- Short Report
- Open Access
Reduced hatchability of Anopheles gambiae s.s eggs in presence of third instar larvae
© Gotifrid et al.; licensee BioMed Central Ltd. 2014
Received: 25 January 2013
Accepted: 4 April 2014
Published: 11 April 2014
We investigated the hatchability rates of freshly laid Anopheles gambiae s.s. eggs in presence of third instars larvae. These experiments were conducted using 30 eggs in larval densities of 20, 60 and 100 larvae in microcosms. These experiments were designed to evaluate the eggs hatchability in habitats with late larvae instars of the same species (experimental) or no larvae at all (control). Freshly laid eggs of An.gambiae s.s. were washed in microcosms containing larvae of third instars in different three densities (20, 60 and 100) and likewise in control microcosms (without larvae). Eggs hatchability was monitored twice daily until no more first instar larvae emerged. The numbers of first instars larvae were recorded daily and lost eggs were considered preyed upon by third instars.
The findings of this study showed that egg hatchability was significantly influenced by larval density.
The findings of this study suggest that presence of larvae in habitats may significantly reduce hatchability of eggs.
Hatchability comparison of eggs between controls bowls and bowls with different larvae densities
Number of eggs
Percentage egg hatchability
% hatchability reduction
Study was conducted at Tropical Pesticides Research Institute Insectary, based in Arusha Tanzania for two months.
Adult mosquitoes rearing and eggs laying
Three days old females of An. gambiae s.s post emergence mosquitoes were fed on rabbit for 30 minutes. Blood fed females were then kept in insectary at a temperature of 27 ± 2°C, Relative humidity 78 ± 2% and light 12 L: 12D. The gravid females after 72 hrs post feeding were given a wet filter paper in a cage to act as oviposition substrate. The eggs laid were used immediately for these experiments.
Hatchability experimental set up
Experiments were set up in white microcosms having a diameter of 16.7 cm and depth of 1.7 cm. The sides of the microcosms just at the level of the water were lined with white paper to prevent the eggs from adhering to the surface of the microcosm and drying up. Freshly laid eggs on filter papers were washed in microcosms with dechlorinated water with third instar larvae in three densities of 20, 60 and 100. In the control arm, eggs were washed in microcosms without larvae and in both experiments; hatchability was monitored for three days. Hatched first instar larvae were collected and taken out of the microcosms every two hours’ time. Insectary temperature was maintained at 27 ± 2°C and relative humidity was 78 ± 2%. Thirty freshly laid eggs of An. gambiae s.s. were introduced in each microcosm in all three densities of An.gambiae s.s larvae. Each experiment had six replicates for each density and control.
Data were analyzed using SPSS 17.0 (SPSS Inc., Chicago, IL). Comparison of the mean number of hatched eggs was compared by ANOVA between the larvae densities in treatments and control. The significance level for the means of the three densities of 20, 60 and 100 were separated by Tukey HSD test.
The study was approved by Tropical Pesticides Research Institute (TPRI), Proposal review and ethical committee. The use of rabbit for feeding mosquitoes was approved as a daily routine permission in mosquito colony maintenance at TPRI.
This study has shown that the existence of the third instar larvae in breeding sites affect egg hatchability but also survivorship of the newly hatched first instars. More studies have to be done in semi field environment to determine egg hatchability in more complex environments and investigation of larva produced chemical factors (cuticle exudates) that play the role of emergence inhibitors of conspecific eggs is on progress.
Authors wish to thank Mr. Adrian Massawe and Ms. Ester Lyatuu for mosquitoes rearing, experimental set up and eggs hatchability monitoring.
- Ponnusamy L, Xu N, Nojima S, Wesson DM, Schal C, Apperson CS: Identification of bacteria and bacteria-associated chemical cues that mediate oviposition site preferences by Aedes aegypti. Proc Natl Acad Sci. 2008, 105: 9262-9267. 10.1073/pnas.0802505105.PubMedPubMed CentralView ArticleGoogle Scholar
- Arav D, Blaustein L: Effects of pool depth and risk of predation on oviposition habitat selection by temporary pool dipterans. J Med Entomol. 2006, 43: 493-497. 10.1603/0022-2585(2006)43[493:EOPDAR]2.0.CO;2.PubMedView ArticleGoogle Scholar
- Blaustein L, Blaustein J, Chase J: Chemical detection of the predator Notonecta irrorata by ovipositing Culex mosquitoes. J Vector Ecol. 2005, 30: 299-301.PubMedGoogle Scholar
- Blaustein L, Kiflawi M, Eitam A, Mangel M, Cohen JE: Oviposition habitat selection in response to risk of predation in temporary pools: mode of detection and consistency across experimental venue. Oecologia. 2004, 138: 300-305. 10.1007/s00442-003-1398-x.PubMedView ArticleGoogle Scholar
- Kweka EJ, Zhou G, Lee MC, Gilbreath TM, Mosha F, Munga S, Githeko AK, Yan G: Evaluation of two methods of estimating larval habitat productivity in western Kenya highlands. Parasit Vectors. 2011, 4: 110-10.1186/1756-3305-4-110.PubMedPubMed CentralView ArticleGoogle Scholar
- Kweka EJ, Zhou G, Munga S, Lee MC, Atieli HE, Nyindo M, Githeko AK, Yan G: Anopheline larval habitats seasonality and species distribution: a prerequisite for effective targeted larval habitats control programmes. PLoS One. 2012, 7: e52084-10.1371/journal.pone.0052084.PubMedPubMed CentralView ArticleGoogle Scholar
- McCrae AW: Oviposition by African malaria vector mosquitoes. II. Effects of site tone, water type and conspecific immatures on target selection by freshwater Anopheles gambiae Giles, sensu lato. Ann Trop Med Parasitol. 1984, 78: 307-318.PubMedGoogle Scholar
- Lyons CL, Coetzee M, Chown SL: Stable and fluctuating temperature effects on the development rate and survival of two malaria vectors, Anopheles arabiensis and Anopheles funestus. Parasit Vectors. 2013, 6: 104-10.1186/1756-3305-6-104.PubMedPubMed CentralView ArticleGoogle Scholar
- Lindh JM, Kannaste A, Knols BG, Faye I, Borg-Karlson AK: Oviposition responses of Anopheles gambiae s.s. (Diptera: Culicidae) and identification of volatiles from bacteria-containing solutions. J Med Entomol. 2008, 45: 1039-1049. 10.1603/0022-2585(2008)45[1039:OROAGS]2.0.CO;2.PubMedView ArticleGoogle Scholar
- Sumba LA, Ogbunugafor CB, Deng AL, Hassanali A: Regulation of oviposition in Anopheles gambiae s.s.: role of inter- and intra-specific signals. J Chem Ecol. 2008, 34: 1430-1436. 10.1007/s10886-008-9549-5.PubMedView ArticleGoogle Scholar
- Zahiri N, Rau ME: Oviposition attraction and repellency of Aedes aegypti ( Diptera: Culicidae) to waters from conspecific larvae subjected to crowding, confinement, starvation, or infection. J Med Entomol. 1998, 35: 782-787.PubMedView ArticleGoogle Scholar
- Munga S, Vulule J, Kweka EJ: Response of Anopheles gambiae s.l. (Diptera: Culicidae) to larval habitat age in western Kenya highlands. Parasit Vectors. 2013, 6: 13-10.1186/1756-3305-6-13.PubMedPubMed CentralView ArticleGoogle Scholar
- Munga S, Minakawa N, Zhou G, Barrack OO, Githeko AK, Yan G: Effects of larval competitors and predators on oviposition site selection of Anopheles gambiae sensu stricto. J Med Entomol. 2006, 43: 221-224. 10.1603/0022-2585(2006)043[0221:EOLCAP]2.0.CO;2.PubMedView ArticleGoogle Scholar
- Munga S, Minakawa N, Zhou G, Barrack OO, Githeko AK, Yan G: Oviposition site preference and egg hatchability of Anopheles gambiae: effects of land cover types. J Med Entomol. 2005, 42: 993-997. 10.1603/0022-2585(2005)042[0993:OSPAEH]2.0.CO;2.PubMedGoogle Scholar
- Koenraadt CJ, Takken W: Cannibalism and predation among larvae of the Anopheles gambiae complex. Med Vet Entomol. 2003, 17: 61-66. 10.1046/j.1365-2915.2003.00409.x.PubMedView ArticleGoogle Scholar
- Muturi EJ, Kim CH, Jacob B, Murphy S, Novak RJ: Interspecies predation between Anopheles gambiae s.s. and Culex quinquefasciatus larvae. J Med Entomol. 2010, 47: 287-290. 10.1603/ME09085.PubMedPubMed CentralView ArticleGoogle Scholar
- Gimnig JE, Ombok M, Otieno S, Kaufman MG, Vulule JM, Walker ED: Density-dependent development of Anopheles gambiae (Diptera: Culicidae) larvae in artificial habitats. J Med Entomol. 2002, 39: 162-172. 10.1603/0022-2585-39.1.162.PubMedView ArticleGoogle Scholar
- Kweka EJ, Zhou G, Beilhe LB, Dixit A, Afrane Y, Gilbreath TM, Munga S, Nyindo M, Githeko AK, Yan G: Effects of co-habitation between Anopheles gambiae s.s. and Culex quinquefasciatus aquatic stages on life history traits. Parasit Vectors. 2012, 5: 33-10.1186/1756-3305-5-33.PubMedPubMed CentralView ArticleGoogle Scholar
- Hatano E, Kunert G, Michaud JP, Weisser WW: Chemical cues mediating aphid location by natural enemies. Eur J Entomol. 2008, 105: 797-806. 10.14411/eje.2008.106.View ArticleGoogle Scholar
- Santos ND, de Moura KS, Napoleao TH, Santos GK, Coelho LC, Navarro DM, Paiva PM: Oviposition-stimulant and ovicidal activities of Moringa oleifera lectin on Aedes aegypti. PLoS One. 2012, 7: e44840-10.1371/journal.pone.0044840.PubMedPubMed CentralView ArticleGoogle Scholar
- Fagbenro-Beyioku AF, Oyibo WA, Anuforom BC: Disinfectant/antiparasitic activities of Jatropha curcas. East Afr Med J. 1998, 75: 508-511.PubMedGoogle Scholar
- Spencer M, Blaustein L, Cohen JE: Oviposition habitat selection by mosquitoes (Culiseta longireolata) and consequences for population size. Ecology. 2002, 83: 669-679. 10.1890/0012-9658(2002)083[0669:OHSBMC]2.0.CO;2.View ArticleGoogle Scholar
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