Figure 4
From: RASSF1C modulates the expression of a stem cell renewal gene, PIWIL1
Western blot analysis of lung primary epithelial cells CRL-9482 (A), A549 (B) lung cancer cells, and NCI-H1299 (C) lung cancer cells stably transduced with MLV-backbone (CRL9482-BB, A549-BB, and NCI-BB) and (MLV-HA-RASSF1C: CRL9482-1C, A549-1C, and NCI-1C) treated with 1 μg/ml doxycycline for 24 hr. Anti-PIWIL1 antibody detected higher levels of PIWIL1 protein in CRL-9482, A549, NCI-H1299 and cells over-expressing RASSF1C confirming up-regulation of PIWIL1 expression by RASSF1C.