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Investigating effects of parasite infection on body condition of the Kafue lechwe (Kobus leche kafuensis) in the Kafue basin
© Munang'andu et al; licensee BioMed Central Ltd. 2010
- Received: 2 August 2010
- Accepted: 23 December 2010
- Published: 23 December 2010
The Kafue lechwe (Kobus leche Kafuensis), a medium-sized semi-aquatic antelope, is endemic to the Kafue basin of Zambia. The population of the Kafue lechwe has significantly dropped in the last decades leading to its subsequent inclusion on the red list of endangered species. In order to save the remaining population from extinction, it has become increasingly important that the impact of parasite infection and infestation on the Kafue lechwe is investigated.
Endoparasites accounted for the majority of parasites observed from a study of 40 Kafue lechwe occurring in the the Kafue basin. Amphistoma spp. were present in all animals examined, while Fasciola gigantica had a prevalence rate of 0.525 (95% CI: 0.36 to 0.69) and species of Schistosoma 0.3 (95% CI: 0.15 to 0.45). Among the ectoparasites, Strobiloestrous vanzyli, had a prevalence rate of 0.15 (95% CI: 0.03 to 0.27), while Rhipicephalus appendiculatus had a prevalence of 0.075 (3/40). Our findings indicate that body condition was not influenced by the parasitic infestation in Kafue lechwe. There was no association between sex and parasitic burden (odds ratio = 0.3, 95% CI: 0.8-1.3). However, an association between age and parasitic burden was observed as older animals above 15 years were more likely to get parasite infections than those aged between 1-5 years (odds ratio = 1.5, 95% CI: 1.1-2.4).
Overall, there was no evidence that parasitic infections and infestations adversely affected the lechwe population on the Kafue basin. These findings indicate that ecto- and endo-parasite infestation might not play a significant role in reducing the Kafue lechwe population on the Kafue basin.
- Body Condition
- Parasitic Burden
- Tick Infestation
The Kafue lechwe antelope (Kobus leche kafuensis) is the predominant wildlife species of the Kafue basin. These antelopes are lek-breeders and share their habitat with other wild and domestic species. They are semi-aquatic and medium sized antelopes that live in large groups close to water bodies or marsh places . The lechwe population on the Kafue basin has steadily declined from an estimated 80,000 in 1975 to 41,000 in 2001 [2, 3] leading to its recent inclusion on the IUCN Red list of threatened species . This has led the Zambia wildlife Authority (ZAWA) to embark on conservation programs aimed at saving the remaining population from going into extinct. In order to achieve this, it has become increasingly important to investigate all potential factors likely to contribute to the decline of the Kafue lechwe population.
The Kafue basin ecosystem harbors various species of ecto- and endo parasites that infest both wild and domestic animals [5–7]. Studies by Kampamba  and Kapungwe  indicate that ecological changes and animal diseases are the major factors reducing the Kafue lechwe population in the Kafue basin. Gallagher et al  estimated that parasitic diseases accounted for approximately 14% annual mortality of the lechwe population each year and formed a major factor limiting population growth. Previous studies have focused on identifying the species of parasites infesting the Kafue lechwe [9, 10], did not investigate on the impact of these parasites. In cattle parasitic infestations have been associated with considerable weight loss, which in some cases have led to mortalities depending on the severity of the infection . In evaluating animal health conditions, body-condition is used as a proxy for evaluating the health status of wildlife within a habitat and is thus a useful tool for assessing the impact of diseases on wild species . In the present study, we investigated the effect of the ecto and endo-parasites found in the Kafue basin on body condition of free ranging Kafue lechwe, as a way of examining the role of parasite infestation on reducing the Kafue lechwe population.
Study area and animals
Given the endangered status of the Kafue lechwe , only 40 animals (20 females and 20 males) were sacrificed by ZAWA for the purpose of parasite investigations between October and December 2005. Ethical approval, under special license number 00113, was issued by the Zambia Wildlife Authority the sole custodians of wildlife in Zambia as stipulated in Section 37 of the Zambia Wildlife Act of the laws of the Republic of Zambia. Animals were aged using ring patterns on the horns as well as tooth development and wear [15, 16]. Examination of carcasses was carried out as described by Gracey . Body condition was graded using the kidney fat index (KFI) as described by Riney [18, 19]. The amount of fat removed from the kidneys was weighed and divided by the total weight of the kidneys for each animal to get the index. Each carcass was allocated a score of 1 (poor: almost no fat); 2 (average: fair fat amount present); or 3 (good: plentiful fat, completely obscuring the kidneys).
Sample collection and examination
All animals were eviscerated followed by visual inspection and digital palpation of all body organs. Contents of the thoracic cavity were examined separately. Each component of the digestive system was processed separately with the contents from each segment being placed in separate bottles for laboratory examination. Schistosoma matteii in the mesenteries were collected using forceps and counted at the sampling sites. The liver was incised and examined for the presence of flukes. Visceral contents from each organ were emptied into a bucket and the mucosa washed thoroughly in water with firm digital pressure.
Quantification of the parasites was achieved by sieving and sedimentation following the method described by Taira et al, while egg counts of Schistosoma matteii were examined as described by Lawrence . Cysts were excised from the heart muscles and evaginated from cyst membranes using methods described by Edgar  and Gonzalez et al . They were later identified as cysticercus of Taenia saginata as described by Okafor  and Opara et al .
All observations were recorded in Microsoft Excel and later transferred to STATA™ V/SE 10 (Stata Corp, http://www.stata.com) for analysis. Logistic regression was used to determine the associations between body condition (based on the KFI) and parasite infection while the ROC test was used to determine the reliability of the model. Pearson's chi-square was used to determine the correlation of concurrent infections between different parasites.
Crude parasitic infection in lechwe antelopes across the two sampling areas (Lochinvar and Blue Lagoon) (n = 40), indicative of crude prevalence values per type of parasite (October to December, 2005)
No. of Lechwes tested
Proportion of Positive findings(%)
95% Confidence Interval
Factors assessed in relation to parasite infestation in the Kafue lechwe antelopes (n = 40), showing proportions of distribution according to various levels (October to December, 2005).
Body Condition Score
Fasciola gigantica was the second most prevalent endo-parasite at a proportion of 52.5% (21/40) after species of Amphistosoma found in virtually all animals screened. Concurrent infections of Amphistosoma and Schistosoma mattei accounted for 17.5% (7/40), species of Amphistosoma and Fasciola gigantica 20% (8/40), Schistosoma mattei and Fasciola gigantica 17.5% (8/40), while a combination of all trematodes (trematodes Amphistosoma species, Fasciola gigantica and Schistosoma mattei) accounted for 12.5% (5/40). Co-infections of all trematodes and nematodes (Setaria spp.) accounted for 5% (2/40), while a combination of trematodes, nematodes and cestodes (cysts) accounted for 2.5% (1/40). There was a correlation (r = 0.361, p < 0.022) between amphistosome and schistosome infections.
Logistic regression analysis
Results from a predictive logistic regression model for parasitic infection in Kafue lechwe antelopes (n = 40) -October to December, 2005.
Body C score
In ecological management, monitoring body condition of wild game is an important tool for monitoring the nutritional conditions of the habitat, and such information can also be used to assess the negative effect of parasites on the host . Although several techniques for estimating body condition in wild ungulates have been developed [26, 27], the kidney fat index (KFI) is the most widely used technique because it is regarded as the most reliable indicator of body condition in wild game . Hence, in the present study, we investigated the impact of worm infestations on body condition of the Kafue lechwe by using the KFI as a proxy measure. Stien et al  pointed out that timing in carrying out observational studies aimed at detecting the impact of worm infestation on susceptible hosts is crucial because it is important to take into account the lag phase between the time of infection and the maximum parasite intensity in the infected hosts. Phiri et al  observed that increase in worm infection in the Kafue basin begins in June/July when the floods recede extending up to November/December when the rain season begins on the Kafue basin. Taking these observations into account, we carried out our investigations between October and December during the peak of the dry season when the parasite intensity is expected to be high in the infected animals. We only managed to evaluate 40 Kafue lechwe antelopes because these wild animals are protected by law because of the endangered nature of the Kobus leche kafuensis subspecies .
Our findings indicate that there was no statistically significant association between KFI and parasitic burden (Table 3), suggesting that the observed parasitic infections were unlikely to cause adverse body conditions in the infected animals. These findings suggest that at present, parasitic infections might not play a pivotal role to lechwe debilitation and consequently to population reduction on the Kafue basin. This is contrary to observation made by Gallagher et al , who reported that worm infestation was estimated to kill about 14% of the Kafue lechwe in the Kafue basin and suggested that worm infections were likely to be the major cause of the population reduction observed in the Kafue lechwe antelopes. However, age was shown to have a significant effect, as animals older than 15 years were one and half times more likely to carry parasites than those aged between 1 and 5 years (odds ratio = 1.5, 95% CI: 1.1-2.4). Sex had no influence on the parasitic burden. Gallagher et al  recorded a higher prevalence of fasciolosis in females than males, while Stafford  recorded a higher prevalence in the males than the females. The variations obtained from the two studies [9, 10] suggest that sex has no influence on parasitic burdens in lechwe and this supports our current findings.
Tick infestation rates of 17.5% (7/40) in our study are lower than those associated with low productivity and loss of weight gain in cattle [29, 30]. This could be attributed to the semi aquatic nature of the lechwe, which spends most of its time in water submerged up to shoulder. It is likely that the aquatic tendency might not support the survival of most ticks. Similarly, Stafford  recorded a low prevalence of tick infestation on lechwe in the Kafue basin. Apart from ticks, oestrid larvae (Strobiloestrous vanzyli) were also observed in lechwe. These parasites are common in lechwe, and their presence has not been linked to mortalities in lechwe .
This study highlights species of parasites present in the Kafue lechwe antelope and provides a broad assessment of body condition relative to parasite loads. Based on these comparisons, parasite load does not appear to have a significant impact on the health and condition of lechwe. Therefore, factors other than parasites should be considered in future investigations designed to evaluate factors most important to maintenance of a viable lechwe population in the Kafue basin.
The authors are grateful to the Zambia Wildlife Authority (ZAWA) for providing the study animals and the School of Veterinary Medicine at the University of Zambia (UNZA) in Lusaka for their kind support by providing laboratory equipment and materials used in the study.
- Sayer JA, van Lavieren LP: The ecology of the Kafue lechwe population of Zambia before the operation of the hydroelectricity dams on the Kafue river. E Afr Wildl J. 1975, 13: 9-37.View ArticleGoogle Scholar
- Kamweneshe B, Beilfuss R, Simukonda C: Population and distribution of Kafue lechwe and other large mammals on the Kafue flats, Zambia. 2002, Report to World Wide Fund for nature and Partners for WetlandsGoogle Scholar
- Kapungwe EM: Carrying capacity changes and management of the Kafue lechwe (Kobus leche kafuensis) in Kafue Flats, Zambia. 1993, MSc Thesis. University of Zimbabwe, Harare, ZimbabweGoogle Scholar
- IUCN red list of threatened species. Version 2009.1: IUCN SSC antelope specialist group 2008. Kobus leche. 2009, [http://www.iucnredlist.org]Google Scholar
- Phiri AM, Phiri IK, Chota A, Monrad J: Trematode infections in freshwater snails and cattle from the Kafue wetlands of Zambia during a period of highest cattle-water contact. J Helminthol. 2007, 81: 85-92. 10.1017/S0022149X07387786.PubMedView ArticleGoogle Scholar
- Speybroeck N, Madder M, Van Den Bossche P, Mtambo J, Berkvens N, Chaka G, Mulumba M, Brandt J, Tirry L, Berkvens D: Distribution and phenology of ixodid ticks in southern Zambia. Med Vet Entomol. 2002, 16: 430-41. 10.1046/j.1365-2915.2002.00397.x.PubMedView ArticleGoogle Scholar
- Wright CA, Southgate VR, Howard GW: Life-cycles of some amphistome flukes in Zambia. J Helminthol. 1979, 53: 251-2. 10.1017/S0022149X00006039.View ArticleGoogle Scholar
- Kampamba G: Ecology of kafue lechwe (Kobus Leche Kafuensis):The influences of resource limitation, disease and poaching on population regulation. 1998, Harare, Zimbabwe: University of ZimbabweGoogle Scholar
- Gallagher J, Macadam I, Sayer J, van Lavieren LP: Pulmonary tuberculosis in free living lechwe in Zambia. Trop Anim Health Prod. 1972, 4: 213-10.1007/BF02360112.View ArticleGoogle Scholar
- Stafford KJ: A review of diseases of parasites of the Kafue lechwe (Kobus-Leche-Kafuensis). J Wildl Dis. 1991, 27: 661-7.PubMedView ArticleGoogle Scholar
- Forbes AB, Huckle CA, Gibb MJ, Rook AJ, Nuthall R: Evaluation of the effects of nematode parasitism on grazing behaviour, herbage intake and growth in young grazing cattle. Vet Parasitol. 2000, 90: 111-8. 10.1016/S0304-4017(00)00218-1.PubMedView ArticleGoogle Scholar
- Wilson K, Bjornstad ON, Dobson AP, Merler S, Poglayen G, Randolph SE, Read AF, Skorping A: Heterogeneities in macroparasite infections: patterns and processes. In: The ecology of wildlife diseases. Edited by: Hudson PJ, Rizzoli A, Grenfell BT, Heesterbeek H, Dobson AP. 2002, Oxford University Press, New YorkGoogle Scholar
- Balkrishna M, Ndhlovu DE: Wildlife utilization and local people; A case study in Upper Lupande game management area, Zambia. Environ Conserv. 1992, 19: 135-44. 10.1017/S0376892900030617.View ArticleGoogle Scholar
- Lewis D, Kaweche GB, Mwenya A: Wildlife conservation outside protected areas - lessons from an experiment in Zambia. Conservation Biology. 1990, 4: 171-80. 10.1111/j.1523-1739.1990.tb00106.x.View ArticleGoogle Scholar
- Grimsdell JJR: Age determination of the African buffalo, Syncerus caffer sparrman. E Afri Wildl J. 1973, 11: 54-Google Scholar
- Jolles AE: Population biology of African buffalo (Syncerus caffer) at Hluhluwe-iMfolozi Park, South Africa. Afr J Ecol. 2007, 45: 398-406. 10.1111/j.1365-2028.2006.00726.x.View ArticleGoogle Scholar
- Gracey JF: Meat hygiene service. Vet Rec. 1991, 129: 204-10.1136/vr.129.9.204-a.PubMedView ArticleGoogle Scholar
- Riney T: Evaluating condition of free-ranging red deer (Cervus elephas), with special reference to New Zealand. N Z J Sci Tech. 1955, 36: 439-63.Google Scholar
- Riney T: A field technique for assessing physical condition in some ungulates. J Wildl Man. 1960, 24: 92-4. 10.2307/3797362.View ArticleGoogle Scholar
- Taira R, Suzuki K, Boray JC: Detection and quantification of Faciola eggs in cattle using the beads technique. Jpn J Vet Parasitol. 1983, 32: 279-86.Google Scholar
- Lawrence JA: Examination of ruminal faeces for schistosome eggs. Rhodesia Vet J. 1970, 1: 49-52.Google Scholar
- Edgar SA: Artificial evagination of larval tapeworms. Tans Kans Acad Sci. 1940, 43: 397-9. 10.2307/3625549.View ArticleGoogle Scholar
- Gonzalez AE, Falcon N, Gavidia C, Garcia HH, Tsang VC, Bernal T, Romero M, Gilman RH: Treatment of porcine cysticercosis with oxfendazole: a dose-response trial. Vet Rec. 1997, 141: 420-2. 10.1136/vr.141.16.420.PubMedView ArticleGoogle Scholar
- Okafor FC: Epizootiology of Cysticercus bovis in Imo State, Nigeria. Angew Parasitol. 1988, 29: 25-30.PubMedGoogle Scholar
- Opara MN, Ukpong UM, Okoli IC, Anosike JC: Cysticercosis of slaughter cattle in southeastern Nigeria. Impact of Emerging Zoonotic Diseases on Animal Health. 2006, 1081: 339-46.Google Scholar
- Takatsuki S: Kidney fat and marrow fat indices of the sika deer population at Mount Goyo, northern Japan. Ecol Res. 2000, 15: 453-7. 10.1046/j.1440-1703.2000.00365.x.View ArticleGoogle Scholar
- Stevenson RD, Woods WA: Condition indices for conservation: new uses for evolving tools. Integr Comp Biol. 2006, 46: 1169-90. 10.1093/icb/icl052.PubMedView ArticleGoogle Scholar
- Stien A, Irvine RJ, Ropstad E, Halvorsen O, Langvatn R, Albon SD: The impact of gastrointestinal nematodes on wild reindeer: experimental and cross-sectional studies. J Anim Ecol. 2002, 71: 937-45. 10.1046/j.1365-2656.2002.00659.x.View ArticleGoogle Scholar
- Pegram RG, Lemche J, Chizyuka HG, Sutherst RW, Floyd RB, Kerr JD, McCosker PJ: Effect of tick control on liveweight gain of cattle in central Zambia. Med Vet Entomol. 1989, 3: 313-20. 10.1111/j.1365-2915.1989.tb00234.x.PubMedView ArticleGoogle Scholar
- Pegram RG, Chizyuka HG: The impact of natural infestations of ticks in Zambia on the productivity of cattle and implications for tick control strategies in central Africa. Parasitol. 1990, 32: 165-75.Google Scholar
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