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Magnitude of the Cytomegalovirus infection among pregnant women attending antenatal clinics in the city of Mwanza, Tanzania
BMC Research Notes volume 10, Article number: 489 (2017)
Despite, Cytomegalovirus (CMV) infection being associated with a potential risk to the fetus, there is limited data from Tanzania and many other developing countries regarding the epidemiology and the impact of CMV infections. This cross-sectional study was conducted between December 2014 and June 2015 among pregnant women attending antenatal clinics in the city of Mwanza, Tanzania to investigate the magnitude and associated factors of CMV infection.
The specific CMV IgM and IgG antibodies were detected using indirect enzyme linked immunosorbent assay (ELISA). Demographic and clinical data were collected using pre-tested data collection tool. Data were analysed using STATA version 13.
A total of 261 pregnant women with median age of 20 (IQR 19–25) years and mean gestation age of 17 ± 7.8 weeks were enrolled. The seroprevalence of CMV IgG antibodies was 193(73.9%; 95% CI 67.2–79.6) while that of CMV IgM antibodies was 0.4%. As the age increased by one unit the IgG seroprevalence was found to increase by 0.3% (95% CI 0.13–0.47, p = 0.001) whereas the risk of being IgG positive increased by 24%. On multivariable logistic regression analysis only urban residence (OR 6.329, 95% CI 2.885–13.887, p < 0.001) was found to independently predict CMV IgG seropositivity. Regarding the outcomes of previous pregnancies the history of miscarriage independently predicted IgG seropositivity (OR 5.6, 95% CI 1.29–24.178, p = 0.021). The IgM seropositive woman had fatal outcome of the term delivery of the baby with microcephaly and spinal-bifida.
Cytomegalovirus seroprevalence among pregnant women residing in urban areas of Mwanza city, Tanzania is high and is associated with poor pregnancy outcomes. There is a need to emphasize routine screening of CMV in order to establish the impact of CMV during pregnancy.
Primary Cytomegalovirus (CMV) infection during pregnancy poses a serious threat to the fetus and newborn. CMV is ubiquitous in nature and can be transmitted through direct contact or vertically from pregnant women to the fetus . In contrast to other congenital infections such as rubella virus , CMV infection can be primary, re-infection or reactivation . Primary maternal infection is associated with 30–40% risk of fetal infections, and 20–25% risk of adverse pregnancy outcomes . Intrauterine infections can cause fetal death in approximately 10% of fetuses . Approximately 15% of newborns with congenital infections develop neurologic disorders such as sensory-neural hearing loss, mental retardation and delayed milestone .
The seroprevalence of CMV among pregnant women has been found to be high ; with highest seroprevalence being reported in Turkey, Qatar, Saudi Arabia, Taiwan and sub-Saharan Africa [8,9,10,11,12,13,14,15,16]. In Tanzania, there is limited information about the magnitude of CMV among pregnant women with no single report from Mwanza city despite having high prevalence of the congenital defects with unidentified causes [17, 18]. Considering its potential in causing congenital defects; it is important to understand the magnitude and associated factors of CMV among pregnant women. Therefore, this study for the first time in Tanzania has established that there is high seroprevalence of CMV infection among pregnant women and is associated with adverse pregnancy outcomes; the information that may be important to reinforce its control measures in the developing countries.
A cross sectional hospital based study was conducted from December 2014 to July 2015 among pregnant women attending Karume (Rural) and Makongoro (Urban) antenatal clinics. About 4–5 ml of blood was collected using plain vacutainer tubes (Becton, Dickinson and Company, Nairobi, Kenya) and transported to the Bugando Medical Centre laboratory. Sera were separated and stored at −80 °C until processing.
Socio-demographic data were collected using pre-tested questionnaire. Data collected included; age, parity, gestation age, the outcome of the previous pregnancies, education level, residence, and socio-economic status. Good social economic status was defined as being educated with sustainable income or having reliable income generating activities .
Detection of the specific Cytomegalovirus (CMV) IgM and IgG antibodies were done using commercial indirect enzyme-linked immunosorbent assay(PishtazTeb, Tehran, Iran) according to the manufacturer’s instructions using automated ELISA system (ChemWell® 2910-Awareness Technology Inc. USA). The ELISA assays have been found to have sensitivity and specificity of > 95% [20,21,22,23]. The system was calibrated using provided standards and controls before any sample testing was done. In addition HIV testing was done to all samples based on the national rapid test algorithm .
Data management and analysis
Data were entered in the computer using Microsoft Office Excel 2007 and analyzed using the STATA version 11 (College Station, Texas, USA). Continuous variables were summarized as median with interquartile range while categorical variables were summarized as proportions. Univariable and multivariable logistic regression analysis was done to investigate factors associated with CMV seropositivity. For factors associated with IgG seropositivity, all factors found to be significant on univariable analysis were subjected to the multivariable logistic regression analysis while for the relation between the outcome of the previous pregnancies and CMV IgG seropositivity all factors were fitted in the multivariable model. Odds ratio (OR) and 95% confidence interval were noted and a p value of < 0.05 was considered significant.
The median age of the 261 enrolled women was 20 (IQR 19–25) years with mean gestation age of 17 ± 7.8 weeks. One hundred and five (40.2%), 115 (44.06%) and 41 (15.7%) were in the first, second and third trimester respectively. As defined by the Mwanza municipal city council; the majority of women 169 (64.8%) were from urban areas. Also it was observed that the majority of women 192 (73.9%) had poor socioeconomic status. Out of 261 pregnant women, 22 (8.4%) were found to be HIV positive.
Seroprevalence of CMV and associated factors
Overall seroprevalence of CMV IgM specific antibodies was 1 (0.4%) while for IgG specific antibodies the seroprevalence was 193 (73.9%, 95% CI 68.5–79.2). On Wilcoxon Ranksum–Mann–Whitney test; the median age of IgG seropositive women was significantly high compared to that of IgG seronegative women [22 (IQR 19–27) vs. 20 (IQR 18–20), p < 0.001]. As the age increases by one year the seroprevalence was found to increase by 0.3% (95% CI 0.13–0.47, p = 0.001) while the risk of being CMV IgG positive increased by 24% (Fig. 1). On univariable logistic regression analysis, the IgG seropositivity was found to decrease significantly as gestation age increases (OR 0.1, 95% CI 1.04–2.23, p < 0.001). The mean gestation age of IgG seropositive women was significantly lower than the mean gestation age of IgG seronegative women (15.1 ± 6.8 vs. 22 ± 7.9, p = 0.001).
Increased in age, being multiparous and low socioeconomic status were significantly found to be associated with CMV IgG seroprevalence (p < 0.01) Table 1. On multivariable logistic regression analysis only urban residence was found to be an independent predictor of CMV IgG seropositivity among pregnant women (OR: 6.34; 95% CI 2.9–13.9, p < 0.001).
Outcome of previous pregnancies and current pregnancy for IgM positive case
Based on the outcomes of the previous pregnancies; all women with previous history of still birth and history of giving birth to a baby with congenital malformations were CMV IgG sero-positive. In addition, women with history of miscarriage had significantly high IgG seroprevalence than those with no history of miscarriage (93.3% vs. 71%, p = 0.021) Table 2. The history of miscarriage remained to be significant factor associated with IgG seropositivity on the multivariable logistic regression analysis. IgM positive participant had term delivery of a baby with microcephaly and spinal-bifida; the baby died within 2 h of delivery.
Understanding the magnitude of the Cytomegalovirus (CMV) infections among pregnant women is essential for exploring its epidemiology and control measures. The CMV has been found to be among the common cause of hearing loss and mental retardation among many other defects in congenitally infected children [25,26,27]. However; the epidemiology of CMV infections and its impact is limited in the many developing countries including Tanzania. As observed in previous studies in developing countries [8, 12, 13, 16], a significant proportion of pregnant women were found to be CMV IgG seropositive. The results from this study and other studies in the developing countries call for a joint effort to control CMV infections considering its potential effect in causing congenital defects and poor pregnancy outcomes. The CMV management guidelines provide a framework for the diagnosis and management of suspected CMV infections with the emphasis on the importance of screening and follow up using ultrasound . Women with primary infections during pregnancy should be informed about a 30–40% risk of the intrauterine transmission and fetal infections, and a risk of 20–25% of developing sequelae if the fetus is infected. The use of passive immunization (CMV-hyper-immune globulin) has been found to be beneficial in preventing congenital anomalies .
As per previous observations in other studies [13, 29], the acute CMV infection as indicated by positive CMV IgM antibodies was found to be very low in the current study. This could be explained by the fact that the accuracy of IgM antibodies in predicting primary infection is not reliable as the IgM may persist for several months after primary infection as well as in cases of reactivation or re-infection . In most cases there is a need to perform IgG avidity test as an alternative to provide the status of active infection [31,32,33]. Due to the limited resources this this could not be done in our study.
Despite the fact that the trend on reactivation and re-infection were not established in this study; the high IgG seropositivity is alarming which calls for the need to screen these women for active infections for the proper management provision. The importance of these results is further supported by the fact that all women with history of stillbirth and child with congenital malformations were IgG seropositive. In addition, the IgM seropositive woman in the present study had poor pregnancy outcome. Further studies on the impact of CMV on poor pregnancy outcomes are highly recommended in the developing countries.
Residing in urban areas independently predicted CMV IgG seropositivity which is inconsistent to what was reported earlier . The majority of population in the city of Mwanza reside in highly populated squatters with close contacts which favors transmission of airborne diseases. Also, on univariable analysis, increase in age was significantly found to be associated with IgG seropositivity, this confirms what was observed in the previous studies [3, 34,35,36,37,38,39]. Being the childhood illness and endemic in most of the sub-Saharan African countries; there is possibility that most of these women were either exposed during childhood and as the age increases are more likely to be infected. Though borderline significant in multivariable analysis, low socioeconomic status was found to predict CMV IgG seropositivity which is similar to what was observed in the previous studies [34, 40]. This could be explained by poor hygienic conditions which have been found to perpetuate the cycle of CMV transmission in the developing countries . The decrease in CMV IgG seropositivity with gestation age observed in the current study might be due to pregnancy hemodilution .
HIV infected women were more likely to be CMV IgG seropositive than HIV negative; however the difference was not statistically significant. Of note was that a total of 57.8% of the pregnant women had no HIV results by the time of the recruitment which is against the WHO recommendations. WHO recommends that all the pregnant women should be counseled and tested for HIV at the first antenatal visit. There is a need to improve antenatal services in our setting to ensure all women are counseled and tested for HIV.
In this study one of the limitations is failure to detect CMV DNA that indicates active shedding of CMV which is an important risk factor for vertical transmission.
Cytomegalovirus seroprevalence among pregnant women residing in urban areas of Mwanza city is significantly high and might be causing poor pregnancy outcomes such as miscarriage, congenital anomalies and stillbirth. This calls for the need to screen CMV infection during antenatal visits in order to provide appropriate management for the purpose of minimizing associated adverse outcomes. Further studies to investigate the outcome of pregnancy in relation to acute CMV infection are highly needed in the developing countries.
enzyme linked immunosorbent assay
Cannon MJ. Congenital cytomegalovirus (CMV) epidemiology and awareness. J Clin Virol. 2009;46:S6–10.
Mirambo MM, Majigo M, Aboud S, Groß U, Mshana SE. Serological makers of rubella infection in Africa in the pre vaccination era: a systematic review. BMC Res Notes. 2015;8(1):716.
Wang C, Zhang X, Bialek S, Cannon MJ. Attribution of congenital cytomegalovirus infection to primary versus non-primary maternal infection. Clin Infect Dis. 2011;52(2):e11–3.
Yinon Y, Farine D, Yudin MH, Gagnon R, Hudon L, Basso M, Bos H, Delisle M-F, Menticoglou S, Mundle W. Cytomegalovirus infection in pregnancy. J Obstet Gynaecol Canada. 2010;32(4):348–54.
Boppana SB, Pass RF, Britt WJ, Stagno S, Alford CA. Symptomatic congenital cytomegalovirus infection: neonatal morbidity and mortality. Pediatr Infect Dis J. 1992;11(2):93–8.
Fowler KB, Stagno S, Pass RF. Maternal age and congenital cytomegalovirus infection: screening of two diverse newborn populations, 1980–1990. J Infect Dis. 1993;168(3):552–6.
Mocarski E, Shenk T, Pass R. Chapter 69: Cytomegaloviruses. In: Knipe DM, Howley PM, editors. Fields virology. Philadelphia: Lippincott Williams & Wilkins; 2007.
Spano L, Gatti J, Nascimento J, Leite J. Prevalence of human cytomegalovirus infection in pregnant and non-pregnant women. J Infect. 2004;48(3):213–20.
Yamamoto AY, Mussi-Pinhata MM, Pinto PCG, Figueiredo LTM, Jorge SM. Congenital cytomegalovirus infection in preterm and full-term newborn infants from a population with a high seroprevalence rate. Pediatr Infect Dis J. 2001;20(2):188–92.
Yamamoto A, Castellucci R, Aragon D, Mussi-Pinhata M. Early high CMV seroprevalence in pregnant women from a population with a high rate of congenital infection. Epidemiol Infect. 2013;141(10):2187–91.
Chen MH, Chen PC, Jeng SF, Hsieh CJ, Su FC, Liao HF, Su YN, Lin SJ, Hsieh WS. High perinatal seroprevalence of cytomegalovirus in northern Taiwan. J Paediatr Child Health. 2008;44(4):166–9.
Uyar Y, Balci A, Akcali A, Cabar C. Prevalence of rubella and cytomegalovirus antibodies among pregnant women in northern Turkey. New Microbiol. 2008;31(4):451–5.
Tamer GS, Dundar D, Caliskan E. Seroprevalence of Toxoplasma gondii, rubella and cytomegalovirus among pregnant women in western region of Turkey. Clin Invest Med. 2009;32(1):43–7.
Abu-Madi MA, Behnke JM, Dabritz HA. Toxoplasma gondii seropositivity and co-infection with TORCH pathogens in high-risk patients from Qatar. Am J Trop Med Hyg. 2010;82(4):626–33.
Ghazi HO, Telmesani AM, Mahomed MF. TORCH agents in pregnant Saudi women. Med Princ Pract. 2002;11(4):180–2.
Rodier M, Berthonneau J, Bourgoin A, Giraudeau G, Agius G, Burucoa C, Hekpazo A, Jacquemin J. Seroprevalences of Toxoplasma, malaria, rubella, cytomegalovirus, HIV and treponemal infections among pregnant women in Cotonou, Republic of Benin. Acta Trop. 1995;59(4):271–7.
Mashuda F, Zuechner A, Challya P, Kidenya BR, Manyama M. Pattern and factors associated with congenital anomalies among young infants admitted at Bugando medical centre, Mwanza, Tanzania. BMC Res Notes. 2014;7(1):195.
Mhalu F, Haukenes G. Prevalence of cytomegalovirus antibody in pregnant women, AIDS patients and STD patients in Dar es Salaam. AIDS. 1990;4(12):1294.
Taylor S, Yu D. The importance of socio-economic status in determining educational achievement in South Africa. Unpublished working paper (Economics). Stellenbosch: Stellenbosch University; 2009.
Souza VAUFd, Sumita LM, Otsubo MES, Takei K, Pannuti CS: Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report. Revista do Instituto de Medicina Tropical de São Paulo. 1995; 37(4):357–359.
Field P, Ho D, Cunningham A. Evaluation of rubella immune status by three commercial enzyme-linked immunosorbent assays. J Clin Microbiol. 1988;26(5):990–4.
Chernesky M, Wyman L, Mahony J, Castriciano S, Unger J, Safford J, Metzel P. Clinical evaluation of the sensitivity and specificity of a commercially available enzyme immunoassay for detection of rubella virus-specific immunoglobulin M. J Clin Microbiol. 1984;20(3):400–4.
Schwarz TF, Jäger G, Gilch S. Comparison of seven commercial tests for the detection of parvovirus B19-specific IgM. Zentralblatt für Bakteriologie. 1997;285(4):525–30.
Lyamuya EF, Aboud S, Urassa WK, Sufi J, Mbwana J, Ndugulile F, Massambu C. Evaluation of simple rapid HIV assays and development of national rapid HIV test algorithms in Dar es Salaam, Tanzania. BMC Infect Dis. 2009;9(1):1.
Hyde TB, Schmid DS, Cannon MJ. Cytomegalovirus seroconversion rates and risk factors: implications for congenital CMV. Rev Med Virol. 2010;20(5):311–26.
Kenneson A, Cannon MJ. Review and meta-analysis of the epidemiology of congenital cytomegalovirus (CMV) infection. Rev Med Virol. 2007;17(4):253–76.
Manicklal S, Emery VC, Lazzarotto T, Boppana SB, Gupta RK. The “silent” global burden of congenital cytomegalovirus. Clin Microbiol Rev. 2013;26(1):86–102.
Nigro G, Adler SP, La Torre R, Best AM. Passive immunization during pregnancy for congenital cytomegalovirus infection. N Engl J Med. 2005;353(13):1350–62.
Drew RJ, Stapleton P, Abu H, Healy E, Ferguson W, De Gascun C, O’Gorman J, Eogan M. pregnancy outcomes of mothers with detectable CMV-specific IgM antibodies: a three-year review in a large irish tertiary referral maternity hospital. Infect Dis Obstet Gynecol. 2015;2015:5.
Lazzarotto T, Ripalti A, Bergamini G, Battista M, Spezzacatena P, Campanini F, Pradelli P, Varani S, Gabrielli L, Maine G. Development of a new cytomegalovirus (CMV) immunoglobulin M (IgM) immunoblot for detection of CMV-specific IgM. J Clin Microbiol. 1998;36(11):3337–41.
Grangeot-Keros L, Mayaux MJ, Lebon P, Freymuth F, Eugene G, Stricker R, Dussaix E. Value of cytomegalovirus (CMV) IgG avidity index for the diagnosis of primary CMV infection in pregnant women. J Infect Dis. 1997;175(4):944–6.
Lazzarotto T, Spezzacatena P, Pradelli P, Abate DA, Varani S, Landini MP. Avidity of immunoglobulin G directed against human cytomegalovirus during primary and secondary infections in immunocompetent and immunocompromised subjects. Clin Diagn Lab Immunol. 1997;4(4):469–73.
Lazzarotto T, Varani S, Spezzacatena P, Gabrielli L, Pradelli P, Guerra B, Landini MP. Maternal IgG avidity and IgM detected by blot as diagnostic tools to identify pregnant women at risk of transmitting cytomegalovirus. Viral Immunol. 2000;13(1):137–41.
Jindal N, Aggarwal A. A pilot seroepidemiological study of cytomegalovirus infection in women of child bearing age. Indian J Med Microbiol. 2005;23(1):34.
Ahlfors K, IVARSSON SA, Johnsson T, Svanberg L. Primary and secondary maternal cytomegalovirus infections and their relation to congenital infection. Acta Paediatr. 1982;71(1):109–13.
Mathur A, Jindal I, Chaturvedi U. A serological study of cytomegalovirus infection at Lucknow. Indian J Med Res. 1981;73:678–81.
Venkitaraman AR, SEIGNEURIN JM, LENOIR GM, JACOB JT. Infections due to the human herpes-viruses in Southern India: a seroepidemiological survey. Int J Epidemiol. 1986;15(4):561–6.
Gambarotto K, Ranger-Rogez S, Aubard Y, Piver P, Duffetelle B, Delpeyroux C, Roussanne M, Nicot T, Denis F. Primary cytomegalovirus infection and pregnant women: epidemiological study on 1100 women at Limoges. Pathol Biol. 1997;45(6):453–61.
Gratacap-Cavallier B, Bosson J, Morand P, Dutertre N, Chanzy B, Jouk P, Vandekerckhove C, Cart-Lamy P, Seigneurin J. Cytomegalovirus seroprevalence in French pregnant women: parity and place of birth as major predictive factors. Eur J Epidemiol. 1998;14(2):147–52.
Bate SL, Dollard SC, Cannon MJ. Cytomegalovirus seroprevalence in the United States: the national health and nutrition examination surveys, 1988–2004. Clin Infect Dis. 2010;50(11):1439–47.
de Jong MD, Galasso GJ, Gazzard B, Griffiths PD, Jabs DA, Kern ER, Spector SA. Summary of the II international symposium on cytomegalovirus. Antiviral Res. 1998;39(3):141–62.
Eskild A, Jeansson S, Hagen J, Jenum P, Skrondal A. Herpes simplex virus type-2 antibodies in pregnant women: the impact of the stage of pregnancy. Epidemiol Infect. 2000;125(03):685–92.
Mirambo M, Chibwe E, Mushi M, Majigo M, Mshana S. Cytomegalovirus, parvovirus B19 and rubella co-infection among pregnant women attending antenatal clinics in Mwanza City: the need to be considered in Tanzanian Antenatal Care Package. Epidemiology (Sunnyvale). 2016.doi:10.4172/2161-1165.1000230.
MMM, AK and SEM participated in the design of the study. MMM & EC participated in the collection of specimens and clinical data. MMM and EC performed serological tests. MMM and SEM analysed and interpreted the data. MMM wrote the first draft of the manuscript. All authors read and approved the final manuscript.
The authors would like to acknowledge the technical support provided by Mr Vitus Silago, Mr. Mukama, Ms Carol, Mr. Benard Okamo and all staff at Karume, Makongoro antenatal clinics and KAMANGA Medics.
Authors declare that they have no competing interests.
Availability of data and materials
All data have been included in the manuscript, however part of the data have been published in Mirambo et al. .
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Ethics approval and consent to participate
The protocol for conducting the study was approved by the Joint Catholic University of Health and Allied Sciences/Bugando Medical Centre (CUHAS/BMC) research ethics and review committee (CREC) with Certificate No: CREC/095/2015. Permission was sought from hospital/clinic administration before commencement of the study. Protocol and the importance of the study were explained. Written informed consent was obtained from each participant prior enrolment to the study.
This study was supported by research Grant from CUHAS to EC.
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Chibwe, E., Mirambo, M.M., Kihunrwa, A. et al. Magnitude of the Cytomegalovirus infection among pregnant women attending antenatal clinics in the city of Mwanza, Tanzania. BMC Res Notes 10, 489 (2017). https://doi.org/10.1186/s13104-017-2813-4
- Low socioeconomic status
- Pregnant women