Lipopolysaccharide regulation of antiinflammatory tristetraprolin family and proinflammatory gene expression in mouse macrophages

Table 3 LPS effect on protein content in mouse macrophages

Treatment	Time (h)	Supernatant protein			Pellet protein			Total protein
Treatment	Time (h)	Concentration (µg/µL)	Amount (mg)	Ratio (%)	Concentration (µg/µL)	Amount (mg)	Ratio (%)	Amount (mg)	Ratio (%)
DMSO	24	9.28 ± 1.04	4.64	100.00	16.85 ± 0.21	0.84	100	5.48	100
LPS	2	8.46 ± 0.23	4.23	91.16	25.43 ± 0.18 **	1.27	151	5.50	100
LPS	4	10.52 ± 0.71	5.26	124.35	20.86 ± 0.17 **	1.04	82	6.30	115
LPS	8	10.05 ± 0.90	5.77	109.70	21.59 ± 0.18 *	1.08	104	6.10	97
LPS	24	9.32 ± 0.24	4.66	80.76	19.07 ± 0.39 *	0.95	88	5.61	92

Mouse macrophages were treated with LPS (100 ng/mL) for 2, 4, 8 and 24 h. Protein content was determined with the Bradford method. The data represent the mean ± standard deviation (n = 3). “*” and “**” displayed in the Table represent significant difference between the control and the treatment at p < 0.05 and p < 0.01, respectively

ISSN: 1756-0500