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Table 2 Repeatability of the assay in one run (5 repetitions) and across 3 runs (15 repetitions)

From: Development and application of a real-time polymerase chain reaction assay to detect lumpy skin disease virus belonging to the Kenyan sheep and goat pox group

PCR

Mean Ct

SD

СV (%)

Run 1

29.68

0.97

3.27

Run 2

30.26

1.1

3.64

Run 3

29.93

0.56

1.87

n = 15

Across runs

29.96

0.88

2.94

  1. PCR: polymerase chain reaction; Ct: cycle threshold; SD: standard deviation; CV: coefficient of variation
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BMC Research Notes

ISSN: 1756-0500

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