Table 1 Details of Primer sequences, PCR product size, Restriction enzyme and digested fragment size of studied gene polymorphisms
Gene | Primers sequences | PCR product size | Restriction Enzyme | Fragment size and Genotype |
|---|---|---|---|---|
HPA-1 (GpIIIa) | F:5Ź¹CTGCAGGAGGTAGAGAGTCGCCATAG-3ā | 338Ā bp | Scarf | 214/66/75 137/77/75/46 AA (Leu, Leu), AB (Leu, Pro) and BB (Pro, Pro) |
R: 5Ź¹ CTCCTCAGACCTCCACCTTGTGCTCT-3ā | Ā | Ā | ||
HPA-3 (GPIIb) | F: 5Ź¹CTC AAG GTA AGA GCT GGG TGG AAG AAA GAC-3ā | 253Ā bp | FokI | 253 AA 126/137 BB |
R: 5Ź¹CTC ACT ACG AGA ACG GGA TCC TGA AGC CTC-3ā). | Ā | Ā | ||
Factor XIII | F: 5Ź¹CATGCCTTTTCTGTTGTCTTC-3ā | 192Ā bp | DdeI | 192 Valine/Valine 161/3 Leu/Leu |
R: 5Ź¹-3ā TACCTTGCAGGTTGACGCCCCGGGGCACTA |