Figure 7
Overexpression of FaZnF increases transcription of selected salt stress genes. A) RT qPCR analysis of Festuca arundinacea calli using ΔΔCt quantification with GAPDH as the housekeeping gene. Values are normalized to control sample values (non-overexpressing calli) in order to represent relative expression changes. Error bars represent the SEM (Standard Error of the Mean). B) RT qPCR analysis of Festuca arundinacea calli using Ct quantification with UBC as the housekeeping gene. Values are normalized to control sample values (non-overexpressing calli) in order to represent relative expression changes. Error bars represent the SEM. Note that both housekeeping gene normalizations maintained the same trends in fold induction.