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Table 1 Bacterial strains and plasmids used.

From: Heterologous production of human papillomavirus type-16 L1 protein by a lactic acid bacterium

Strains Genotype Reference
E. coli TG1 supE, hsd, Δ5, thi, Δ(lac-proAB), F'(traD36 proAB-lacZΔM15) [30]
L. lactis MG1363 Wild type, plasmid free [29]
L. lactis NZ9000 MG1363 (nisRK genes into chromosome), plasmid free [27]
Plasmids Replicon Plasmid characteristics; cloned cassettes characteristics Reference
pcDNA3-L1 ColE1 Apr, plasmid containing the HPV-16 L1 sequence [31]
pCR:TOPO ori pUC Apr Invitrogen
pCR:TOPO:L1 ori pUC Apr, plasmid containing HPV-16 L1 sequence without ATG start codon This work
pCYT:E7 pWV01 Cmr; gene, expressed from PnisA encodes E7 [10]
pSEC:E7 pWV01 Cmr; gene, expressed from PnisA encodes SPUsp-E 7 precursor [10]
pCYT:L1 pWV01 Cmr; gene, expressed from PnisA encodes native L1 protein This work
pSEC:L1 pWV01 Cmr; the gene expressed from PnisA encodes SPUsp-L 1 precursor This work