Characterization of microsatellite markers developed from Prosopis rubriflora and Prosopis ruscifolia (Leguminosae - Mimosoideae), legume species that are used as models for genetic diversity studies in Chaquenian areas under anthropization in South America

Table 3 Markers developed for Prosopis ruscifolia

Marker	N_a			H_o		H_e		PIC	Null alleles		HW ( P-value)
Marker	T	FRC	ECD	FRC	ECD	FRC	ECD	PIC	FRC	ECD	FRC	ECD
Prsc1	7	4	7	0.600^a	0.783	0.566^a	0.828	0.701	0.000	0.020	0.916	0.009
Prsc2	8	7	5	0.480	0.565	0.553	0.761	0.658	0.032	0.114	0.034	0.00^c
Prsc3	3	3	3	0.360^a	0.348	0.344^a	0.456	0.348	0.000	0.048	0.674	0.073
Prsc4	5	4	3	0.040	0.043	0.321	0.275	0.289	0.247^b	0.221^b	0.000^c	0.000^c
Prsc5	6	4	5	0.440^a	0.273	0.378^a	0.654	0.484	0.000	0.218^b	1.000	0,000^c
Prsc6	8	4	8	0.320	0.696	0.653	0.801	0.703	0.196	0.068	0.001^c	0.087
Prsc7	9	6	7	0.440	0.565	0.727	0.779	0.755	0.167	0.081	0.001^c	0.000^c
Prsc8	7	4	7	0.320	0.174	0.577	0.789	0.656	0.165	0.338^b	0.008	0.000^c
Prsc9	5	3	3	0.240	0.130	0.280	0.559	0.430	0.040	0.267^b	0.484	0.000^c
Prsc10	7	5	5	0.286	0.227	0.633	0.758	0.670	0.212^b	0.294^b	0.000^c	0.000^c
Prsc11	17	8	12	0.560	0.652	0.845	0.884	0.883	0.157	0.127	0.003	0.000^c
Prsc12	5	4	5	0.600	0.591	0.569	0.707	0.589	0.000	0.075	0.592	0.013

FRC - Fazenda Retiro Conceição, ECD - Estação do Carandazal, N_a - Number of alleles, H_o - Observed heterozygosity, H_e - Expected heterozygosity, PIC - Polymorphism information content, P-values of Hardy-Weinberg (HW) equilibrium (P-value > 0.0041 after Bonferroni correction). ^aPopulations where the values of H_o were higher than those of H_e; ^bPossible null alleles (null frequency < 0.20); ^cDeparture from HW equilibrium was observed.

ISSN: 1756-0500