Table 1 qPCR primer sequence information
mRNA | Accession no. | Amplicon (bp) | Forward primer (5´ to 3´) | Reverse primer (5´ to 3´) |
|---|---|---|---|---|
Rpl32 | NM_172086 | 66 | AACCGAAAAGCCATTGTAGAAA | CCTGGCGTTGGGATTGG |
Ttp/Zfp36/Tis11 | NM_011756 | 70 | GGTACCCCAGGCTGGCTTT | ACCTGTAACCCCAGAACTTGGA |
Zfp36l1/Tis11b | NM_007564 | 60 | TGCGAACGCCCACGAT | CTTCGCTCAAGTCAAAAATGG |
Zfp36l2/Tis11d | NM_001001806 | 77 | GAGGGCACCTCCCAACCT | TGACAGAAGTGTGGTCGACATTT |
Zfp36l3 | NM_001009549 | 70 | CGAACTGCGTACCCTGTCAAG | GCCAACGCTGTGGAAGGT |
Gm-csf | NM_009969 | 71 | CACCCGCTCACCCATCAC | GGAGGTTCAGGGCTTCTTTGA |
Cox2/Ptgs2 | NM_011198 | 106 | CCACCTCTGCGATGCTCTTC | CATTCCCCACGGTTTTGACATG |
Ifnγ | NM_008337 | 81 | TGGCATAGATGTGGAAGAAAAGAG | TGCAGGATTTTCATGTCACCAT |
Il12b | NM_008352 | 79 | GACCAGAGACATGGAGTCATAGG | TGTACTGGCCAGCATCTAGAAACT |
Tnf | NM_013693 | 74 | GCTGTCGCTACATCACTGAACCT | TGACCCGTAGGGCAATTACA |