Fig. 2
Bioenergetic profile of coupling assays in isolated mitochondria. (a) Determination of optimal mitochondrial input per well (1000, 500, 250 125 ng) in the presence of succinate/rotenone. (b) Absolute O2 tension (in mm Hg) in microchamber with different mitochondrial inputs. (c) Coupled mitochondrial respiration assays using 10 mM pyruvate/2 mm malate (PM), 10 mM glutamate/10 mM malate (GM), 40 μm palmitoyl L-carnitine/1 mM malate (PCM) and 10 mM succinate/2 uM rotenone (SR) as substrates with 600 ng mitochondrial input per well. (d) OCR for States 2, 3 4o and 3u and RCR for each of the substrates in (c). For Figures (a-c) data are expressed as pmol/min ± SEM or mm Hg ± SEM and in (d), as pmol/min ± SEM or the ratio of State 3/State 4o (RCR).