Neethling-major, KSGP-minor | Skin scab | 18.50 | 2.5 | Not available |
14 | Buryatiya/2021 | Neg | Neethling-major, KSGP-minor | Cell culture | 20.16 | 2.5 | OP948726 |
15 | Zabaykalsky/2021 | Neg | Neethling-major, KSGP-minor | Cell culture | 26.20 | 2.5 | OP948719 |
16 | Amur/2022 | Neg | Neethling-major, KSGP-minor | Cell culture | 26.31 | 2.5 | Not available |
17 | Buryatiya/2021 | Neg | Neethling-major, KSGP-minor | Cell culture | 20.16 | 2.5 | OP948726 |
18 | Tuva/2022 | Neg | Neethling-major, KSGP-minor | Cell culture | 19.25 | 2.5 | Not available |
19 | KSGPO-240/Kenya/1959 | 25.17 | Not applicable | DNA | 24.90 | 1.2-KSGPO | KX683219 |
20 | Lumpivax vaccine | 27.41 | Not applicable | DNA | 27.62 | 1.2-KSGPO | Not available |
21 | Sverdlovsk/2018 | 31.01 | Not determined | Nasal swab | 31.22 | Not determined | Not available |
22 | Omsk/2018 | 29.00 | Not determined | Nasal swab | 28.64 | Not determined | Not available |
23 | Afghanistan | Neg | Not applicable | Cell culture | Neg | Sheep pox | Not available |
24 | NISKHI | Neg | Not applicable | Cell culture | Neg | Sheep pox | AY077834 |
25 | Moscow/2018 | Neg | Not applicable | Cell culture | Neg | Sheep pox | ON961656 |
26 | Dagestan/2020 | Neg | Not applicable | Skin scabs | Neg | Sheep pox | OQ434236 |
27 | Amur/2018 | Neg | Not applicable | Skin scabs | Neg | Sheep pox | OQ434235 |