Table 1 Overview of Burkholderia-derived genes and blaTEM detected in BM/BP thermal and chemical amplicons
Presence of sequences with ≥ 95% identity in NCBI | Thermal amplicons | Chemical amplicons | ARDM/PCR concordance | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
BM | BP | BM | BP | BM | BP | Thermal | Chemical | |||||
n = 22 | n = 37 | ARDM | PCR | ARDM | PCR | ARDM | PCR | ARDM | PCR | |||
aac(6) | 100% | 100% | 50% (10/20) | 0% (0/8) | 14% (5/37) | 74% (14/19) | 100% (12/12) | 59% (20/34) | 100% (35/35) | 13% | 66% | |
blaABPS | 100% | 100% | 35% (7/20) | 0% (0/8) | 0% (0/37) | 47% (9/19) | 100% (12/12) | 47% (16/34) | 57% (20/35) | 33% | 52% | |
blaBP/MBL-1 | 0 | 100% | 0% (0/20) | 0% (0/8) | 5% (2/37) | 0% (0/1) | 0% (0/19) | 0% (0/12) | 71% (24/34) | 97% (34/35) | 92% | 80% |
blaBP/MBL-3 | 100% | 100% | 100% (20/20) | 100% (11/11) | 68% (25/37) | 100% (1/1) | 95% (18/19) | 100% (12/12) | 100% (34/34) | 100% (35/35) | 100% | 100% |
blaOXA-42/43 | 0% | 100% | 0% (0/20) | 0% (0/8) | 62% (23) | 100% (1/1) | 0% (0/19) | 0% (0/12) | 94% (32/34) | 100% (35/35) | 100% | 95% |
blaTMBa | 0% | 0% | 0% (0/20) | 0% (0/37) | 0% (0/19) | 0% (0/34) | ||||||
blaVEBa | 0% | 0% | 0% (0/20) | 0% (0/37) | 0% (0/19) | 0% (0/34) | ||||||
macB | 0% | 0% | 0% (0/20) | 11% (4/37) | 0% (0/19) | 85% (29/34) | ||||||
penA-BP | 100% | 100% | 65% (13/20) | 73% (8/11) | 3% (1/37) | 0% (0/1) | 89% (17/19) | 75% (9/12) | 76% (26/34) | 57% (20/35) | 67% | 68% |
penA-BCb | 0% | 0% | 0% (0/20) | 0% (0/37) | 0% (0/19) | 12% (4/34) | ||||||
qacE | 100% | 100% | 100% (20/20) | 84% (31/37) | 95% (18/19) | 100% (34/34) | ||||||
blaTEM | 0% | 100% | 35% (7/20) | 45% (10/22) | 35% (13/37) | 14% (5/37) | 0% (0/19) | 0% (0/7) | 0% (0/34) | 0% (0/5) | 77% | 100% |