Heterologous production of human papillomavirus type-16 L1 protein by a lactic acid bacterium

BMC Research Notes

Table 1 Bacterial strains and plasmids used.

Strains	Genotype		Reference
E. coli TG1	supE, hsd, Δ5, thi, Δ(lac-proAB), F'(traD36 proAB-lacZΔM15)		[30]
L. lactis MG1363	Wild type, plasmid free		[29]
L. lactis NZ9000	MG1363 (nisRK genes into chromosome), plasmid free		[27]
Plasmids	Replicon	Plasmid characteristics; cloned cassettes characteristics	Reference
pcDNA3-L1	ColE1	Ap^r, plasmid containing the HPV-16 L1 sequence	[31]
pCR:TOPO	ori pUC	Ap^r	Invitrogen
pCR:TOPO:L1	ori pUC	Ap^r, plasmid containing HPV-16 L1 sequence without ATG start codon	This work
pCYT:E7	pWV01	Cm^r; gene, expressed from P_nisA encodes E7	[10]
pSEC:E7	pWV01	Cm^r; gene, expressed from P_nisA encodes SP_Usp-E 7 precursor	[10]
pCYT:L1	pWV01	Cm^r; gene, expressed from P_nisA encodes native L1 protein	This work
pSEC:L1	pWV01	Cm^r; the gene expressed from P_nisA encodes SP_Usp-L 1 precursor	This work

ISSN: 1756-0500