Fig. 1

Investigation of the binding ability of galectin-2 to S. mansoni glycoconjugates. (A)S. mansoni extracts were subjected to SDS-PAGE and lectin blotting using HRP-labelled galectin-2. (B, C)S. mansoni protein bound by galectin-2 was isolated by affinity chromatography using an immobilized galectin-2 column and eluted with lactose. It was subjected to SDS-PAGE and (A) CBB-staining or (B) Pro-Q emerald staining. The arrows in B and C indicate galectin-2-binding glycoprotein identified by mass spectrometry. (D) Adult pairs of S. mansoni were stained with Alexa488-labeled galectin-2 or treated with PBS (negative control). Arrows and arrowheads indicate galectin-2-dependent staining of the worms