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Fig. 1 | BMC Research Notes

Fig. 1

From: Expression of dengue virus and Zika virus NS2B-NS3pro constructs alter cellular fatty acids, but co-expression with a Zika virus virus-like particle is detrimental to virus-like particle expression

Fig. 1

Expression of NS2B-NS3pro and NS2B-NS3pro (S135A) constructs in HEK293T/17 cells. (A) ZIKV NS2B-NS3pro and NS2B-NS3(S135A) and (B) DENV NS2B-NS3pro and NS2B-NS3(S135A) were transfected in HEK293T/17 cells. At day 2 post transfection cells were harvested and proteins extracted and separated by SDS-PAGE. Proteins were transferred to membranes which were probed successively with a mouse monoclonal anti-histidine-tag antibody and an anti-GAPDH antibody. (C, D) The expression of all NS2B-NS3 constructs was normalized to GAPDH. Error bars indicate the standard error of the mean from three independent biological replicates. P value * < 0.5. Bands shown are cropped from full length gels, and full length uncropped western blots are given in the supplemental materials

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BMC Research Notes

ISSN: 1756-0500

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