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BMC Research Notes

Open Access

Draft genome sequence of Paenibacillus sp. EZ-K15 isolated from wastewater systems

BMC Research Notes201710:734

Received: 20 October 2017

Accepted: 5 December 2017

Published: 12 December 2017



Paenibacillus species, belonging to the family Paenibacillaceae, are able to survive for long periods under adverse environmental conditions. Several Paenibacillus species produce antimicrobial compounds and are capable of biodegradation of various contaminants; therefore, more investigations at the genomic level are necessary to improve our understanding of their ecology, genetics, as well as potential biotechnological applications.

Data description

In the present study, we describe the draft genome sequence of Paenibacillus sp. EZ-K15 that was isolated from nitrocellulose-contaminated wastewater samples. The genome comprises 7,258,662 bp, with a G+C content of 48.6%. This whole genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession PDHM00000000. Data demonstrated here can be used by other researchers working or studying in the field of whole genome analysis and application of Paenibacillus species in biotechnological processes.


Paenibacillus sp.Genome sequencingDraft genome assembly and annotationWastewater


Paenibacillus species, belonging to the family Paenibacillaceae, are rod-shaped Gram-positive or Gram-variable endospore forming aerobic or facultatively anaerobic bacteria, which are able to survive for long periods under adverse environmental conditions. Bacteria belonging to the genus Paenibacillus can be isolated from a wide range of environments including humans, animals, plants and the environment [1, 2]. Many species of Paenibacillus genus synthesize antimicrobial compounds that can be used as pesticides as well as in medicine, and many species produce enzymes important in bioremediation related technologies. Paenibacillus strains can be successfully applied for contaminants removal from a variety of wastewater systems. Also, several Paenibacillus strains can be involved in hydrolysis of cellulose and hemicellulose, lignin depolymerization and degradation of various textile dyes, polyvinyl alcohol, diesel fuel, bitumen, polycyclic aromatic hydrocarbons, benzene and other compounds [2]. Hence, more studies at the genomic level are important to clarify our understanding of their ecology, genetics, as well as potential biotechnological applications. Thus, Paenibacillus sp. EZ-K15 was isolated from nitrocellulose-contaminated wastewater systems, Kazan, Republic of Tatarstan, Russia [3]. These industrial wastes produce high levels of wastewaters polluted with multifarious dissolved chemical compounds and nitrocellulose particles. Therefore, isolation of bacteria which are able to transform various adverse pollutants and their genome analysis are of high importance for the creation of effective bioremediation strategies [4, 5].

Data description

Paenibacillus sp. EZ-K15 was isolated from nitrocellulose-contaminated wastewater environments, Kazan, Republic of Tatarstan, Russia [3]. The bacterium optimally grown on Luria agar at + 30 °C had been cultivated for 1–2 days. Genomic DNA of the bacterial strain EZ-K15 was extracted using a FastDNA spin kit (#116540600; MP Biomedicals) and a Super FastPrep-1 homogenizer (#116011500; MP Biomedicals) as detailed in the manufacturer’s protocol. Concentration and purity (A260/A280) of the obtained bacterial genomic DNA were measured using a NanoDrop 2000 spectrophotometer (#ND-2000; Thermo Fisher Scientific) and then stored at − 20 °C. The bacterial strain Paenibacillus sp. EZ-K15 was morphologically identified and then confirmed by PCR amplification applying universal primers UniBac27f, Bakt_805R and Univ1492r, followed by sequencing using an ABI PRISM 3130xl Genetic Analyzer (#4359571; Applied Biosystems) and phylogenetic analysis (16S rRNA gene sequence, 1437 bp, BLAST identity of 99% to Paenibacillus lautus; Table 1). In order to perform whole genome analysis, DNA was fragmented using a Q800R2 Sonicator (#Q800R2-110; Qsonica), and DNA library was then prepared using a NEBNext Ultra DNA Library Prep Kit for Illumina (#E7370S; New England Biolabs) according to the manufacturers’ protocols. Both efficiency of DNA fragmentation and DNA library preparation were monitored using a 2100 Bioanalyzer (#G2939BA; Agilent) and a High Sensitivity DNA kit (#5067-4626; Agilent). Further sequencing was performed using a high-throughput Illumina MiSeq platform (#SY-410-1003; Illumina) at Joint KFU-Riken Laboratory, Kazan Federal University (Kazan, Russia) by a MiSeq Reagent Kit v2PE 500 cycles (#MS-102-2003; Illumina). Sequence reads quality was assessed using PRINSEQ lite version 0.20.4 [6], the genome was assembled using Velvet version 1.2.10 [7], and the ordering of contigs was achieved using Mauve version 2.4.0 [8]. The whole genome sequence of Paenibacillus sp. EZ-K15 was annotated using the Rapid Annotation System Technology server [9]. The rRNA and tRNA genes were identified using RNAmmer 1.2 [10] and tRNA scan-SE 1.23 [11], respectively. Comparison of the genomic feature of Paenibacillus sp. EZ-K15 with several Paenibacillus strains was performed using data obtained from EzBioCloud database [12].
Table 1

Overview of data files/data sets


Name of data file/data set

File types (file extension)

Data repository and identifier (DOI or accession number)


Data file 1

Whole genome shotgun project


DDBJ/ENA/GenBank (accession PDHM00000000)


Data file 2

16S rRNA gene sequence


GenBank (accession MG050738)


The draft genome sequence of Paenibacillus sp. EZ-K15 composed of 36 contigs ranging from 512 to 911,265 bp with a total size of 7,258,662 bp, a G+C content of 48.6% and N50 of 242,001 bp. The Rapid Annotation System Technology server predicted 6682 coding sequences where 2551 coding sequences (39%) were annotated as seed subsystem features and 4131 coding sequences (61%) were annotated as outside of the seed subsystem. In total 4560 and 2122 coding sequences were assigned as non-hypothetical and hypothetical, accordingly. The genome was shown to encode at least 3 rRNAs and 66 tRNAs. The strain Paenibacillus sp. EZ-K15 possesses a substantial number of genes responsible for denitrification and nitrate/nitrite ammonification (e.g., for nitrate released during nitrocellulose denitration) as well as for metabolism of aromatic compounds, including genes involved in benzoate, gentisate and some other compounds biodegradation. Numerous genes responsible for resistance to toxic compounds, including arsenic, mercury, cadmium, as well as chromium compounds, were additionally detected. This resistant strain may have future usefulness in bioremediation of various sites.


Current data is based on the draft level genome sequence, due to which exact length of the genome, synteny, number of rRNA and repetitive elements cannot be reported. In addition, whether the genome consists of any plasmid/s or extra-chromosomal DNA cannot be certainly predicted.


Authors’ contributions

WSM and EEZ conducted experiments, performed genome analysis, interpretation of the data and drafted the manuscript. EIS and NEG carried out Illumina sequencing and revised the manuscript. AMZ supervised the project, designed the study, performed genome analysis and professionally revised the manuscript. All authors read and approved the final manuscript.


We thank Dr. Rushan Agzamov for support during the sampling.

Competing interests

The authors declare that they have no competing interests.

Availability of data materials

The data described in this Data note can be freely and openly accessed at DDBJ/ENA/GenBank. Please see Table 1 for details and links to the data.

Consent for publication

Not applicable.

Ethics approval and consent to participate

Not applicable.


The reported study was funded by the Russian Foundation for Basic Research [Grant No. 16-34-60093 mol_a_dk].

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Authors’ Affiliations

Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia
Department of Biotechnology, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
Laboratory of Extreme Biology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia


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