- Short Report
- Open Access
Evidence for bacteriophage T7 tail extension during DNA injection
BMC Research Notes volume 1, Article number: 36 (2008)
Electron micrographs of bacteriophage T7 reveal a tail shorter than needed to reach host cytoplasm during infection-initiating injection of a T7 DNA molecule through the tail and cell envelope. However, recent data indicate that internal T7 proteins are injected before the DNA molecule is injected. Thus, bacteriophage/host adsorption potentially causes internal proteins to become external and lengthen the tail for DNA injection. But, the proposed adsorption-induced tail lengthening has never been visualized.
In the present study, electron microscopy of particles in T7 lysates reveals a needle-like capsid extension that attaches partially emptied bacteriophage T7 capsids to non-capsid vesicles and sometimes enters an attached vesicle. This extension is 40–55 nm long, 1.7–2.4× longer than the T7 tail and likely to be the proposed lengthened tail. The extension is 8–11 nm in diameter, thinner than most of the tail, with an axial hole 3–4 nm in diameter. Though the bound vesicles are not identified by microscopy, these vesicles resemble the major vesicles in T7 lysates, found to be E. coli outer membrane vesicles by non-denaturing agarose gel electrophoresis, followed by mass spectrometry.
The observed lengthened tail is long enough to reach host cytoplasm during DNA injection. Its channel is wide enough to be a conduit for DNA injection and narrow enough to clamp DNA during a previously observed stalling/re-starting of injection. However, its outer diameter is too large to explain formation by passing of an intact assembly through any known capsid hole unless the hole is widened.
A double-stranded DNA bacteriophage starts an infection by injecting its DNA genome into a host cell; progeny later assemble by packaging a genome. The protein movements that cause DNA injection are usually assumed to be restricted primarily to an external tail; the protein movements of DNA packaging are usually assumed to be restricted primarily to a multimeric DNA packaging ATPase [reviewed in references [1–3]]. However, as previously discussed , the protruding portion of the tail of bacteriophage T7 (23 nm long ) appears not long enough to reach from the outer shell of the bacteriophage, through the outer host membrane, the periplasmic space/cell wall and then the inner membrane, total distance about 24 nm [6, 7]. Thus, DNA injection appears to require lengthening of the T7 tail.
Tail lengthening is supported by the following observations: (a) After bacteriophage/host binding, five bacteriophage proteins are detected within the host cell before the DNA molecule is injected: gp6.7, gp7.3, gp14, gp15 and gp16 [8, 9]; T7 proteins are named by gp, followed by the gene number, as previously reviewed . Of these "injected" proteins, all but gp7.3  are part of an internal, tapered protein cylinder  that has an axial hole, is co-axial with the T7 tail and is surrounded by the packaged genome before injection (Figure 1). Injection of internal proteins is not unique to T7-class bacteriophages; injection of internal bacteriophage T4 proteins has also been demonstrated . (b) After the first 850–1000 DNA base pairs (with a length of 289–340 nm), T7 DNA injection stops and does not re-start until transcription initiated by an injected promoter re-starts injection. The DNA initially injected is protected from host nucleases [13, 14]. The mechanism of this protection is not known [4, 13, 14].
The injected internal proteins potentially lengthen the T7 tail for DNA injection and also possibly DNA protection after injection [8, 15]. However, evidence for such tail lengthening was not reported in the studies of references 4, 8, 9, 13 and 14. Intriguingly, a capsid has been observed with needle-like extension long enough to be a lengthened T7 tail . However, the extension was not bound to outer membrane, as it would be if injecting DNA. In the present study, a similar extension is observed by electron microscopy to attach and sometimes penetrate vesicles likely to be from the outer host membrane.
The sample for electron microscopy was obtained with a procedure of reference 11, briefly: Particles in a 6-liter lysate of T7-infected E. coli were concentrated by polyethylene glycol precipitation. The precipitate was resuspended and centrifuged through a cesium chloride step gradient (profile of light scattering: Figure 2a). The fraction of the step gradient indicated by a bracket in Figure 2a was subjected to buoyant density centrifugation in a cesium chloride density gradient (profile of light scattering: Figure 2b). The density gradient of Figure 2b had the following features: The lower of two bands near the top of the gradient was produced by the T7 procapsid (also called capsid I; labeled CI in Figure 2b; density = 1.283 g/ml). Capsid I appears before DNA packaging occurs and is converted to a more bacteriophage-like capsid (capsid II) during DNA packaging [1, 3]. The second band near the top of the gradient was produced by capsid II (labeled CII in Figure 2b; density = 1.272 g/ml) (capsid identification not shown). Lower bands were produced by dimeric capsid/bacteriophage particle aggregates (dimer; density = 1.398 and 1.411 g/ml) in Figure 2b; the lowest band was produced by bacteriophage particles (ϕ in Figure 2b).
Electron microscopy revealed that the region between CII and dimers in Figure 2b had vesicle-like particles, possibly host outer membrane vesicles [16, 17]. Thus, gradient fractions were screened for bacteriophage capsids bound to vesicle-like particles in a complex that had the following characteristics expected of a DNA injection complex (IC): (a) either a bacteriophage tail or a lengthened tail connected bacteriophage to vesicle-like particle (to be called a vesicle) and (b) DNA was at least partially emptied from the capsid. Apparent IC's were found concentrated in the region of the gradient indicated with the bracket labeled IC and coincident with a band of light scattering in Figure 2b (1.359 g/ml).
These apparent IC's each consisted of (a) a vesicle of variable size and shape and (b) one or more icosahedral T7 capsids. Figure 3 has an electron microscope field with several apparent IC's. Arrow 1 in Figure 3 indicates a non-spherical vesicle that, at the top, has bound two T7 capsids (indicated by a bracket), both apparently vesicle-attached via a needle-like capsid extension (white arrowheads) that is slightly (10–15%) longer than the 23 nm T7 tail. Both these capsids also appear to have internal material. The amount of the internal material is less than in mature bacteriophage particles, based on comparison to the bacteriophage particles indicated by arrows 2 and 3. However, the bracket-marked capsids were not empty, based on comparison to the apparently empty capsid indicated by arrow 4. Thus, the conclusion is drawn that the bracket-marked capsids have DNA, but in amount less than the amount in a bacteriophage particle, as expected for an IC.
The bacteriophage particles indicated by arrows 2 and 3 in Figure 3 have tails (note the tapering) and are attached via their DNA-containing outer shells (not the tails) to an object previously characterized [5, 18] as a product (called polycapsid) of mis-assembly of the major protein (gp10) of the capsid's outer shell. The polycapsids have vesicles within them, a point previously noted [5, 18]. The polycapsid-adsorbed bacteriophages have not injected DNA and this adsorption is assumed not to have significance for biological injection.
For the two bracket-marked capsids in Figure 3, the needle-like extension appears less tapered than the tail, an observation also made for all 27 other vesicle attached extensions observed. The total extension length cannot be determined for the bracket-marked capsids because of partial obscuring of part of the extension, especially by material inside of the vesicle. Although partially obscured by this material, the extension of the left bracket-marked capsid in Figure 3 does appear to extend in a straight line through the proximal surface of the vesicle, completely to the distal vesicle surface, producing a total length of 40–50 nm.
The needle-like extension associated with the capsid indicated by arrow 5 of Figure 3 (magnified in the inset) is seen more clearly to penetrate the attached vesicle and has a length of 41 nm, but also appears to have an additional ~14 nm broken from the extension at the distal inner surface of the vesicle (black arrowhead in Figure 3 and inset). The high visibility within the vesicle is apparently caused by vesicle breakage/leakage that is indicated by the discontinuity of the vesicle's boundary. The capsid of this complex is partially full of DNA. The outer diameter of the needle is 8–11 nm, significantly smaller than the diameter of the wider end of the tail. A central channel, 3–4 nm in diameter, is sometimes visible, as illustrated by the more magnified complexes in Figure 4a–d. Thirty-five needle-like extensions, all with diameter in this range, were observed. Some were on capsids that were not attached to vesicles. Some had lengths of 50–60 nm (Figure 4e). Most had extensions that were shorter, possibly broken (Figure 4f).
When analyzed by non-denaturing agarose gel electrophoresis, the vesicle-containing fractions of the gradient in Figure 2b all produced a single, broad "vesicle" band that was further from the origin than capsids (not shown). To characterize the vesicles, vesicle proteins were analyzed after vesicle purification by both the centrifugation of Figure 2a, b and then preparative non-denaturing two-dimensional agarose gel electrophoresis (2d-AGE). The sample for 2d-AGE was a solution of Alexa 488-stained particles with a density of 1.265 g/ml (density of capsid II). The procedure of 2d-AGE uses a dilute (0.3%) gel for the first dimension and a more concentrated (1.8%) gel for the second; details are described and reviewed in reference 19.
The 2d-AGE fractionates primarily by average electrical surface charge density (σ) in the first dimension and by both σ and effective radius (RE) in the second dimension. The value of RE is a decreasing function of the angle (θ) subtended by the direction of the first field and a line drawn from the origin to the position of a particle in the gel. The line for a RE of 26.1 nm is drawn in Figure 5a. For this analysis, the vesicles were produced by use of a T7 mutant that assembles capsid I, but does not package DNA and does not convert capsid I to capsid II. Thus, complications from the presence of bacteriophage particles were minimized.
The fluorescence profile of Alexa 488-stained particles after 2d-AGE had primarily two features. (a) A band of a capsid not further investigated had a small percentage of the fluorescence (arrow inside Figure 5a). (b) A streak of vesicles had most fluorescence (bracket with capital letters in Figure 5a). The RE standard is capsid I (boxed band in Figure 5a). The capsid I had been run in parallel in a gel embedded in the same agarose frame (different quadrant) in which the gel of Figure 5a was embedded ; the image of the capsid I band was then overlaid on the image of Figure 5a. A streak of the type observed above for vesicles is expected for particles that are uniform in σ and variable in RE . The range of RE values for streak-producing particles was determined by quantification of θ values at the two ends of the streak and was found to be 20–50 nm, within the range of values previously found for E. coli outer membrane vesicles (10–100 nm ).
For further analysis, the gel at the position of the streak of Figure 5a was cut into five slices, indicated by letters in Figure 5a. The slices were boiled and the proteins were analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDSPAGE). After staining with SYPRO Ruby protein gel stain (Figure 5b), the proteins of the indicated bands were excised, digested in situ with trypsin and identified by HPLC-electrospray-tandem mass spectrometry. The two major proteins identified for slices A-E were ompA  and ompF , as indicated in Figure 5b; these proteins were not detected in several non-staining areas of the agarose gel of Figure 5a that were analyzed (not shown).
The next most abundant protein was the 60 Kd chaperonin (indicated by chaperonin in Figure 5b). However, this protein was found in several locations in the agarose gel, even where no band or streak was detected. The 60 Kd chaperonin is, therefore, not significantly associated with the vesicles. Additional, vesicle-associated proteins were ferrichrome iron receptor protein, product of the FhuA gene (FhuA in Figure 5b)  and ferrienterobactin outer-membrane receptor precursor, product of the FepA gene (FepA in Figure 5b) . OmpA, ompF, FhuA and FepA proteins are all E. coli outer membrane proteins. Thus, we conclude that the vesicles are formed from the E. coli outer membrane.
The length of the observed needle-like extension is great enough to more than traverse the host's outer membrane, periplasmic space/cell wall and inner membrane. Thus, we hypothesize that this extension lengthens the tail for DNA injection and that the extension contains at least some of the proteins that others [8, 9] have found to be injected. The small size of its channel supports the hypothesis  that a constriction causes the pre-transcription delay of injection. The extension is too short to directly explain protection of the initially injected 850–1,000 base pairs.
If gp6.7, gp14, gp15 and gp16 are part of the lengthened tail, the question remains: Did these proteins cross the capsid's outer shell while assembled as in Figure 1? If so, were the assembled proteins unfolded, as previously proposed ? Or, did these proteins cross as single protein molecules and subsequently assemble to form the needle? If the proteins cross while both assembled and folded, the outer shell/tail connector (a ring of gp8; Figure 1) would have to widen its central hole that varies from 3.5–4.5 nm in diameter . In either case, formation of the tail extension would require protein motion not previously observed.
Fujisawa H, Morita M: Phage DNA packaging. Genes Cells. 1997, 2: 537-545. 10.1046/j.1365-2443.1997.1450343.x.
Rossmann MG, Mesyanzhinov VV, Arisaka F, Leiman PG: : The bacteriophage T4 DNA injection machine. Curr Opin Struct Biol. 2004, 14: 171-80. 10.1016/j.sbi.2004.02.001.
Johnson JE, Chiu W: DNA packaging and delivery machines intailed bacteriophages. Curr Opin Struct Biol. 2007, 17: 237-243. 10.1016/j.sbi.2007.03.011.
Grayson P, Molineux IJ: Is phage DNA 'injected' into cells- biologists and physicists can agree. Curr Opin Microbiol. 2007, 10: 401-409. 10.1016/j.mib.2007.04.004.
Steven AC, Trus BL: The structure of bacteriophage T7. Electron Microscopy of Proteins, Viral Structure. Edited by: Harris JR, Horne RW. 1986, London: Academic Press, 5: 1-33.
Bai W, Zhao K, Asami K: Effects of copper on dielectricproperties of E. coli cells. Colloids Surfaces B: Biointerfaces. 2007, 58: 105-115. 10.1016/j.colsurfb.2007.02.015.
Costerton JW, Ingram JM, Cheng KJ: Structure and function of the cell envelope of ram-negative bacteria. Bacteriol Rev. 1974, 38: 87-110.
Molineux IJ: No syringes please, ejection of phage T7 DNA from the virion is enzyme driven. Mol Microbiol. 2001, 40: 1-8. 10.1046/j.1365-2958.2001.02357.x.
Kemp P, Garcia LR, Molineux IJ: Changes in bacteriophage T7 virion structure at the initiation of infection. Virology. 2005, 340: 307-317. 10.1016/j.virol.2005.06.039.
Studier FW, Dunn JJ: Organization and expression of bacteriophage T7 DNA. Cold Spring Harbor Symp Quant Biol. 1983, 47: 999-1007.
Serwer P: Internal proteins of bacteriophage T7. J Mol Biol. 1976, 107: 271-291. 10.1016/S0022-2836(76)80005-8.
Bair CL, Rifat D, Black LW: Exclusion of glucosyl-hydroxymethylcytosine DNA containing bacteriophages is overcome by the injected protein inhibitor IPI*. J Mol Biol. 2007, 366: 779-789. 10.1016/j.jmb.2006.11.049.
Moffatt BA, Studier FW: Entry of bacteriophage T7 DNAinto the cell and escape from host restriction. J Bacteriol. 1988, 170: 2095-2105.
Struthers-Schlinke JS, Robins WP, Kemp P, Molineux IJ: The internal head protein Gp16 controls DNA ejection from the bacteriophage T7 virion. J Mol Biol. 2000, 301: 35-45. 10.1006/jmbi.2000.3940.
Serwer P: Observation of DNA by negative staining: phage T7 DNA-capsid complexes. Ninth International Congress on Electron Microscopy II. 1978, 228-229.
Lavigne R, Noben JP, Hertveldt K, Ceyssens PJ, Briers Y, Dumont D, Roucourt B, Krylov VN, Mesyanzhinov VV, Robben J, Volckaert G: The structural proteome of Pseudomonas aeruginosa bacteriophage ϕKMV. Microbiology. 2006, 152: 529-534. 10.1099/mic.0.28431-0.
Lee EY, Bang JY, Park GW, Choi DS, Kang JS, Kim HJ, Park KS, Lee JO, Kim YK, Kwon KH, Kim KP, Gho YS: Global proteomic profiling of native outer membrane vesicles derived from Escherichia coli. Proteomics. 2007, 7: 3143-3153. 10.1002/pmic.200700196.
Serwer P: Fibrous projections from the core of a bacteriophage T7 procapsid. J Supramol Struct. 1979, 11: 321-326. 10.1002/jss.400110307.
Serwer P, Khan SA, Griess GA: Non-denaturing gel electrophoresis of biological nanoparticles: viruses. J Chromatogr A. 1995, 698: 251-261. 10.1016/0021-9673(94)01259-H.
Smith SG, Mahon V, Lambert MA, Fagan RP: A molecular Swiss army knife: OmpA structure, function and expression. FEMS Microbiol Lett. 2007, 273: 1-11. 10.1111/j.1574-6968.2007.00778.x.
Nguyen TX, Alegre ER, Kelley ST: Phylogenetic analysis ofgeneral bacterial porins: a phylogenomic case study. J Mol Microbiol Biotechnol. 2006, 11: 291-301. 10.1159/000095631.
Bonhivers M, Desmadril M, Moeck GS, Boulanger P, Colomer-Pallas A, Letellier L: Stability studies of FhuA, a two-domain outer membrane protein from Escherichia coli. Biochemistry. 2001, 40: 2606-2613. 10.1021/bi001725i.
Ma L, Kaserer W, Annamalai R, Scott DC, Jin B, Jiang X, Xiao Q, Maymani H, Massis LM, Ferreira LC, Newton SM, Klebba PE: Evidence of ball-and-chain transport of ferric enterobactin through FepA. J Biol Chem. 2007, 282: 397-406. 10.1074/jbc.M605333200.
Agirrezabala X, Martin-Benito J, Caston JR, Miranda R, Valpuesta JM, Carrascosa JL: Maturation of phage T7 involves structural modification of both shell and inner core components. EMBO J. 2005, 24: 3820-3829. 10.1038/sj.emboj.7600840.
Thomas JA, Hardies SC, Rolando M, Hayes SJ, Lieman K, Carroll CA, Weintraub ST, Serwer P: Complete genomic sequence and mass spectrometric analysis of highly diverse, atypical Bacillus thuringiensis phage 0305ϕ8–36. Virology. 2007, 368: 405-421. 10.1016/j.virol.2007.06.043.
For support, we thank the National Institutes of Health (GM24365 to PS), the Welch Foundation (AQ-764 to PS) and The Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation (PS). Protein identifications were conducted in the UTHSCSA Institutional Mass Spectrometry Laboratory.
The authors declare that they have no competing interests.
PS conceived the study, designed the preparative protocols, performed the electron microscopy and wrote the manuscript. EW performed the preparative work. KH performed the mass spectrometry. STW directed the mass spectrometry laboratory and assisted in writing the manuscript. All authors read and approved the final manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Serwer, P., Wright, E.T., Hakala, K.W. et al. Evidence for bacteriophage T7 tail extension during DNA injection. BMC Res Notes 1, 36 (2008). https://doi.org/10.1186/1756-0500-1-36
- Bacteriophage Particle
- Cesium Chloride
- Outer Membrane Vesicle
- Injection Complex
- Cesium Chloride Density Gradient