Fig. 1
From: Functional integration of natural killer cells in a microfluidically perfused liver on-a-chip model
FACS analysis for CD56+CD3− NK cells in the NK cell suspension revealed high percentage of isolated NK cells derived from human blood and human liver specimen (A). Representative immunofluorescence images of NK cells (B) stained with cytotoxicity and maturation marker NKp46 in monoculture of isolated blood NK cells (green = NKp46, blue = DAPI). IFN-gamma secretion assessed by Cytometric Bead Array (CBA) assay (BD Biosciences) of isolated NK cells after 16 h of TLR-receptor agonist stimulation (LPS and Pam3CSK4) was significantly increased compared to controls (C). Figures A–C present data of at least three independent experiments, p-values are assessed by Mann Whitney test