Materials and methods
hFasLECD-Avi conjugate was newly prepared according to the procedures in the previous paper [11]. The sample contained less than 10% of the unconjugated proteins as judged by the profile of size-exclusion chromatography (Additional file 1). FL-hFasLECD was prepared as described previously [10]. The conjugation ratio of fluorescein moiety at the reactive Cys residues was estimated to be 83%. Fluorescein conjugated bovine serum albumin (FL-BSA) was purchased from Thermo Fisher Scientific (A23015) and used as the negative control sample. Animal-derived-free recombinant human IFN-γ for cell biology was obtained from Wako Pure Chem. (093-06111) and used as a stock solution (1.0 mg/ml) dissolved in pure water. The biological activity and the endotoxin level were estimated to be 10 IU/ng and less than 0.01 ng/μg, respectively. All samples were kept frozen at below 253 K until use in the experiments.
The MALDI-TOF mass-spectrometric analysis was conducted by Brucker Ultra flex III-MALDI TOF/TOF mass-spectrometer in linear mode under the positive ionization condition using sinapinic acid as the matrix. Whole molecular-weight to electric charge ratio (m/z) measurement range was 10,000–300,000. Multiple peaks derived from BSA (average m/z: 5+, 13,287.00; 4+, 16,608.50; 3+, 22,144.30; 2+, 33,215.50; 1+, 66,430.00) were used for the calibration as the external standards. The hFasLECD-Avi conjugate at the concentration of 710 μg/ml in 25 mM Tris hydrochloride plus 15 mM sodium chloride (pH 7.5) was threefold diluted with pure water and subjected to analysis after mixed with the matrix solution at 1:1 ratio. Under the same condition, the measurement using matrix solution alone was performed to obtain control blank data for judging whether the peaks from the sample were true signals or not.
Human colorectal adenocarcinoma cell line, HT-29 cells (catalog number: EC91072201-GO, ECACC), used for the cell-viability measurement, were purchased from DS-Pharma Biomedical. The cells were maintained in McCoy’s 5A (Modified) medium (Thermo Fisher, 08457-55) supplemented with 10% FBS (Thermo Fisher, 10437-028) and 2% Penicillin–Streptomycin mixed solution (Nacalai Tesque, 09367-34) at 310 K in a 95% humidified air—5% CO2 incubator. Cell passages were carried out at 80% confluence at a ratio of 1:3. HT-29 cells were seeded at 3 × 103 cells/well in 150 μl of the medium in poly-l-lysine coated 96 wells microplates (Iwaki), and allowed to attach and grow for 24 h. Then, 50 μl of the medium containing IFN-γ (0, 0.1, 1.0, 10 or 100 IU/ml) was added and incubated for another 24 h. After that, the medium was changed to 200 μl of that containing either hFasLECD conjugate, FL-BSA or PBS alone sample, and further incubated for 4–72 h. Four hours earlier than the time of absorbance measurement, 10 μl of MTT solution (5 mg/ml in PBS) was added. The culture medium was removed, and 200 μl each of DMSO was added to dissolve the contents. Then, the absorbance at 535 nm was measured immediately. The serial dilution of the test samples was conducted using PBS. The cell-viability was evaluated as the value relative to the average of PBS alone sample. Four independent experimental data under each treatment condition were used for the calculation of average value and standard error of mean.
Results
Identification of structural components in hFasLECD-Avi conjugate
The MALDI-TOF mass-spectrometric analysis identified both signals derived from the subunits involved in and that irrelevant to the covalent linking. Figure 1 shows the spectra in m/z ranges of 10,000–30,000, 20,000–40,000 and 30,000–40,000. The signal derived from the covalently-linked subunits in hFasLECD-Avi conjugate was detected as the m/z peak at 36,854.0 (middle and lower panels). While, the signals, which can be attributed to the unconjugated hFasLECD-TCO and Avi-MTZ subunits, were observed as the m/z peaks at 10,319.3, 20,698.9 (upper and middle panels) and 15,895.5, 31,667.9 (all panels), respectively. The peak m/z value of the covalently-linked subunits was detected as 99.3% total of the corresponding values (20,698.9 and 15,895.5) for both unconjugated protein-derivative subunits. No appreciable m/z peak was present above 40,000, suggesting the absence of multiply-conjugated molecules (Additional file 2). These results showed a good agreement with the data of the SDS-PAGE and the size-exclusion chromatography analysis performed previously [11]. Consequently, it was confirmed that the conjugated sample possessed a covalent bond between the subunits belonging to hFasLECD and Avi and was suggested to be mostly composed of the 1:1 adducts of each protein.
Evaluation of cell-death inducing activity of FL-hFasLECD and hFasLECD-Avi conjugates
Cell-death inducing activity of FL-hFasLECD and the same purified hFasLECD-Avi conjugates were evaluated using MTT cell-viability assay. Figure 2 illustrates the concentration dependency of FL-hFasLECD on the relative viability of HT-29 cells in either the presence or absence of the pretreatment with human IFN-γ (100 IU/ml) (panel a). Also, the IFN-γ concentration dependency during the pretreatment prior to the addition of FL-hFasLECD or FL-BSA is shown (panels b and c). As shown in Fig. 2a, the relative viability of HT-29 cells was not essentially affected by the treatment with FL-hFasLECD under the pretreatment condition with no IFN-γ. The relative viability remained the same as in the cases of FL-BSA (1000 ng/ml) and PBS alone. In contrast, a prominent reduction in the cell-viability was observed for the treatment with FL-hFasLECD at concentrations of higher than 10 ng/ml after the IFN-γ pretreatment (100 IU/ml) for 24 h (panel a). It should be noted that also in the cases of FL-BSA and PBS alone, the pretreatment with 100 IU/ml of IFN-γ significantly reduced the cell-viability, which may reflect the small up-regulation of endogenous Fas ligand expression in HT-29 cells [13]. The IFN-γ concentration in the pretreatment affected the cell-viability (panels b and c). The cell-death induction against HT-29 cells was remarkably sensitized by the IFN-γ pretreatment concentration-dependently in the treatment with FL-hFasLECD at concentrations of both 100 and 1000 ng/ml. This stimulating effect was always substantial in the range from 0.1 to 100 IU/ml of IFN-γ in comparison with the cases of FL-BSA. Under the conditions using 100 IU/ml of IFN-γ and 100 or 1000 ng/ml of FL-hFasLECD, almost no viable cell was observed after 72 h treatment.
In Fig. 3, the experimental results of hFasLECD-Avi conjugate against HT-29 cells are summarized. The cell-viability after the treatment with either 100 or 1000 ng/ml of hFasLECD-Avi conjugate was again highly reduced by the pretreatment with 0.1–100 IU/ml of IFN-γ. The sensitizing effect was IFN-γ concentration dependent as was the case of FL-hFasLECD (panels a and b). Following 24 h pretreatment with 10 or 100 IU/ml of IFN-γ, almost no cells remained alive after 72 h treatment with either 100 or 1000 ng/ml of hFasLECD-Avi conjugate. For the purpose of investigating the initiation time of the cell-death, the cell-viability was checked after 4–72 h in the treatment with 100 ng/ml of hFasLECD-Avi conjugate, preceded by the pretreatment with either 0 or 100 IU/ml of IFN-γ (panels c and d). As expected, no significant difference in the cell-viability was observed between hFasLECD-Avi (100 ng/ml) and PBS alone samples in the case that IFN-γ was not included in the pretreatment. In contrast, a significant reduction in the cell-viability started from 8 h, and the level of cell-death was almost maximized after 12–24 h in the case that 100 IU/ml of IFN-γ was included. In both cases of FL-hFasLECD and hFasLECD-Avi, the microscopic observation revealed that the morphology of HT-29 cells changed from a transparent, expanded form to a dense, shrunk shape, which indicated the apoptotic cell-death (Additional file 3).
Discussion
In the MALDI-TOF mass-spectrometric analysis of hFasLECD-Avi conjugate, the intensity of the m/z peak derived from the component composed of covalently-linked hFasLECD-TCO and Avi-MTZ subunits appeared considerably weak relative to that from each single subunit derivative alone (Fig. 1), even after taking relative abundance of each component in the 1:1 conjugate into account. This suggested the difficulty in ionization of the linked component. The generally broadened peaks could be ascribed to the heterogeneity of the carbohydrate chains attached to the hFasLECD-TCO and Avi-MTZ subunits.
A remarkable cell-death of a colorectal cancer cell-line, HT-29 cells, was triggered by both site-specific conjugates of hFasLECD in synergy with the human IFN-γ pretreatment. This can be considered to be a mimic of the IFN-γ release by CTL and NK cells toward malignant cells in the human body. Recently, it was demonstrated that the treatment with IFN-γ augmented proliferation inhibition or apoptosis execution against various types of cancer cells by activating several signal transduction systems, including JAK-STAT 1 pathway [18, 19]. The enhancement of cell-death inducing activity in this study is also supposed to be linked with such signaling mechanisms.
Conclusions
In conclusion, the above experimental results confirmed the covalently-linked structure and the cell-death inducing activity in the site-specific chemical conjugates of hFasLECD. It is important to note that the death of HT-29 cells was strongly induced with the synergistic assistance of IFN-γ pretreatment under the conditions devoid of any cross-linking antibody. Our present study reinforces the way to the development of novel, clinically valuable molecular tools using hFasLECD as the conjugational component with the concrete structural and functional evidences. The combination with useful targeting molecules to diseased cells in the conjugation would contribute to the future development of the molecular tools aimed at medical applications.
Limitations
The major limitations of this study are that only two site-specific chemical conjugates of hFasLECD and a single cancer cell-line were used as the test samples for the examinations and the cells employed in the cell-viability assay, respectively. There remains a necessity for the evaluation using various types of conjugate samples and target cell-lines in order to generalize the conclusions.
