Table 1 Primers used in this study for the detection of the different carbapenemase genes
Genes | Size (bp) | Annealing (°C) | Nucleotide Sequences (5’-3’) |
|---|---|---|---|
Bla VIM | 390 | Â 54 | F: GATGGTGTTTGGTCGCATA/R: CGAATGCGCAGCACCAG |
bla NDM | 621 | Â 57 | F: GGTTTGGCGATCTGGTTTTC/R: CGGAATGGCTCATCACGATC |
bla KPC | 893 | Â 55 | F: ATGTCACTGTATCGCCGTCT/R: TTTTCAGAGCCTTACTGCCC |
bla OXA−48−like | 744 | 58 | F: TTGGTGGCATCGATTATCGG/R: GAGCACTTCTTTTGTGATGGC |
bla IMP | 740 | 49 | F: TGAGCAAGTTATCTGTATTC/R: TTAGTTGCTTGGTTTTGATG |