Table 1 TaqMan primers and probes sequence for the human MMPs and GAPDH
From: The expression of matrix metalloproteinase 2, 9 and 11 in Ethiopian breast cancer patients
Gene | Gene bank accession no. | Â | The sequence of primers and FAM-BHQ1 probes | Amplicon size (bp) |
|---|---|---|---|---|
MMP-2 | NM004530 | Forward primer (5’-3’) | TGGCGATGGATACCCCTTT | 118 |
Reverse primer (5’-3’) | TTCTCCCAAGGTCCATAGCTCAT | |||
Probe (5’-3’) | FAMCTCCTGGCTCATGCCTTCGCCCBHQ1 | |||
MMP-9 | NM004994 | Forward primer (5’-3’) | CCTGGGCAGATTCCAAACCT | 89 |
Reverse primer (5’-3’) | GCAAGTCTTCCGAGTAGTTTTGGAT | |||
Probe (5’-3’) | FAMCTCAAGTGGCACCACCACAACATCACCBHQ1 | |||
MMP-11 | NM005940 | Forward primer (5’-3’) | CCGCCAGATGCCTGTGA | 92 |
Reverse primer (5’-3’) | CGGAGGCGCCACACAA | |||
Probe (5’-3’) | FAMCCTCCTTTGACGCGGTCTCCACCBHQ1 | |||
GAPDH | NM001357943 | Forward primer (5’-3’) | GAAGGTGAAGGTCGGAGTC | 226 |
Reverse primer (5’-3’) | GAAGATGGTGATGGGATTTC | |||
Probe (5’-3’) | FAMCAAGCTTCCCGTTCTCAGCCBHQ1 |