Table 1 Details of Sium latifolium samples used for testing of the microsatellite primer sets and assessing the loci

Individual from which the microsatellite sequences were isolated

 I50

Wickhampton Marshes, Norfolk

TG 43535 05018

Samples used for PCR temperature gradient testing

 G15

Sutton Fen, Norfolk

TG 36511 22999

 I08

Wickhampton Marshes, Norfolk

TG 43433 04160

Six unrelated individuals used initially to test for polymorphism

 B12

Tophill Low, East Riding of Yorkshire

TA 07754 49673

 C12

Ouse Washes, Cambridgeshire

TL 49433 89016

 D15

Romney Marsh, Kent

TQ 97837 31120

 E33

Southlake Moor, Somerset

ST 36427 30272

 F20

Cantley Marsh, Norfolk

TG 37352 03459

 G10

Sutton Fen, Norfolk

TG 36881 23345

24 individuals from one population

 I01

Wickhampton Marshes, Norfolk

TG 43381 04180

 I02

Wickhampton Marshes, Norfolk

TG 43318 04021

 I03

Wickhampton Marshes, Norfolk

TG 43532 04032

 I04

Wickhampton Marshes, Norfolk

TG 43193 03934

 I05

Wickhampton Marshes, Norfolk

TG 43408 03171

 I06

Wickhampton Marshes, Norfolk

TG 43471 04113

 I07

Wickhampton Marshes, Norfolk

TG 43441 04132

 I10

Wickhampton Marshes, Norfolk

TG 43921 04759

 I11

Wickhampton Marshes, Norfolk

TG 44163 04634

 I13

Wickhampton Marshes, Norfolk

TG 44177 04656

 I15

Wickhampton Marshes, Norfolk

TG 43295 03952

 I16

Wickhampton Marshes, Norfolk

TG 43325 04226

 I17

Wickhampton Marshes, Norfolk

TG 43316 04050

 I18

Wickhampton Marshes, Norfolk

TG 43291 03947

 I19

Wickhampton Marshes, Norfolk

TG 43291 04157

 I20

Wickhampton Marshes, Norfolk

TG 43252 03931

 I22

Wickhampton Marshes, Norfolk

TG 43285 04125

 I24

Wickhampton Marshes, Norfolk

TG 43295 03961

 I25

Wickhampton Marshes, Norfolk

TG 44129 04558

 I26

Wickhampton Marshes, Norfolk

TG 43288 04151

 I27

Wickhampton Marshes, Norfolk

TG 43299 04101

 I28

Wickhampton Marshes, Norfolk

TG 43250 03931

 I29

Wickhampton Marshes, Norfolk

TG 43663 04256

 I30

Wickhampton Marshes, Norfolk

TG 44131 04556