Fig. 1

Increased binding of different clones of anti-dsDNA abs to ctDNA modified with recombinant topo I. Modification of ctDNA was performed using 75 ng topo I per µg DNA. 384-well plates were than coated with 1.5 µg of modified DNA and incubated with 30 ng of anti-dsDNA abs. The extent of anti-dsDNA binding was measured by ELISA. Bars express the mean ± SD of at least two independent experiment performed in duplicates. All probes modified with topo I showed a significant higher anti-dsDNA abs binding using the Mann-Whitney test for statistical analysis