|
Locus
|
Primer sequence (5′–3′)
|
Primer label
|
Repeat motif
|
Ta (°C)
|
n
|
HWE
|
K
|
Ho
|
He
|
GenBank accession no.
|
|---|
|
Coco6
|
F: *AACCAAGCTGTCAAAGGAGA
|
VIC
|
(ATCT)13
|
60/50
|
20
|
0.396
|
8
|
0.55
|
0.54
|
KT288258
|
|
R: TTTCCTGGTGCGGAGAAGTA
| | | | | | | | | |
|
Coco12
|
F: *GAGGCTTTCAGCGATATGGG
|
6FAM
|
(AGAT)7
|
60/50
|
18
|
0.173
|
5
|
0.72
|
0.72
|
KT288259
|
|
R: GCTGCACTTGCCTGTTGTTTA
| | | | | | | | | |
|
Coco30
|
F: *GTGCAAACCACTCCAGAAT
|
6FAM
|
(GGAT)16
|
60/50
|
20
|
0.910
|
6
|
0.70
|
0.80
|
KT288260
|
|
R: GCTGTCACCAGTGGCTTTAAT
| | | | | | | | | |
|
Coco31
|
F: *TGGCTACTTACCCGGAATCTC
|
NED
|
(ATGT)8
|
60/50
|
20
|
0.470
|
3
|
0.25
|
0.30
|
KT288261
|
|
R: CTTCTCTACCGCCCATCACA
| | | | | | | | | |
|
Coco32
|
F: ATGCAAATCTCCGTGTATCA
|
NED
|
(ATCT)9
|
60/50
|
20
|
0.422
|
8
|
0.80
|
0.80
|
KT288262
|
|
R: *CCTCCCTTTGTCCTTTCTGG
| | | | | | | | | |
|
Coco36
|
F: CCTTCTGCGCTGTGTTCAAA
|
NED
|
(CTT)8
|
60/50
|
20
|
0.008
|
5
|
0.40
|
0.71
|
KT288263
|
|
R: *CTGAGGTGCAAGCTTTCCTG
| | | | | | | | | |
|
Coco45
|
F: TGACCAACACAGAGCAGATATT
|
6FAM
|
(GT)11
|
60/50
|
19
|
0.353
|
3
|
0.53
|
0.54
|
KT288264
|
|
R: *GGAACCTCTGAGTCCAAA
| | | | | | | | | |
|
Coco50
|
F: *ATCCCATCAAAGGTCCACG
|
VIC
|
(AC)13
|
60/50
|
20
|
0.157
|
5
|
0.50
|
0.64
|
KT288265
|
|
R: CCACGACAGCTGATGAGAGA
| | | | | | | | | |
- All loci were in Hardy–Weinberg and linkage equilibrium after Bonferroni correction for multiple tests
-
Asterisks show location of CAG tag for each primer pair, T
a
annealing temperatures for touchdown protocol for each locus, n number of individuals genotyped, K number of alleles, H
o
observed heterozygosity, H
e
expected heterozygosity
