Fig. 3

Screening transcriptomic analysis of ESCs, iPSCs and MEFs reveals additional candidate mediators. Analysis of a publicly available dataset of a genome-wide RNA-seq experiment containing paired transcriptomic data of ESCs, iPSCs, and MEFs (SRP046744) [45]. A Venn diagram depicting expressed genes above the threshold, showing the number of transcripts, either exclusive or shared, among the three cell types. Groups are arbitrarily numbered from I to VII depending on their size. B Exploratory cross-matching analysis between the transcript groups with a to-the-date updated and curated list of AKT1 targets from PhosphoSitePlus database. C Associated function of the genes from cross-matching expressed in PSCs (ESCs + iPSCs). D Foxa2 and Wt1 mRNA expression levels (log₂ fold change) from an RNA-seq analysis of ESCs differentiating to epiblast-like cells. Data analysis was performed in the Stem Cell Atlas data-mining platform from publicly available multi-omic data [59]