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Phytochemical and pharmacological investigation of Spiraea chamaedryfolia: a contribution to the chemotaxonomy of Spiraea genus

  • Tivadar Kiss1, 2,
  • Kristóf Bence Cank1,
  • Orsolya Orbán-Gyapai1,
  • Erika Liktor-Busa1,
  • Zoltán Péter Zomborszki1, 2,
  • Santa Rutkovska3,
  • Irēna Pučka3,
  • Anikó Németh4 and
  • Dezső Csupor1, 2Email authorView ORCID ID profile
BMC Research Notes201710:762

Received: 18 July 2017

Accepted: 28 November 2017

Published: 21 December 2017



Diterpene alkaloids are secondary plant metabolites and chemotaxonomical markers with a strong biological activity. These compounds are characteristic for the Ranunculaceae family, while their occurrence in other taxa is rare. Several species of the Spiraea genus (Rosaceae) are examples of this rarity. Screening Spiraea species for alkaloid content is a chemotaxonomical approach to clarify the classification and phylogeny of the genus. Novel pharmacological findings make further investigations of Spiraea diterpene alkaloids promising.


Seven Spiraea species were screened for diterpene alkaloids. Phytochemical and pharmacological investigations were performed on Spiraea chamaedryfolia, the species found to contain diterpene alkaloids. Its alkaloid-rich fractions were found to exert a remarkable xanthine-oxidase inhibitory activity and a moderate antibacterial activity. The alkaloid distribution within the root was clarified by microscopic techniques.


PhytochemistryAlkaloids Spiraea AntibacterialXanthine-oxidaseChemotaxonomy


Plant metabolism, driven by photosynthesis, provides a huge number and a wide variety of natural products. These compounds are of great importance for their beneficial biological activities in humans. The investigation for specific plant metabolites is also a useful tool for the clarification of taxonomical uncertainties.

Diterpene alkaloids are secondary metabolites belonging to pseudoalkaloids [1]. This group of molecules includes numerous compounds with diverse skeletons and substitution patterns. These compounds can be classified according to the number of carbon atoms in the skeleton as bisnor-(C18), nor-(C19) and diterpene (C20) alkaloids. Aconitum, Delphinium and Consolida genera (Ranunculaceae) are known to be characterized by the presence of diterpene alkaloids. Although such alkaloids have also been reported from some Inula (Asteraceae), Garrya (Garryaceae), Erythrophleum (Fabaceae) and Spiraea (Rosaceae) species [2, 3], the occurrence of diterpene alkaloids in these taxa is sporadic. Since diterpene alkaloids are considered as chemotaxonomic markers [4], their presence in species other than those belonging to the Ranunculaceae family might have an important role in plant taxonomy.

The Spiraea genus, comprising approximately 100 species, belongs to the Rosaceae family. Phytochemical contents of 28 Spiraea taxa have been extensively studied. Mono-, di-, sesqui- and triterpenes have been isolated besides flavonoids, lignans, neolignans and other phenylpropane derivatives. Interestingly, only 9 of the investigated taxa were found to contain diterpene alkaloids (S. formosana Hayata, S. fritschiana var. parvifolia Liou, S. japonica L.f., S. japonica var. acuta Yu, S. japonica var. fortunei (Planchon) Rehder, S. japonica var. glabra (Regel) Koidz, S. japonica var. incisa Yu, S. japonica var. ovalifolia Zuo, S. japonica var. stellaris). All of the reported 65 diterpene alkaloids bear hetisine- and atisine-type C20 basic skeletons (Additional file 1: Spiraea diterpene alkaloids). Although only marginal ethnomedicinal use of Spiraea species has been documented in North-America and Asia, pharmacological studies have reported noteworthy activities of Spiraea extracts and isolated compounds [5].

The recent classification and clarification of Spiraea phylogeny is based mainly on molecular analyses [69]. The phytochemical analysis is also considered as a useful tool to support plant classification.

Phytochemical studies on Spiraea genus are promising, because of their possible utilization as source of pharmacons. On the other hand, screening of this genus for diterpene alkaloid content may contribute to the clarification of Spiraea phylogeny. These considerations motivated our research, aiming to improve the current phytochemical knowledge on Spiraea species.

Main text

Materials and methods

Plant material

Seven Spiraea species were analysed. S. crenata L. (SZTE-FG 850) and S. salicifolia L. (SZTE-FG 851) were collected and identified by Gusztáv Jakab (Szent István University, Budapest, Hungary) in Hungary (Sepsibükszád and Alsórákos, Hungary). S. nipponica Maxim (SZTE-FG 852), S. x vanhouttei (Briot) Zabel (SZTE-FG 853) and S. x billardii hort. ex K. Koch (SZTE-FG 854) were collected and identified by Anikó Németh (Botanical Garden of University of Szeged, Szeged, Hungary). S. media Schmidt. (DAU 0 31 147 009) and Spiraea chamaedryfolia L. (DAU 0 31 145 023) were harvested in Daugavpils (Latvia), and identification was performed by Santa Rutkovska (University of Daugavpils, Latvia). Voucher specimens were deposited at the herbarium of the Department of Pharmacognosy of the University of Szeged and at that of the University of Daugavpils. Herb and root of the plant material were separated, dried and stored at room temperature until processing.

Extraction and identification of the alkaloid content

Dried and crushed herb materials were extracted consequently with methanol (MeOH), chloroform (CHCl3) and 2% aqueous HCl, by ultrasonication at room temperature (Fig. 1). The applied drug-solvent ratio was 1:5 in each case. The drug was dried before each extraction phase. Moistening with 5% aqueous NaOH solvent was applied prior to extraction with chloroform.
Fig. 1

Alkaloid contents and pharmacological activities of S. chamaedryfolia fractions

The methanol extract was acidified with 2% aqueous HCl and was then extracted with chloroform. Fraction M1 was obtained by collecting and evaporating the organic phase. The pH of the aqueous phase was rendered to alkaline (pH 12) with 5% aqueous NaOH and was then extracted with chloroform. The chloroform phase yielded fraction M2.

The chloroform extract was further extracted with 2% aqueous HCl. The organic phase was evaporated and used as fraction L1. The pH of the aqueous phase was made alkaline and extracted with chloroform. The organic phase was evaporated to yield fraction L2.

The acidic extract was subjected to solvent–solvent partitioning with chloroform, after adjusting the pH to alkaline. The dry residue of the organic phase was labelled as S1. The pH of the aqueous phase was rendered to acidic with 2% aqueous HCl and was then extracted with chloroform. The organic phase was evaporated to produce fraction S2.

Fractions were screened for alkaloid content by thin layer chromatography (TLC), carried out at room temperature on silica gel (SiO2 60 F254, Merck 1.05554.0001) and toluene/acetone/ethanol/cc.NH3 70:50:18:4.5 was applied as mobile phase. Detection was performed in two steps: (1) dry plates were sprayed with Dragendorff’s reagent; and (2) after drying, the plates were sprayed again with 5% aqueous NaNO2. The alkaloids appeared as permanent brown spots.

Screening for antibacterial activity

Plant extracts were tested for antibacterial activity using the following microorganisms as test strains in the screening assays: 3 different Gram-positive strains, namely Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 29213), and Streptococcus pneumoniae (ATCC 49619) plus one Gram-negative strain, namely Moraxella catarrhalis (ATCC 25238). In addition, the multi-resistant strain, methicillin-resistant S. aureus (MRSA, ATCC 43300) was used to test whether the fractions have a specific antibacterial effect on a strain of high public health priority. The test organisms were cultured on standard Mueller–Hinton agar plates or Columbia agar + 5% sheep blood (COS) plates (bioMérieux) at 37 °C. The bacterial cultures were maintained in their appropriate plates at 4 °C throughout the experiment and were used as stock cultures.

Antibacterial activities of our plant extracts were evaluated by the disc-diffusion method. The bacterial isolates for screening assay were prepared by picking single colony from 24 h old plates and it was suspended in sterile, isotonic saline solution (5 mL) to reach 0.5 McFarland standard of optical turbidity, resulting in a suspension containing approximately 1–2 × 108 CFU/mL. The bacterial suspension was spread on appropriate sterile plates using a sterile cotton swab. Sterile filter paper discs (6 mm of diameter) were loaded with the extracts, using 20 μL of dried extracts redissolved in a mixture of ethanol and water (40/60 v/v) at a concentration of 50 mg/mL. After drying, these loaded filter paper discs were placed on the plates containing the bacterial suspensions. Paper discs impregnated with 20 µL of pure solvent were used as a negative control. The plates were then incubated at 37 °C for 24 h under aerobic conditions. Diameters of the inhibition zones produced by the plant extracts were measured and recorded (as the diameter of the inhibition zone plus the diameter of the disc) at 24 h.

Xanthine oxidase assay

The method is based on a continuous spectrophotometric rate determination: the absorbance of xanthine oxidase (XO) enzyme induced uric acid production from xanthine was measured at 290 nm for 3 min. The enzyme-inhibitory effect of our plant extracts was determined on the basis of the decrease in uric acid production. Reagents used included: 50 mM potassium buffer, pH 7.5 with 1 M KOH, 0.15 mM xanthine solution, pH 7.5, prepared using xanthine, XO enzyme solution 0.2 Units/mL prepared using XO. The test solutions applied included: S. chamaedryfolia fractions 12 g/mL, 600 µg/mL diluted in DMSO solution. The final reaction mixture of 300 µL well contained: 100 µL xanthine, 150 µL buffer and 50 µL XO for enzyme-activity. Allopurinol was dissolved in DMSO and used as positive control (100% inhibition was considered at 10 μg/mL concentration of allopurinol). The reaction mixture for inhibition: 100 µL xanthine, 140 µL buffer, 10 µL test and 50 µL XO.

Microscopical analysis

Specimens of the plant material were softened by ultrasonication in hot water for 1 h. Unembedded material was sectioned on a sledge microtome producing sections of 100 μm thickness. Observations were carried out on unstained sections. For histological characterisation 1% aqueous toluidine blue was used, and Dragendorff’s reagent was applied for alkaloid localisation. Transverse sections were mounted with water/glycerol 1:1. The sections were observed under light microscope and photographic images were captured using a digital camera.


Phytochemical screening revealed alkaloid content in S. chamaedryfolia roots, while all the other six Spiraea species were alkaloid-free. The solvent–solvent partitioning of methanolic, acidic and alkaline extracts of S. chamaedryfolia yielded alkaloid-rich ethyl acetate (EtOAc), chloroform and methanol fractions (Fig. 1). The most apolar fraction prepared with n-hexane (hex) was alkaloid-free. The attempt to isolate diterpene alkaloids have failed due to the low stability of the compounds.

The fractions were screened for in vitro antibacterial and xanthine oxidase inhibitory activity. The ethyl acetate fraction was found to be the most potent xanthine oxidase inhibitor, exerting over 70% of inhibition compared to allopurinol (Fig. 1 and Table 1).
Table 1

Antibacterial and xanthine oxidase inhibitory activities of S. chamaedryfolia fractions


Bacillus subtilis

Staphylococcus aureus

Streptococcus pneumoniae

Moraxella catarrhalis

Staphylococcus aureus MRSA

XO inhibition %





Fractions with activity () and fractions with no activity (). (EtOAc ethyl acetate, MeOH methanol)

Three fractions were found to exert antibacterial activity against S. aureus (ATCC 29213), B. subtilis (ATCC 6633), S. pneumoniae (ATCC 49619), and M. catarrhalis (ATCC 25238), while one fraction exerted antibacterial activity against methicillin-resistant S. aureus (MRSA) (ATCC 43300) (Fig. 1 and Table 1).

Examining the transverse section of the root of S. chamaedryfolia, structures characteristic of secondary root were observed (Fig. 2). The periderm, primary and secondary cortex, and xylems with medullary rays could be observed in the unstained sections. Primary and secondary cortex with fibers in the primary cortex became visible after staining with toluidine blue. Dragendorff’s reagent revealed the presence of alkaloids in the secondary cortex and secondary xylem, while in the pith no signs of alkaloid content was observed.
Fig. 2

Transverse section of the root of S. chamaedryfolia. Transverse sections of the secondary root of Spiraea chamaedryfolia, unstained (I), stained with 1% toluidine blue (II) and treated with Dragendorff reagent (III). (P periderm, C cortex, PC primary cortex, SC secondary cortex, X xylem, SX secondary xylem, MR medullary ray)


Plants may contain alkaloids in two forms: either as free base or as salts of organic acids. The compounds present in the free base form can be extracted with organic solvents, while those in the salt form can be extracted using diluted inorganic acids. Diterpene alkaloids, and especially esters, may be unstable, thus they require special handling. For this reason alcoholic extraction is considered to be the most cautious method. However, the diverse structure and the substitution pattern of diterpene alkaloid molecules might require acidic and alkaline extraction as well. According to the literature, only alcoholic extraction was applied in previous phytochemical screening studies of Spiraea species, which might have resulted in an incomplete extraction. To prevent the decomposition of the alkaloid content, the order of extraction was determined to be started by methanol, and followed by organic and acidic extraction steps. The application of all these three extraction methods yielded fractions with a diverse alkaloid profile.

Unfortunately, although 4.0 kg of dried roots was used for the preparative phytochemical work, our efforts to isolate pure alkaloids were unsuccessful. After purification with adsorption chromatography (i.e. column chromatography and centrifugal planar chromatography) and gel filtration chromatography, the polarity and the molecular size of alkaloids and matrix compounds were similar within the obtained fractions, rendering separation impossible. Beside the notable amount of matrix compounds the highly unstable manner of alkaloids was also an obstacle to isolate pure compounds.

Fractions of S. chamaedryfolia were found to exert noteworthy biological activities. Xanthine oxidase inhibitory activity of S. chamaedryfolia fractions was remarkable, and the fractions also exerted a moderate antibacterial activity.

Proving the presence of alkaloids in S. chamaedryfolia is noteworthy, since only few taxa are known to have the ability to produce diterpene alkaloids: it has previously been reported for S. japonica 64 [1029], S. fritchiana 2 [12, 16], S. koreana [30] and S. formosa 1 [31] only. No other types of alkaloids have been reported for the Spiraea genus. The alkaloid content of S. chamaedryfolia and the lack of alkaloids for S. crenata, S. media, S. salicifolia, S. nipponica, S. x vanhouttei and S. x billardii is first reported by our research group, making our phytochemical analyses pioneering in this field.


Only TLC detection methods were applied to confirm the alkaloid content, the subtypes of these alkaloids was not elucidated by LC–MS or NMR techniques. However, since no other alkaloid types have been reported from the Spiraea genus, this finding suggests the presence (or absence) of diterpene alkaloids in the investigated species.







ethyl acetate




liquid chromatography–mass spectroscopy




medullary ray


nuclear magnetic resonance




primary cortex


secondary cortex


secondary xylem


thin layer chromatography




xanthine oxidase


Authors’ contributions

TK and CD conceived and designed the experiments. SR, IP and AN provided and identified the plant material. CK and TK performed phytochemical experiments. Pharmacological investigations were performed by OO, EL, ZZ. TK, CK and CD analysed the data. Funding acquisition by CD. All authors contributed in drafting of the manuscript. All authors read and approved the final manuscript.


The authors thank Dora Bokor PharmD for proofreading the manuscript.

Competing interests

The authors declare that they have no competing interests.

Availability of data and materials

The dataset supporting the conclusions of this research is included in the article.

Consent for publication

Not applicable.

Ethics approval and consent to participate

Spiraea chamaedryfolia and Spiraea chamaedryfolia were collected in Daugavpills (Latvia). Spiraea crenata was collected in Alsórákos (Hungary) and Spiraea salicifolia in Sepsibükszád (Hungary). Spiraea nipponica, Spiraea x vanhouttei and Spiraea x billardii were collected in Botanical Garden, University of Szeged in Szeged (Hungary). None of the plant species used are endangered at their harvesting place, thus according to the country of origin, there was no need for permission or licence. Plant material was collected on public territory.


This work was supported by TÁMOP 4.2.4.A/2-11-1-2012-0001 ‘National Excellence Program’ (ÚNKP-ÚNKP-16-2 “New national excellence program of the Ministry of Human Capacities”); Hungarian Academy of Sciences (János Bolyai Research Scholarship); National Research, Development and Innovation Office (115796); GINOP-2.3.2-15-2016-00012 (New ways in the natural product-based drug discovery—system metabolomics approaches to discover biologically active terpenoids of herbal and microbial origin); TÁMOP 4.2.4.A/2-11-1-2012-0001 ‘National Excellence Program’ (ÚNKP-ÚNKP-16-2 “New national excellence program of the Ministry of Human Capacities”).

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Authors’ Affiliations

Department of Pharmacognosy, University of Szeged, Szeged, Hungary
Interdisciplinary Centre for Natural Products, University of Szeged, Szeged, Hungary
Department of Chemistry and Geography, Daugavpils University, Daugavpils, Latvia
Botanical Garden, University of Szeged, Szeged, Hungary


  1. Hegnauer R. The taxonomic significance of alkaloids. Chemistry plant taxonomy. Sand Diego: Academic Press; 1963. p. 389–427.View ArticleGoogle Scholar
  2. Culvenor C, Loder J, Nearn RH. Alkaloids of the leaves of Erythrophleum chlorostachys. Phytochemistry. 1971;10:2793–7.View ArticleGoogle Scholar
  3. Pelletier SW, Keith LH. Diterpene alkaloids from Aconitum, Delphinium, and Garrya species: the C19-diterpene alkaloids. In: Manske RHF, editor. Alkaloids Chem Physiol. London: Academic Press; 1970. p. 1–134.Google Scholar
  4. Dahlgren RMT. A revised system of classification of the angiosperms. Bot J Linn Soc. 1980;80:91–124.View ArticleGoogle Scholar
  5. Hao X, Shen Y, Li L, He H. The chemistry and biochemistry of Spiraea japonica complex. Curr Med Chem. 2003;10:2253–63.View ArticlePubMedGoogle Scholar
  6. Potter D, Still SM, Grebenc T, Ballian D, Božič G, Franjiæ J, et al. Phylogenetic relationships in tribe Spiraeae (Rosaceae) inferred from nucleotide sequence data. Plant Syst Evol. 2007;266:105–18.View ArticleGoogle Scholar
  7. Martini M, Lee I-M, Bottner KD, Zhao Y, Botti S, Bertaccini A, et al. Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas. Int J Syst Evol Microbiol. 2007;57:2037–51.View ArticlePubMedGoogle Scholar
  8. Shin H, Kim Y-D, Oh S-H. A new combination in Spiraea (Rosaceae) from Ulleung Island, Korea. Novon A J Bot Nomencl. 2011;21:373–4.View ArticleGoogle Scholar
  9. Khan G, Zhang FQ, Gao QB, Fu PC, Xing R, Wang JL, et al. Phylogenetic reconstruction between the old and new world spiroides inferred from plastid trnL-F and nrDNA its sequences. Pakistan J Bot. 2016;48:2399–407.Google Scholar
  10. Zuo GY, He HP, Hong X, Zhu WM, Hu YM, Yang XS, et al. New diterpenoid alkaloids from Spiraea japonica var. ovalifolia. Chin Chem Lett Chin Chem Soc. 2001;12:147–50.Google Scholar
  11. Zuo GY, He HP, Hong X, Zhu WM, Yang XS, Hao XJ. New spiramines from Spiraea japonica var. ovalifolia. Heterocycles Japan Inst Heterocyclic Chem. 2001;55:487–93.Google Scholar
  12. Li M, Du XB, Shen YM, Wang BG, Hao XJ. New diterpenoid alkaloids from Spiraea fritschiana var. parvifolia. Chin Chem Lett. 1999;10:827–30.Google Scholar
  13. Fan L-M, He H-P, Shen Y-M, Hao X-J. Two new diterpenoid alkaloids from Spiraea japonica L. f. var. fortunei (Planchon) Rehd. J Integr Plant Biol. 2005;47:120–3.View ArticleGoogle Scholar
  14. Gorbunov VD, Sheichenko VI, Ban’kovskii AI. New alkaloid from Spiraea japonica. Khimiya Prir Soedin. 1976;(1):124–5.Google Scholar
  15. Goto G, Sasaki K, Sakabe N, Hirata Y. The alkaloids obtained from Spiraea japonica L. Tetrahedron Lett. 1968;11:1369–73.View ArticlePubMedGoogle Scholar
  16. Wang F-P, Liang X-T. C20-diterpenoid alkaloids. IAlkaloids Chem Biol. 2002;59:1–280.View ArticleGoogle Scholar
  17. Yang X, Hao X. The diterpenoid alkaloids from Spiraea japonica var. glabra. Acta Bot Yunnanica. 1993;15:421–3.Google Scholar
  18. Wang B-G, Li L, Yang X-S, Chen Z-H, Hao X-J. Three new diterpene alkaloids from Spiraea japonica. Heterocycles. 2000;53:1343–50.View ArticleGoogle Scholar
  19. Shen YM, He HP, Zhang YS, Wang BG, Hao XJ. Spiramide, a new diterpene amide from the roots of Spiraea japonica var. acuta. Chin Chem Lett. 2000;11:789–92.Google Scholar
  20. Liu H-Y, Ni W, Chen C-X, Di Y-T, Hao X-J. Two new diterpenoid lactams from Spiraea japonica var. ovalifolia. Helv Chim Acta. 2009;92:1198–202.View ArticleGoogle Scholar
  21. Hao X, Node M, Taga T, Miwa Y, Zhou J, Chen S, et al. The structures of four new diterpene alkaloids, spiramines A, B, C, and D. Chem Pharm Bull (Tokyo). 1987;35:1670–2.View ArticleGoogle Scholar
  22. Nie JLJ, Hao XX. Spiramilactone B, a new diterpenoid from Spiraea japonica var. stellaris. Acta Bot Yunnanica. 1996;18:226–8.Google Scholar
  23. Node M, Hao X, Zhou J, Chen S, Taga T, Miwa Y, et al. Spiramines A, B, C, and D, new diterpene alkaloids from Spiraea japonica var. acuminata Franch. Heterocycles. 1990;30:635–43.View ArticleGoogle Scholar
  24. Hao X, Zhou J, Chen S, Fuji K, Node M. New diterpene alkaloids from Spiraea japonica var. acuminata. Acta Bot Yunnanica. 1991;13:452–4.Google Scholar
  25. Hao X, Node M, Zhou J, Chen S, Fuji K. Chemical structures of spiramine H, I and O. Acta Bot Yunnanica. 1994;16:301–4.Google Scholar
  26. Hao X, Zhou J, Fuji K, Node M. The chemical structures of spiramine J, K, L, and M. Acta Bot Yunnanica. 1992;14:314–8.Google Scholar
  27. Li L, Shen Y-M, Yang X-S, Wu W-L, Wang B, Chen Z-H, et al. Effects of spiramine T on antioxidant enzymatic activities and nitric oxide production in cerebral ischemia-reperfusion gerbils. Brain Res. 2002;944:205–9.View ArticlePubMedGoogle Scholar
  28. Wang B, Liu B, Zuo G, Hao X, Bingui W, Bin L, et al. New minor diterpenoid alkaloid from Spiraea japonica var. acuta. Yunnan Zhiwu Yanjiu. 2000;22:209–13.Google Scholar
  29. Fan L, Zhang Z, Shen Y, Hao X. Five diterpene alkaloids from Spiraea japonica (Rosaceae). Biochem Syst Ecol. 2004;32:75–8.View ArticleGoogle Scholar
  30. Jin KD. Studies on the constituents of Spiraea koreana Nakai. J Korean Chem Soc. 1967;11:111–6.Google Scholar
  31. Wu T-S, Hwang C-C, Kuo P-C, Kuo T-H, Damu AGAG, Su C-R. New neolignans from Spiraea formosana. Chem Pharm Bull (Tokyo). 2004;52:1227–30.View ArticleGoogle Scholar


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