Figure 12

SDS-polyacrylamide gel electrophoresis showing proteome changes during interaction of A. terreus with potato tuber slice by 96 h. (A) Crude 20 μg proteins was separated by 1-D on a 15% gel; lane 1, 2 and 3 are molecular weight marker, potato tuber proteins without A. terreus and potato tuber inoculated with A. terreus, respectively. 2-D images of crude proteins showing region of interest: Isoelectric focusing (IEF) was performed with 140 μg of proteins on an IPG strip (pH 4–7, 7 cm) followed by separation on a 15% gel; (B) inoculated tuber and (C) unchallenged tuber. (D) 1-D profile for identified induced proteins during colonization of potato tuber slice by 96 h and all gels were stained with Coomassie Blue R250.