- Research article
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BK viremia in critically ill surgical patients with hemorrhagic or septic shock
BMC Research Notes volume 5, Article number: 100 (2012)
Infections with polyomavirus BK virus (BKV) are a common cause of renal dysfunction after renal transplantation and may also be harmful in surgical patients with shock. The aim of the present study was to determine the frequency of BKV viremia in critically ill surgical patients with septic or hemorrhagic shock, and, if viremia is detectable, whether viremia may be associated with renal dysfunction.
A total of 125 plasma samples from 44 critically ill surgical patients with septic or hemorrhagic shock were tested by real-time polymerase chain reaction (PCR) for BKV DNA during their stay on the intensive care unit (ICU). BKV viremia occurred in four patients, i.e. in three of the septic and in one of the hemorrhagic shock group. There was no association between viremia and renal dysfunction. All positive samples contained a low viral load (< 500 copies/ml).
Since BK viremia was rarely found and with low viral load only in critically ill surgical patients with shock, it is very unlikely that BK viremia results in BK nephropathy later on.
Infections with BK virus (BKV) are an increasing problem and a common cause of renal dysfunction after allograft renal transplantation [1, 2]. IgG seroprevalence for BK virus (BKV) was 82% in healthy blood donors, and, over a period of 14 months, no BKV viruria was detectable . Kaneko and colleagues reported prevalence of BKV in urine samples in about 13.5% of the healthy control group, 33.3% of patients with chronic renal disease, and 55.6% of patients with renal disease under cortisone therapy .
Critically ill nonimmunosuppressed patients with either trauma or sepsis have been reported to demonstrate immunologic features consistent with immunosuppression ("immunoparalysis"), i.e. cytomegalovirus or herpes simplex virus reactivation or infections [5–8]. Thus, we hypothesized that BKV viremia might also occur in critically ill surgical patients. Since the appearance of a BKV nephropathy was strongly associated with the viral load detected in renal transplant patient's blood , we focused on detection of viral load in the blood of surgical critically ill patients. The aim of the present study was to determine the frequency of BKV viremia in surgical critically ill patients with hemorrhagic or septic shock.
Patients and data collection
The prospective study is in compliance with the Helsinki declaration and was approved by the Independent Ethics Committee of the University Ulm (65/07). All surgical patients admitted to the Anaesthesiology adult ICU between August 2008 and December 2008 with severe SIRS/sepsis, septic or haemorrhagic shock on admission or developing septic or haemorrhagic shock on the ICU were included in the present study, in total, 51 cases ≥ 18 years with shock. Four patients of the hypovolaemic shock group and three patients of the septic shock group had to be excluded from further analysis, due to refusal of informed consent, stay < 24 hours on ICU, early demission to another ICU or concomitant cardiogenic shock. Thus, in total, blood samples of 44 critically ill shock patients were included for analysis of BKV deoxyribonucleic acid (DNA), 18 in the septic shock group and 26 in the hemorrhagic shock group (http://ClinicalTrials.gov ID: NCT00736827). Blood samples were taken before, during and after shock: on the first days of severe SIRS/sepsis; shock with a noradrenaline dose in μg/kg × min of > 0 and ≤ 0.1 > 2 h, > 0.1 and ≤ 1.0 > 2 h, > 1.0 > 2 h, or > 1.0 + adrenaline > 2 h; after septic shock; before demission from the ICU or before death.
Diagnosis of sepsis, acute kidney injury (AKI) and severity of organ dysfunctions and of disease
Sepsis and septic shock were defined using the 2003 SCCM/ESICM/ACCP/ATS/SIS sepsis definitions . Shock was defined as hypotension despite adequate volume resuscitation, a systolic blood pressure of ≤ 90 mmHg, or the need of vasopressors to keep blood pressure ≥ 90 mmHg.
Acute kidney injury (AKI) was defined by the Acute Kidney Injury Network criteria , which are based on acute alterations in serum creatinine or urine output. Severity of organ dysfunctions was evaluated daily by the SOFA score (Sequential Organ Failure Assessment) , and of sepsis by the 2003 sepsis definitions .
Blood samples were taken from arterial lines of the patients at the ICU. Samples were immediately centrifuged and plasma was stored at -20°C until testing.
Detection of BKV DNA by real-time PCR
The presence of BKV DNA was tested via real-time polymerase chain reaction (PCR) . DNA was extracted from 200 μl of the plasma samples using the High Pure viral nucleic acid kit (Roche, Mannheim, Germany) according to the instructions of the manufacturer. The efficiency of the nucleic acid purification was controlled by co-extraction and subsequent amplification of Herpes virus saimiri DNA. The BKV real-time PCR was performed in a final volume of 20 μl consisting of 5 μl of DNA, primers, probe at a final concentration of 200 nM each, and 1 × Quantitect probe master mix (QIAGEN, Hilden, Germany). For real-time BK PCR, primer 1 with sequence (5'-3')AGCAGGCAAGGGTTCTATTACTAAAT, primer 2 with GAAGCAACAGCAGATTCTCAACA, and probe with AAGACCCTAAAGACTTTCCCTCTGATCTACACCAGTTT were used . Amplification was performed on a 7500 real-time PCR system (Applied Biosystems, Darmstadt, Germany). The cycling conditions were 50 cycles with 30 s at 95°C and 60 s at 60°C after a preheating step of 15 min at 95°C. The lower limit of quantification was 300 copies/ml. General laboratory procedures to prevent PCR contamination were strictly adhered to. One negative probe was extracted and amplified for every five samples.
Rates of BKV DNA detection in blood of consecutive patients are given. No statistical analyses were performed.
The study included 44 patients, 18 in septic shock and 26 in hemorrhagic shock.
Patient's characteristics and scores are summarized in Table 1. Median total stay in the ICU was 9 days (range 2-52) and in the hospital 30 days (range 3-117), respectively. Twenty-two patients had no acute kidney injury (AKI), and 22 AKI stages 1 to 3. 9/44 patients required renal replacement therapy (RRT).
At least two, and up to five blood samples were collected per patient. A total of 125 plasma samples was analyzed for BKV DNA. Results regarding viral load before, during and after shock are given in Table 2. In total, five blood samples of four patients were tested positive for BKV DNA. The viral load in all positive samples was low (≤4.4 × 102 copies/ml). One septic shock patient, only, revealed AKI stage 2 at maximum and BKV viremia. None of the four BKV positive patients needed RRT (Tables 1 and 2). None of the nine patients with RRT was tested positive for BKV viral DNA.
The main result of the present study is that BKV viremia was rarely found in critically ill surgical patients with shock and with low viral load, only.
Due to the small sample size, conclusions have to be drawn with caution. We focused on BKV viremia, since BKV nephropathy with renal failure has been reported to be strongly associated with viral load in patient's blood . All patients with a low viral load (< 1.9 × 105 copies/ml) had a stable renal function without treatment . Thus, our results with low viral load (≤4.4 × 102 copies/ml) and only one BK positive patient revealing AKI stage 2, are in agreement with these data .
In immunosuppressed patients, onset of viruria and viremia occurred 40.5 days (range: 0-415 days) and 60 days (range: 18-276 days) post renal transplantation . Median onset of BKV replication in urine by detecting decoy cells was estimated at 16 weeks (range: 2-69 weeks), of viral DNA in blood at 23 weeks (range: 4-73 weeks), and of BK polyoma virus associated nephropathy (PyVAN) at 28 weeks (range: 8-86 weeks) following renal transplantation . Thus, only patient 5 (Table 2) was in the range, BKV nephropathy might have been detected, underlying the reported range of 56 up to 602 days . Thus, our observation period may have been too short to detect BK nephropathy.
In the present study, diagnosis of AKI was based on current clinical definitions , which do not reflect the underlying pathogenetic mechanisms of BK-PyVAN, diagnosed by renal biopsy and histologically on tubular-interstitial nephropathy .
In renal transplant recipients, a high BK viral load in plasma (> 10000 copies/ml) for more than three weeks suggests that BK-PyVAN is highly likely, i.e. "presumptive BK-PyVAN" . In our four patients with BK viremia, viral load was low and did not exceed three weeks, making BK-PyVAN very unlikely. Decoy cells and viral DNA in urine are used for screening purposes and response to therapeutic interventions for BK-PyVAN. Due to low viral load in blood in our patients, these screening methods might have detected more cases of BK reactivation, however, with even minor relevance for the clinician since large nucleus decoy cells do not necessarily point to the development of BK-PyVAN.
Studies in non-renal solid organ transplant recipients have shown variable prevalence of BK viruria and scarcely BK viremia during the first post-transplant year and in allograft recipients with deteriorating renal function, and presence of BKV in urine or blood was not associated with worse renal function [1, 19–24]. Thus, a second, organ-specific hit, such as donor-recipient human leukocyte antigen mismatch, appeared to be necessary for the final development of BK viremia and/or nephropathy . A missing second hit and an adequate humoral immune response  in our four BK positive patients may have contributed to avoidance of high level BK viremia and presumptive or manifest BK-PyVAN.
In conclusion, BKV viremia in critically ill surgical haemorrhagic and septic shock patients appears to be rare and associated with low BK viremia, only. Thus, it is very unlikely that BKV viremia results in nephropathy later on in these patients.
Puliyanda DP, Amet N, Dhawan A, Hilo L, Radha RK, Bunnapradist S, Czer L, Martin P, Jordan S, Toyoda M: Isolated heart and liver transplant recipients are at low risk for polyomavirus BKV nephropathy. Clin Transplant. 2006, 20 (3): 289-294. 10.1111/j.1399-0012.2005.00480.x.
Hariharan S: BK virus nephritis after renal transplantation. Kidney Int. 2006, 69 (4): 655-662. 10.1038/sj.ki.5000040.
Ling PD, Lednicky JA, Keitel WA, Poston DG, White ZS, Peng R, Liu Z, Mehta SK, Pierson DL, Rooney CM, et al: The dynamics of herpesvirus and polyomavirus reactivation and shedding in healthy adults: a 14-month longitudinal study. J Infect Dis. 2003, 187 (10): 1571-1580. 10.1086/374739.
Kaneko T, Moriyama T, Tsubakihara Y, Horio M, Imai E: Prevalence of human polyoma virus (BK virus and JC virus) infection in patients with chronic renal disease. Clin Exp Nephrol. 2005, 9 (2): 132-137. 10.1007/s10157-005-0348-9.
Heininger A, Jahn G, Engel C, Notheisen T, Unertl K, Hamprecht K: Human cytomegalovirus infections in nonimmunosuppressed critically ill patients. Crit Care Med. 2001, 29 (3): 541-547. 10.1097/00003246-200103000-00012.
von Muller L, Klemm A, Weiss M, Schneider M, Suger-Wiedeck H, Durmus N, Hampl W, Mertens T: Active cytomegalovirus infection in patients with septic shock. Emerg Infect Dis. 2006, 12 (10): 1517-1522. 10.3201/eid1210.060411.
Luyt CE, Combes A, Deback C, Aubriot-Lorton MH, Nieszkowska A, Trouillet JL, Capron F, Agut H, Gibert C, Chastre J: Herpes simplex virus lung infection in patients undergoing prolonged mechanical ventilation. Am J Respir Crit Care Med. 2007, 175 (9): 935-942. 10.1164/rccm.200609-1322OC.
Limaye AP, Kirby KA, Rubenfeld GD, Leisenring WM, Bulger EM, Neff MJ, Gibran NS, Huang ML, Santo Hayes TK, Corey L, et al: Cytomegalovirus reactivation in critically ill immunocompetent patients. JAMA. 2008, 300 (4): 413-422. 10.1001/jama.300.4.413.
Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL, Ramsay G: 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Intensive Care Med. 2003, 29 (4): 530-538.
Mehta RL, Kellum JA, Shah SV, Molitoris BA, Ronco C, Warnock DG, Levin A: Acute Kidney Injury Network: report of an initiative to improve outcomes in acute kidney injury. Crit Care. 2007, 11 (2): R31-10.1186/cc5713.
Vincent JL, de Mendonca A, Cantraine F, Moreno R, Takala J, Suter PM, Sprung CL, Colardyn F, Blecher S: Use of the SOFA score to assess the incidence of organ dysfunction/failure in intensive care units: results of a multicenter, prospective study. Working group on "sepsis-related problems" of the European Society of Intensive Care Medicine. Crit Care Med. 1998, 26 (11): 1793-1800. 10.1097/00003246-199811000-00016.
Moreno RP, Metnitz PG, Almeida E, Jordan B, Bauer P, Campos RA, Iapichino G, Edbrooke D, Capuzzo M, Le Gall JR: SAPS 3--From evaluation of the patient to evaluation of the intensive care unit. Part 2: Development of a prognostic model for hospital mortality at ICU admission. Intensive Care Med. 2005, 31 (10): 1345-1355. 10.1007/s00134-005-2763-5.
Knaus WA, Draper EA, Wagner DP, Zimmerman JE: APACHE II: a severity of disease classification system. Crit Care Med. 1985, 13 (10): 818-829. 10.1097/00003246-198510000-00009.
Hirsch HH, Mohaupt M, Klimkait T: Prospective monitoring of BK virus load after discontinuing sirolimus treatment in a renal transplant patient with BK virus nephropathy. J Infect Dis. 2001, 184 (11): 1494-1495. 10.1086/324425.
Brennan DC, Agha I, Bohl DL, Schnitzler MA, Hardinger KL, Lockwood M, Torrence S, Schuessler R, Roby T, Gaudreault-Keener M, et al: Incidence of BK with tacrolimus versus cyclosporine and impact of preemptive immunosuppression reduction. Am J Transplant. 2005, 5 (3): 582-594. 10.1111/j.1600-6143.2005.00742.x.
Hirsch HH, Knowles W, Dickenmann M, Passweg J, Klimkait T, Mihatsch MJ, Steiger J: Prospective study of polyomavirus type BK replication and nephropathy in renal-transplant recipients. N Engl J Med. 2002, 347 (7): 488-496. 10.1056/NEJMoa020439.
Hurault de Ligny B, Francois A, Lobbedez T, Comoz F, Etienne I, El Haggan W, Pujo M, Godin M, Ryckelynck JP: Clinical aspects of human polyomaviruses in renal transplantation. Presse Med. 2003, 32 (14): 659-666.
Hirsch HH, Brennan DC, Drachenberg CB, Ginevri F, Gordon J, Limaye AP, Mihatsch MJ, Nickeleit V, Ramos E, Randhawa P, et al: Polyomavirus-associated nephropathy in renal transplantation: interdisciplinary analyses and recommendations. Transplantation. 2005, 79 (10): 1277-1286. 10.1097/01.TP.0000156165.83160.09.
Barton TD, Blumberg EA, Doyle A, Ahya VN, Ferrenberg JM, Brozena SC, Limaye AP: A prospective cross-sectional study of BK virus infection in non-renal solid organ transplant recipients with chronic renal dysfunction. Transpl Infect Dis. 2006, 8 (2): 102-107. 10.1111/j.1399-3062.2006.00155.x.
Thomas LD, Vilchez RA, White ZS, Zanwar P, Milstone AP, Butel JS, Dummer S: A prospective longitudinal study of polyomavirus shedding in lung-transplant recipients. J Infect Dis. 2007, 195 (3): 442-449. 10.1086/510625.
Salama M, Boudville N, Speers D, Jeffrey GP, Ferrari P: Decline in native kidney function in liver transplant recipients is not associated with BK virus infection. Liver Transpl. 2008, 14 (12): 1787-1792. 10.1002/lt.21627.
Doucette KE, Pang XL, Jackson K, Burton I, Carbonneau M, Cockfield S, Preiksaitis JK: Prospective monitoring of BK polyomavirus infection early posttransplantation in nonrenal solid organ transplant recipients. Transplantation. 2008, 85 (12): 1733-1736. 10.1097/TP.0b013e3181722ead.
Loeches B, Valerio M, Perez M, Banares R, Ledesma J, Fogeda M, Salcedo M, Rincon D, Bouza E, Munoz P: BK virus in liver transplant recipients: a prospective study. Transplantation Proc. 2009, 41 (3): 1033-1037. 10.1016/j.transproceed.2009.02.021.
Pendse SS, Vadivel N, Ramos E, Mudge GH, Von Visger T, Fang JC, Chandraker A: BK viral reactivation in cardiac transplant patients: evidence for a double-hit hypothesis. J Heart Lung Transplant. 2006, 25 (7): 814-819. 10.1016/j.healun.2006.03.008.
Hariharan S, Cohen EP, Vasudev B, Orentas R, Viscidi RP, Kakela J, DuChateau B: BK virus-specific antibodies and BKV DNA in renal transplant recipients with BKV nephritis. Am J Transplant. 2005, 5 (11): 2719-2724. 10.1111/j.1600-6143.2005.01080.x.
The authors declare that they have no competing interests.
MN, BW, EMS and MW participated in study conception, study design, data analysis, interpretation and drafting of the manuscript. MN, MH and MW participated in collection of blood samples and data acquisition. MN and BW performed the detection of BKV DNA via real-time polymerase chain reaction (rt-PCR). All authors read and approved the final manuscript.
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Nass, M., Weissbrich, B., Huber, M. et al. BK viremia in critically ill surgical patients with hemorrhagic or septic shock. BMC Res Notes 5, 100 (2012). https://doi.org/10.1186/1756-0500-5-100
- Acute kidney injury
- Critically ill
- BK virus