Fig. 3

Increased binding of of anti-dsDNA abs to short oligonucleotides modified with recombinant topo I. (A) Sequence of oligonucleotides used for the expreiments. (B)-(E) Modification of oligonucleotides was performed using 100 ng topo I per µg DNA. 384-well plates were than coated with 10 or 20 µg of modified DNA and incubated with 30 ng of anti-dsDNA abs. The extent of anti-dsDNA binding was measured by ELISA. Bars express the mean ± SD of at least two independent experiment performed in duplicates. Unpaired t test with Welch’s correction was used for statistical analysis. *P<0.05, **P<0.01, ***P<0.001