Open Access

High conductance potassium channels activation by acid exposure in rat aorta is endothelium-dependent

  • Andrea Carla Celotto1,
  • Verena Kise Capellini1,
  • Agnes Afrodite Sumarelli Albuquerque1,
  • Luciana Garros Ferreira1,
  • Ana Paula Cassiano Silveira1,
  • Tales Rubens de Nadai1 and
  • Paulo Roberto Barbosa Evora1, 2Email author
BMC Research Notes20158:462

https://doi.org/10.1186/s13104-015-1422-3

Received: 1 December 2014

Accepted: 7 September 2015

Published: 19 September 2015

Abstract

Background

We investigated, previously, the mechanism by which extracellular acidification promotes relaxation in rat thoracic aorta. These studies suggested that extracellular acidosis promotes vasodilation mediated by NO, KATP and SKCa, and maybe other K+ channels in isolated rat thoracic aorta. This study was carried out to investigate the paxilline-mediated hyperpolarization induced by acid exposure.

Results

The relaxation response to HCl-induced extracellular acidification (7.4–6.5) was measured in rat aortic rings pre-contracted with phenylephrine (PE, 10−6 M). The vascular reactivity experiments were performed in endothelium-intact and denuded rings, in the presence of paxilline (10−6 M), which is an inhibitor of high calcium conductance potassium BKCa channels. In rings with endothelium, paxilline inhibits relaxation, triggered by acidification at all pH values lower than 7.2 and had no effect on rings without endothelium, showing that the activation of BKCa is endothelium-dependent.

Conclusion

High conductance potassium channel activation induced by acid exposure is endothelium-dependent.

Keywords

Potassium channels Endothelium Paxilline Rat aortic rings

Background

We investigated, previously, the mechanism by which extracellular acidification promotes relaxation in rat thoracic aorta. The extracellular acidosis failed in inducing any changes in the vascular tone of rings pre-contracted with potassium chloride (KCl). However, it caused endothelium-dependent, and endothelium-independent, relaxations in rings pre-contracted with PE. These relaxations were inhibited by L-NAME, apamin, and glibenclamide, but not by indomethacin. These results suggested that extracellular acidosis promotes vasodilation mediated by NO, KATP and SKCa, and maybe other K+ channels in the isolated rat thoracic aorta [1]. In rings with endothelium, paxilline inhibits relaxation induced by acidification at all pH values lower than 7.2. Otherwise, paxilline had no effect on rings without endothelium, showing that the activation of BKCa is endothelium-dependent. After an extensive literature search, we assumed this data as original. Therefore, the rationale of the present study was to test a hypothesis that the relaxation induced by extracellular acidification includes the activation of high conductance BKCa potassium channels.

Methods

The experimental procedures and animal handling were reviewed and approved by the Institutional Animal Care Review Board (CETEA—Ethics Committees of Animal Experiments of the Faculty of Medicine of Ribeirão Preto—University of São Paulo). This investigation conforms with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996). Before the experiments, the rats were housed under standard laboratory conditions (12 h light/dark cycle at 21 °C), with free access to food and water. Male Wistar rats (230–280 g) were anesthetized with thiopental sodium (40 mg/kg, intraperitoneal injection), followed by a laparotomy for exsanguination via the abdominal aorta and a thoracotomy for thoracic aorta harvesting. The thoracic aorta was carefully dissected free of connective tissue and immediately immersed in Krebs. For the vascular reactivity studies, Krebs solution with the following composition (mM) was employed: NaCl—118.0, KCl—4.7, CaCl2—2.5, KH2PO4—1.2, MgSO4—1.66, glucose—11.1, NaHCO3—25.0 (pH 7.4).

The thoracic aorta was cut into rings (4–5 mm in length). The endothelium was removed from some rings by gently rubbing the intimal surface of the blood vessel with a pair of watchmaker’s forceps. This method removes the endothelium, but it does not affect the ability of the vascular smooth muscle to contract or relax. Then, these rings were placed in isolated organ baths (10 mL) filled with Krebs solution, maintained at 37 °C, and bubbled with 95 % O2/5 % CO2 (pH 7.4). Each arterial ring was suspended by two stainless steel clips placed through the lumen. One clip was anchored to the bottom of the organ bath, while the other was connected to a strain gauge for measurement of the isometric force with the aid of the Grass FT03 equipment (Grass Instrument Company, Quincy, MA, USA). Each ring was stretched to a resting tension of 1.5 g and allowed to equilibrate for 60 min. During this period, tissues were washed every 15 min. The efficacy of the procedure for endothelium removal was confirmed by the lack of relaxant effects induced by acetylcholine (10−6 M) in rings pre-contracted with PE (10−6 M—EC80). Endothelium was considered to be present when the Ach-induced relaxation was at least 80 %. Then, each ring was washed and re-equilibrated for 30 min. Aortic rings were then pre-contracted with PE (10−6 M), and pH-response curves were obtained after a stable plateau was reached. The pH-response curves allowed assessment of the relaxation response to HCl-induced (1N) extracellular acidification (7.4, 7.3, 7.2, 7.1, 7.0, 6.9, 6.8, 6.7, 6.6, 6.5). For this purpose, an electrode (Analion G2134, Ribeirão Preto, SP, Brazil) connected to a pH-meter (MS tecnopon MPA 210, Piracicaba, SP, Brazil) was immersed in the organ bath, enabling real-time analysis of the Krebs solution pH, each point of the curve was obtained by adding a sufficient volume (approximately 2–3 μL) of HCl to the preparation, in order to decrease the pH in 0.1 units. The time elapsed between consecutive additions of acid was 1–2 min, which was necessary for observation of the effect of pH-changes and achievement of the plateau. Considering that the pH curve ranged from 7.4 to 6.5, the total final volume of HCl added to the organ bath was about 30 μL. The paxilline added to the bath was 10−6 M. Preparations were kept in contact with the drugs for an incubation period of 30 min. Phenylephrine (PE) and paxilline were purchased from Sigma Chemical Company (St. Louis, MO, USA). All the drugs were prepared with distilled water.

The results are shown as mean ± SEM. In the relaxation study, the changes in vascular tension are expressed as a percent of relaxation about the maximal contraction achieved by EC80 PE-stimulated contraction, a convention that corrects interanimal variability. Statistical analysis was performed using two-way repeated-measures ANOVA and Bonferroni post-test (Prism 4.0, GraphPad Software Inc, San Diego, CA, USA). Values were considered to be statistically significant at p values less than 0.05.

Results

In rings with endothelium precontracted with PE (10−6 M), paxilline inhibits relaxation, induced by HCl acidification at all pH values lower than 7.2 (n = 6; p < 0.05) (Fig. 1).
Fig. 1

a pH-response curves for acidification in rat aortic rings with endothelium precontracted with PE (10−6 M) in the absence and presence of paxilline (10−6 M); b relaxation at pH 7.2 and pH 6.8. Data represent mean ± SEM (n = 6), ap <0.01, bp <0.001 e cp <0.05 (versus control paxilline), two-way ANOVA, Bonferroni post-test

In rings without endothelium precontracted with PE (10−6 M), paxilline did not inhibit the relaxation induced by HCl acidification at all pH values (n = 6; p < 0.05) (Fig. 2).
Fig. 2

a pH-curves in response to acidification of rat aortic rings without endothelium, precontracted with PE (10−6 M) in the absence and presence of paxilline (10−6 M). b Relaxation at pH 7.2 and pH 6.8. Data represent mean ± SEM (n = 6), two-way ANOVA, Bonferroni post-test

Discussion

Several types of K+ channels have been identified in the vascular smooth muscle. These channels had been shown to be involved in the vasodilation seen in cerebral circulation under acidosis condition. KATP and KCa are the most important potassium channels engaged in the relaxant response [26] in the presence of decreased extracellular pH. It is known that high potassium concentrations can inhibit the activity of all potassium channels [4, 7].

To confirm the involvement of this two potassium channels in acidosis-induced vasodilation, we previously tested the specific channels KATP and KCa under acidosis condition concluding that their vascular beds actions were endothelium-independent. Vasodilation in response to acidosis was completely abolished when only one of these channels was blocked. The inhibitory effect of glibenclamide or apamin on vasodilation HCl-induced, was potenciated by endothelium removal. The association between L-NAME and specific potassium channels blockers, inhibited the HCl-relaxation almost a hundred percent. [1]. This data is suggestive that hyperpolarization and NO/cGMP has some “cross-talked” mechanism. It has been shown that the vasodilator response to NO in rat middle cerebral artery is mediated by activation of KCa channels via a cGMP- independent pathway [8], a finding that is in accordance with our results about the important role of NO and KCa channel activity in acidosis.

To evaluate the involvement of BKCa, this study used paxilline [9], because although some studies have used charybdotoxin as a blocker of BKCa [2, 10], it is known that this drug is not unique to this channel [1114]. The present studies, using the blocker paxilline, showed an interesting data: the effect of acidification mediated by potassium channel type BKCa is dependent on the endothelium. However, it is prudent to consider the fact that paxilline inhibit the relaxation only in rings with endothelium, suggesting that the opening of these channels should be dependent on some endothelium-derived factor. From this point of view, the paxilline BKCa channel blocker effect, by itself, should not participate in the relaxation induced by acidification. In addition, it is possible to speculate that the endothelium role is dependent on calcium conductance, since the acidosis-induced relaxation blocked by apamin (small conductance Ca2+ -activated K + channel blocker—SKCa) was endothelium-independent, and paxilline (a high calcium conductance BKCa) was endothelium-dependent.

The present study demonstrated the contribution of BKCa for dilation evoked by decreased pHo and paxilline proved to be useful as a pharmacological tool. For example, recent study about the vasorelaxant effect of cilostazol a selective inhibitor of type III phosphodiesterase (PDE3), was not affected by removal of the endothelium and the results were quite similar to the decreased pHo effect. Application of a nitric oxide synthase inhibitor and a small conductance Ca2+-activated K+ (SKCa) channel inhibitor did not affect cilostazol-induced vasorelaxation, which was endothelium-independent and only blocked by paxilline [15].

In conclusion, high conductance potassium channel (BKCa) activation induced by acid exposure is endothelium-dependent (Fig. 3).
Fig. 3

Overall scheme possible inhibition of BKCa channels by paxilline

Abbreviations

PE: 

phenylephrine

BKCa

calcium-activated potassium channels

KCl: 

potassium chloride

L-NAME: 

L-NG-nitroarginine methyl ester

NO: 

nitric oxide

KATP

ATP-sensitive potassium channel

SKCa

calcium-activated SK potassium channel

cGMP: 

cyclic guanosine monophosphate

Declarations

Authors’ contributions

ACC and VKC participated in the design, performed the statistical analysis and drafted the manuscript. PRBE participated in the design, helped to draft the manuscript and made suggestions for the analyzes. AASA, LGF, TRN and APCS conceived the study and participated in its design and coordination. All authors read and approved the final manuscript.

Acknowledgements

We are thankful to Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Fundação de Apoio ao Ensino, Pesquisa e Assistência do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (FAEPA/HCFMRP/USP) for financial support.

Compliance with ethical guidelines

Competing interests The authors declare that they have no competing interests.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Department of Surgery and Anatomy, Laboratory of Endothelial Function, School of Medicine, University of São Paulo
(2)

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Copyright

© Celotto et al. 2015

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