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Table 2 Comparison of residual ATP detected in a soiled colonoscope instrument channel evaluated using various sample collection methods

From: Simulated-use validation of a sponge ATP method for determining the adequacy of manual cleaning of endoscope channels

Channel sample collection methoda

RLUsb (standard deviation)

Protein μg/cm2 (standard deviation)

Sponge directc

9304.2 (1028.6)

Not applicable

Sponge eluted (20 mL sample)

2511.4 (1142.1)

0.54 (0.14)

Flush only (20 mL sample)

2850.4 (1261.6)

26.66 (5.98)

  1. aData for each test parameter represents the average of five replicate endoscopes tested (N = 5)
  2. bATP was reported as relative light units (RLUs)
  3. cThe range of RLUS for the sponge direct collection method was 7709–9999 with three of the five replicates having RLU values of 9999 (i.e. maximum RLU value read by the luminometer). This 9999 maximum RLU value was used for the purposes of calculating the average and standard deviation recognizing that it was not an exact RLU value