Figure 2
From: Heterologous production of human papillomavirus type-16 L1 protein by a lactic acid bacterium
Expression of HPV-16 L1 protein by recombinant L. lactis. A) L. lactis strains were grown and induced with 1 ng/ml of nisin for 1 h. After centrifugation, non-induced (- nis) and induced (+ nis) culture samples were treated as described in materials and methods and L1 production and secretion analyzed by Western blot. L. lactis strain contains pCYT:L1 plasmid encoding for a cytoplasmic form of L1 and L. lactis strain contains pSEC:L1 encoding for the precursor preL1 (i.e. SPUsp45 fused to L1). B) L. lactis (pSEC:L1) strain was grown and induced with 10 ng/ml of nisin for 1 h. After centrifugation, induced (+ nis) culture samples were treated as described in results and discussion and L1 production and secretion analyzed by Western blot. Arrows indicate positions of L1 mature and precursor forms. Abbreviations: C, cell lysates; S, supernatant fraction; M, positions and sizes of molecular mass markers.