- Research article
- Open Access
Disinfection by hydrogen peroxide nebulization increases susceptibility to avian pathogenic Escherichia coli
© Oosterik et al. 2015
- Received: 26 November 2014
- Accepted: 12 August 2015
- Published: 27 August 2015
Avian pathogenic Escherichia coli (APEC) are the major cause of economic losses in the poultry industry worldwide. Traditionally, antibiotics are used to treat and prevent colibacillosis in broilers. Due to resistance development other ways of preventing/treating the disease have to be found. Therefore during this study the nebulization of low concentrations of hydrogen peroxide (H2O2) was tested in the presence of chickens to lower pathogenicity of APEC.
Significantly higher total lesion scores and higher E. coli concentrations were found in the spleen of chickens exposed to 2 % H2O2 compared to those exposed to 1 % H2O2 and control chickens which had been exposed to nebulization with distilled water. Higher total lesions scores and E. coli concentrations in the spleen were found in chickens exposed to 1 % H2O2 in comparison to control chickens (not significant).
H2O2 is rendering animals more prone to APEC infection contraindicating H2O2 nebulization in the presence of chickens.
- Hydrogen peroxide
- Avian pathogenic Escherichia coli
Avian pathogenic Escherichia coli (APEC) causes colibacillosis in chickens of all ages, leading yearly to major losses to the poultry industry [1, 2]. Antibiotics have been used for a long time to treat and prevent diseases in chickens; with the consequence of antibiotic resistance development posing a huge threat to the public health worldwide  and transfer of virulence and resistance genes to other bacteria [4–6]. Sanitation and cleaning is a first way to reduce infection pressure , but is normally performed between different flocks in the barn to prevent flock to flock transmission. The information about the use of disinfectants in the presence of animals to lower pathogenicity of APEC is limited. On conditions safe to the animals, nebulization of hydrogen peroxide (H2O2) in the presence of chickens may possibly lower pathogenicity of APEC, since (pathogenic) E. coli is normally present in high concentrations in the barn in the form of faeces-contaminated dust [1, 2, 8]. Predisposing factors, such as viral live vaccines, render chickens more prone to APEC infection [9, 10]. Several studies on H2O2 toxicity have been performed on rats, mice, rabbits and dogs but there is no documentation on such studies in chickens. As toxicity is depending on many factors like exposure time, number of exposures and concentration  the goal of this study was to test if H2O2 nebulization in the presence of chickens can lower pathogenicity of APEC.
The animal experiment was approved by the ethical committee of the KU Leuven (Permit Number: P176/2011) and conducted in strict accordance with the Federation of European Laboratory Animal Science Associations guidelines.
Fifteen one-day-old chickens (Ross; Belgabroed NV, Merksplas, Belgium) were housed in a disinfected barn and provided with water and feed ad libitum. At 4 weeks of age, the chickens were orally vaccinated against Newcastle disease (106 mean egg infective dose; MSD Animal Health, Boxmeer, the Netherlands) to render them subsequently more susceptible to APEC challenge. Three days post vaccination (dpv), the chickens were transferred and equally divided over three disinfected isolators (dimensions: 1.5 × 1.0 × 1.0 m, volume ≈ 1.6 m3). Three and 4 dpv they were aerogenically infected with a virulent APEC strain CH2  (10 ml of 1010 colony forming units/ml (cfu/ml)) with the help of a compressor (N 811 KN.18; KNF Neuberger, Aarselaar, Belgium) and nebulizer (Cirrus 2; Intersurgical, Uden, the Netherlands). The chickens were exposed to the aerosols for 60 min. One day thereafter or 5 dpv they were exposed to 10 ml of different H2O2 concentrations (EcoClearProx®, ABT Belgium BVBA, Affligem, Belgium) by nebulization for 60 min [Group 1—2 % H2O2; Group 2—1 % H2O2; Group 3—distilled water (DW)].
Eleven dpv all surviving chickens were euthanized by cervical dislocation for clinical necropsy. Macroscopic lesions were scored according to Vandemaele et al. .
From two or three chickens per group (chickens that had macroscopic lesions) the spleen was aseptically removed and homogenized in sterile phosphate buffered saline (PBS) (1 g of tissue in 1 ml PBS) as described before [13, 14]. Ten-fold dilutions were plated on MacConkey agar (Oxoid CM0115; Erembodegem, Belgium) and incubated at 37 °C for 24 h, after which bacterial numbers (cfu/g) were determined.
Results were analyzed with SAS software version 9.4 (SAS Institute Cary, North Carolina, USA) and R . The sum of the total lesion score and the lesion scores per organ were calculated for the three groups. To evaluate the hypothesis of a higher total lesion score in infected chickens treated with DW than H2O2, the total lesion scores were analyzed by means of an ordinal logistic regression model. Results of ordinal logistic regression are presented as the odds ratio of having a high lesion score. Average bacterial concentrations were compared using the Kruskal–Wallis Test for significant differences between multiple groups and the Wilcoxon Rank Sum Test for differences between each pair of groups. P ≤ 0.05 was considered significant.
Mortality and lesion scores of infected/treated chickens
Sum of lesion scores
2 % H2O2
1 % H2O2
This worsening effect after H2O2 nebulization is probably due to the caustic effect of H2O2 radicals on (ciliated) epithelial cells, such as the case for Newcastle disease virus infection (or vaccination with virulent vaccines) in one-day-old chickens , making it easier for APEC to cross the upper respiratory tract causing colibacillosis. The presented results give a good indication that nebulization of H2O2 (higher than 1 %) in the presence of chickens is not advisable. As toxicity was also shown to be dependent on exposure time and number of exposures (Anonymous 2003), these parameters should also be taken into consideration, during future studies. H2O2 concentrations lower than 1 % can, however, be used in an experimental respiratory infection model to predispose the chickens to APEC infection.
H2O2 nebulization in the presence of chickens in order to lower pathogenicity of APEC is not advisable, since it makes chickens more susceptible to APEC infection.
LHO participated in the design of the study, carried out the study, and drafted the manuscript. HTN participated in the design of the study and participated in the animal infection study. SJ performed the statistical analysis. PB and BMG conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
We thank Jessica Tsonos and Tom Luyten for their assistance. This study was funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment (contract RT 09/8 APECON) and by CODA-CERVA (contract APEC-BED).
Compliance with ethical guidelines
Competing interests The author(s) declare that they have no competing interests.
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- Barnes HJ, Nolan LK, Vaillantcourt JP. Colibacillosis. In: Saif YM, editor. Diseases of poultry. 12th ed. Ames: Blackwell Publishing; 2008. p. 691–732.Google Scholar
- Dziva F, Stevens MP. Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts. Avian Pathol. 2008;37(4):355–66.View ArticlePubMedGoogle Scholar
- Singer RS, Hofacre CL. Potential impacts of antibiotic use in poultry production. Avian Dis. 2006;50(2):161–72.View ArticlePubMedGoogle Scholar
- Ewers C, Li G, Wilking H, Kiessling S, Alt K, Antáo EM, Laturnus C, Diehl I, Glodde S, Homeier T, et al. Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: how closely related are they? Int J Med Microbiol. 2007;297(3):163–76.View ArticlePubMedGoogle Scholar
- Johnson JR, Murray AC, Gajewski A, Sullivan M, Snippes P, Kuskowski MA, Smith KE. Isolation and molecular characterization of nalidixic acid-resistant extraintestinal pathogenic Escherichia coli from retail chicken products. Antimicrob Agents Chemother. 2003;47(7):2161–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Johnson JR, Delavari P, O’Bryan TT, Smith KE, Tatini S. Contamination of retail foods, particularly turkey, from community markets (Minnesota, 1999-2000) with antimicrobial-resistant and extraintestinal pathogenic Escherichia coli. Foodborne Pathog Dis. 2005;2(1):38–49.View ArticlePubMedGoogle Scholar
- Gehan MG, Anwer W, Amer HM, IM W, Rezk A, Badawy EM. In vitro efficacy comparisons of disinfectants used in the commercial poultry farms. Int J Poult Sci. 2009;8(3):237–41.View ArticleGoogle Scholar
- Dho-Moulin M, Fairbrother JM. Avian pathogenic Escherichia coli (APEC). Vet Res. 1999;30(2–3):299–316.PubMedGoogle Scholar
- Nakamura K, Ueda H, Tanimura T, Noguchi K. Effect of mixed live vaccine (Newcastle disease and infectious bronchitis) and Mycoplasma gallisepticum on the chicken respiratory tract and on Escherichia coli infection. J Comp Pathol. 1994;111(1):33–42.View ArticlePubMedGoogle Scholar
- La Ragione RM, Woodward MJ. Virulence factors of Escherichia coli serotypes associated with avian colisepticaemia. Res Vet Sci. 2002;73(1):27–35.View ArticlePubMedGoogle Scholar
- Anonymous: European Union risk assessment report hydrogen peroxide. In: Munn SJ, Allanou R, Aschberger K, Berthault F, De Bruijn J, Musset C, O’Connor S, Pakalin S, Paya-Perez A, Pellegrini G et al, vol 38. Luxembourg: Office for Official Publications of the European Communities; 2003. p 246.Google Scholar
- Vandemaele F, Ververken C, Bleyen N, Geys J, D’Hulst C, Addwebi T, van Empel P, Goddeeris BM. Immunization with the binding domain of FimH, the adhesin of type 1 fimbriae, does not protect chickens against avian pathogenic Escherichia coli. Avian Pathol. 2005;34(3):264–72.View ArticlePubMedGoogle Scholar
- Tuntufye HN, Lebeer S, Gwakisa PS, Goddeeris BM. Identification of avian pathogenic Escherichia coli genes that are induced in vivo during infection in chickens. Appl Environ Microbiol. 2012;78(9):3343–51.View ArticlePubMedPubMed CentralGoogle Scholar
- Tsonos J, Oosterik LH, Tuntufye HN, Klumpp J, Butaye P, De Greve H, Hernalsteens J-P, Lavigne R, Goddeeris BM. A cocktail of in vitro efficient phages is not a guarantee for in vivo therapeutic results against avian colibacillosis. Vet Microbiol. 2014;171(3–4):470–9.View ArticlePubMedGoogle Scholar
- R Development Core Team: R: A language and environment for statistical computing. R Foundation for Statistical Computing. http://www.R-project.org (2015). Last visited: January 2015.
- Mast J, Nanbru C, van den Berg T, Meulemans G. Ultrastructural changes of the tracheal epithelium after vaccination of day-old chickens with the La sota strain of Newcastle disease virus. Vet Pathol Online. 2005;42(5):559–65.View ArticleGoogle Scholar