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Novel unconditioned prosocial behavior in prairie voles (Microtus ochrogaster) as a model for empathy
BMC Research Notes volume 11, Article number: 852 (2018)
In this study, empathy is quantified using a novel social test. Empathy and prosocial behavior are linked to the expression of oxytocin in humans and rodent models. Specifically, prosocial behavior in prairie voles (Microtus ochrogaster) has been linked to the expression of oxytocin in the paraventricular nucleus of the hypothalamus. The animal’s behavior was considered empathic if it spends significantly more time attempting to remove a loos fitting restraint (tether) from the stimulus animal than time in contact with a, simultaneously presented, non-social object similar to the tether. The behavioral data was cross-referenced with the number of neurons expressing oxytocin and arginine vasopressin, as well as the density of dopaminergic neurons (identified by the expression of tyrosine hydroxylase), in the paraventricular nucleus of the hypothalamus. These proteins influence empathic behavior in humans, non-human primates, rats, mice, and prairie voles.
The consistency between neuroanatomical mechanisms linked to empathy, and the durations of time spent engaging in empathic contact, support the prediction that the empathic contact in this test is a distinct prosocial behavior, lacking prior behavioral training or the naturally occurring ethological relevance of other prosocial behaviors, and is a measure of empathy.
In humans, observing distress drives signaling between the cingulate cortex, prefrontal cortex, and limbic regions, such as the amygdala and hypothalamus . This correlates with self-reported empathy, an external expression of which is prosocial behavior . Prosocial behaviors have been linked to the expression of oxytocin (OT) in humans [3, 4]. In prairie voles (Microtus ochrogaster), OT regulates prosocial behaviors, such as partner preference formation, alloparental care, and consoling behavior [5,6,7,8]. Specifically, prosocial behavior in prairie voles has been linked to the expression of OT in the paraventricular nucleus of the hypothalamus (PVN) [9, 10]. The PVN signals to the pituitary and the supraoptic nucleus, triggering a cascade of signaling molecules that modulate the regulatory mechanisms of steroid-dependent prosocial behaviors such as pair-bonding and alloparental care, through estrogen receptor alpha (ERα) . It has been hypothesized that OT’s role as an empathogen may have first evolved in the context of kinship relationships, and that the ability to accurately infer others emotions may provide an advantage through the ‘trust-effect’ of OT . The mechanism by which OT influences empathy is currently unknown, but if it is an extension of the mechanism by which it regulates other steroid-dependent prosocial behaviors, it is likely to modulate corticolimbic signaling patterns  through ERα.
In this study, neurons expressing OT, arginine vasopressin (AVP), or tyrosine-hydroxylase (TH) (dopaminergic neurons), were counted in the PVN of prairie voles. While OT, AVP and dopamine in the PVN are linked to social behaviors like exploratory sniffing [14,15,16], only OT and AVP in the PVN are linked to empathy [7, 12, 17,18,19,20]. A role for dopaminergic PVN neurons, in the context of empathy, remains unexplored. Dopamine in the anterior cingulate cortex  and nucleus accumbens, however, is important in empathy-like responses to observational fear. In prairie voles, males have more dopaminergic neurons in the PVN than females , but not OT neurons . Here we sought to determine if there is an unconditioned prosocial behavior, distinct from other prosocial behaviors, that is indicative of empathy and linked to PVN OT neurons.
Quantifying empathic behavior in rodents often requires specific training reinforced by reward [2, 7, 23]. In these studies, a test animal observes a stimulus animal expressing distress from foot shock [20, 23] or forced restraint , and the test animal can perform a specific task to eliminate the distress, such as pulling a lever. The training and reward confounds empathic behavior as, potentially, a product of training. Other rodent models of empathy utilize a freezing response in an observer animal that witnesses distress [7, 8, 20, 24]. However, freezing behavior can result from social transmission of fear [25, 26] or empathy. Allogrooming between a distressed animal and cage mate, or conspecific is the strongest prosocial measure of empathy [7, 27], but grooming can be aggressive and dominance driven . The functions of grooming complicate its interpretation in rodent models of dominance driven social hierarchy, such as rats . The advantage of the behavior used in the present study is that it does not require training or social fear, and lacks known alternative ethological functions. Here, we use a novel social test similar to those used in social preference tests [29, 30] (Fig. 1). The test animal is given no specific training or reward, and the behavior considered empathic is time spent attempting to remove a loose fitting restraint tether from an unrelated, same sex, size and age matched stimulus animal, vetted as non-aggressive.
To establish this behavior as empathic, time spent engaging in exploratory sniffing and time spent in contact with a non-social object were quantified. A significant difference in time in empathic contact and time in contact with the non-social object would suggest that the empathic contact is prosocial. The prosocial behavior most similar to empathic contact is exploratory sniffing, and a significant difference in empathic contact and exploratory sniffing would suggest that these are separate prosocial behaviors. If, as predicted, the empathic contact is a distinct prosocial behavior, without training or the ethological relevance of other prosocial behaviors, we conclude that it is a measure of empathy.
Study animals originated from an Urbana, IL wild stock and were used in a prior experiment . The prairie vole is a rodent model for investigating prosocial behavior . Prairie voles differ from traditional laboratory rodents in specific housing, diet and husbandry requirements [30,31,32].
Novel social test
Behavioral data recordings were taken for another, previously published experiment with different endpoints and goals . Only the control animals of both sexes from that prior study were used for the work herein. Briefly, on post-natal day 30, the animals were tested for 1 h in a novel social arena as described previously [5, 33] and in Fig. 1, using a gently tethered, unrelated stimulus animal (same sex, size, and age matched). The tether was a restraint loosely fit around the stimulus animal’s neck on one end and fixed to the wall mounted non-social object (represented in Fig. 1 as an elongated grey oval with a red outline) preventing the test animal from entering the non-social chamber or the adjoining tube. Attached to the cage wall was a non-social object of the same material and luster as the tether that was not directly linked to the collar portion of the animal’s tether.
A treatment-blinded observer scored behaviors using Jwatcher v1.0 behavioral scoring software (UCLA) . The measured behaviors included total test time spent in exploratory sniffing, chewing or pulling on tether/collar within 1.3 cm of the stimulus animal (empathic contact), and time spent engaging in contact with non-social object. An observer remained in the room for the duration of the test to monitor potential signs of aggression; no injury to any animals occurred.
Brain collection and preparation for immunohistochemistry
Brains were collected and processed as described in our prior publication . Again, only the control animals were used for the experiments described herein. Briefly, the animals were euthanized between post-natal days 60–90. Each animal was given 0.05-mL buprenorphine ip and then deeply anesthetized 15 min later with 0.05 mL of ketamine–xylazine (at a concentration of 67.7 and 13.33 mg/kg, respectively) mixture administered sc. Brains were collected and immersion fixed for 24 h in 4% paraformaldehyde and transferred to fresh solution at 2 and 4 h. 30% buffered sucrose with 0.1% sodium azide was used to cryoprotect the brains, which were then shipped to the Patisaul Lab, where they were stored in fresh cryoprotectant overnight at 4 °C and then flash frozen and stored at − 80 °C. Brains were sectioned at 35 µm coronally on a frozen sliding microtome. Subsequently, sections for each individual brain corresponding to the regions of interest were collected and processed for immunohistochemistry.
For each test animal, 8 sequential sections of the PVN were collected and processed for immunohistochemical staining of AVP and OT using routine procedures described previously  using 1:12,000 monoclonal mouse anti-OT (catalog number MAB5296; Millipore) and 1:12,000 polyclonal rabbit anti-AVP (catalog number 20069; Immunostar). Sections were then washed and incubated for 120 min in a solution of Alexa Fluor 568 goat anti-mouse and Alexa Fluor 488 goat anti-rabbit secondary antibodies (both at 1:200). Immunohistochemical staining of TH was performed on two consecutive posterior PVN sections as described previously  with the polyclonal rabbit anti-TH (catalog number AB152; Millipore) primary antibody at 1:4000 and Alexa Fluor 488 donkey anti-rabbit secondary antibody at 1:200. These sections were also counterstained via a 45-s incubation with Hoechst (catalog number H3569; Invitrogen Life Technologies). After a final wash in cold KPBS, all PVN sections were mounted on Fisher super frost plus glass slides, coverslipped with a glycerol mountant, and stored at − 20 °C.
Quantification and analysis
The 8 sequential sections selected for OT immunoreactivity (OT-ir) and AVP immunoreactivity (AVP-ir) quantification encompassed the entire PVN, as previously described . For the present study, the PVN subregional counts, established previously , were averaged within animal and then animal specific means were averaged by sex, so that each animal contributed one value to the final mean. The dopaminergic immunoreactive cell counts (TH-ir) are the same as previously reported .
All analyses were done with IBM SPSS Statistic version 21. Novel social results were analyzed by a one-way ANOVA testing for significant (P ≤ 0.05) sex-specific differences (homogeneity of variance was validated using Levene’s test, there were no distribution effects of unequal sample sizes). Paired t tests (2-tailed) were performed within sex testing for significant (P ≤ 0.05) differences in total test time between empathic behavior and non-social object contact, or empathic behavior and exploratory sniffing (males n = 9, females n = 22).
Significant (P ≤ 0.05) sex-specific differences in numbers of OT-ir, AVP-ir and TH-ir neurons in the PVN were determined by a one-way ANOVA. Significant (P ≤ 0.05) within-sex differences in the number of neurons expressing OT, AVP and TH were determined by a one-way ANOVA (OT and AVP males and females n = 11, TH males and females n = 7).
The behavioral results indicate that, both males (8 of 9) and females (20 of 22) engage in empathic contact by attempting to remove the tether from the stimulus animal (Fig. 2b). In the paired test results, males and females spent significantly more time engaging in contact with an object of potential empathic consequence versus a similar non-social object of no social consequence (paired t-test, male t8 = 3.77 P ≤ 0.005; female t21 = 3.09 P ≤ 0.05). However, only males spent significantly more time engaging in exploratory sniffing versus empathic contact (paired t-test, male t8 = − 2.29 P ≤ 0.05). Overall, males spent significantly more time engaging in exploratory sniffing than females (ANOVA F(1,20) = 6.18, P ≤ 0.05) (Fig. 2a).
As expected, the immunohistochemistry results revealed that the number of OT-ir and AVP-ir neurons across the PVN are not sexually dimorphic, but that the density of dopaminergic neurons in the posterior PVN is sexually dimorphic (Fig. 2c). Significantly more TH-ir neurons were observed in males than females (ANOVA F(1,12) = 8.76 P ≤ 0.05). Males also had greater numbers of OT-ir neurons than AVP-ir neurons (paired t-test, maleOT-AVP t10 = 30 P ≤ 0.001). Additionally, there were more TH-ir neurons than AVP-ir neurons in males, but there was no significant difference between the number of OT-ir and TH-ir neurons. In the PVN of females, there were significantly fewer AVP-ir neurons and TH-ir neurons than OT-ir neurons (paired t-test, femaleOT-AVP t11 = 12.23 P ≤ 0.001; femaleOT-TH t6 = 4.17 P ≤ 0.05), with no significant difference between AVP-ir and TH-ir neurons. Taken together, the results of this study suggest sex-specific differences in the expression of prosocial behavior, with concordant patterns of sexually dimorphic significance in the underlying neurobiology of the PVN. It is important to note that while region and neuron specific histology provides a broad understand of the underlying mechanisms contributing to prosocial behavior, it is the OT and AVP signaling that guides behavior.
Here we used behaviorally observable exploratory sniffing to measure sexually dimorphic non-empathic prosocial behavior. Males spent significantly more time engaging in empathic contact than non-social object contact, suggesting that empathic contact is prosocial (Fig. 2a). Additionally, the significant difference between exploratory sniffing and empathic contact suggests that empathic contact is distinct from non-empathic prosocial behaviors (Fig. 2a).
Because empathy is regulated by OT and AVP, but not dopaminergic PVN neurons, in humans and voles [7, 12, 17,18,19] we hypothesized that if empathic contact is distinct from other prosocial behaviors, then the number of OT-ir, AVP-ir and TH-ir neurons in the PVN would support this difference. Our measure of non-empathic prosocial behavior, exploratory sniffing, was similar across sexes as were the number of TH-ir neurons in the PVN. However, as predicted, the number of TH-ir PVN neurons across sexes was not did not parallel the duration of empathic contact between males and females. This observation is consistent with prior work showing that dopamine expression in the cingulate cortex is linked to empathy, but expression in the PVN is not . Our results support region-specific differences and suggest that dopaminergic neurons in the PVN may regulate exploratory sniffing and possibly other non-empathic prosocial behaviors.
Also consistent with our hypothesis, time spent in empathic contact and the number of AVP-ir and OT-ir neurons in the PVN, followed similar patterns across sexes (Fig. 2c). These data support prior work showing that empathy is regulated by oxytocinergic and vasopressinergic systems in the PVN of humans, rats and prairie voles [7, 8, 18, 19]. The number of PVN neurons expressing AVP and OT is independent of sex and consistent with the time spent in empathic contact versus contact with a non-social object across sexes. Finally, the neuroanatomical mechanisms that regulate empathy in prairie voles , and the time spent in empathic contact, support the prediction that empathic contact is a distinct prosocial behavior, independent of training or known ethological relevance, and is a measure of empathy. All supporting data can be accessed through the Additional file 1.
The cell counts used in this study provide strong correlative evidence for the role of PVN OT, AVP, and TH neurons in empathic behavior, but to demonstrate causal influence of these neuronal populations would require in vivo manipulations of the discussed cell populations. Reduced empathic contact concurrent with optogenetic silencing of PVN OT or AVP neurons using a noninvasive red-shifted microbial rhodopsin  would provide strong evidence for a causal role of these cells in empathy. To a lesser extent the use of pharmacological manipulations, such as OT or AVP antagonists could provide similar support, but could not account for off target effects from the peripheral nervous system, or other brain regions that express OT such as the olfactory tubercle .
paraventricular nucleus of the hypothalamus
Völlm BA, Taylor ANW, Richardson P, Corcoran R, Stirling J, McKie S, et al. Neuronal correlates of theory of mind and empathy: a functional magnetic resonance imaging study in a nonverbal task. Neuroimage. 2006;29:90–8.
Bartal B-A, Decety J, Mason P. Empathy and pro-social behavior in rats. Science. 2011;334:1427–31.
Hurlemann R, Patin A, Onur O, Cohen MX, Baumgartner T, Metzler S, et al. Oxytocin enhances amygdala-dependent, socially reinforced learning and emotional empathy in humans. J Neurosci. 2010;30:4999–5007.
Abu-Akel A, Palgi S, Klein E, Decety J, Shamay-Tsoory S. Oxytocin increases empathy to pain when adopting the other- but not the self-perspective. Soc Neurosci. 2014;10:1–9.
Cushing BS, Carter CS. Peripheral pulses of oxytocin increase partner preferences in female, but not male, prairie voles. Horm Behav. 2000;37:49–56.
Perkeybile AM, Delaney-Busch N, Hartman S, Grimm KJ, Bales KL. Intergenerational transmission of alloparental behavior and oxytocin and vasopressin receptor distribution in the prairie vole. Front Behav Neurosci. 2015;9:191. https://doi.org/10.3389/fnbeh.2015.00191/abstract.
Burkett JP, Andari E, Johnson ZV, De Curry DC, Waal FBM, Young LJ. Oxytocin-dependent consolation behavior in rodents. Science. 2016;351:375–8.
Meyza KZ, Bartal IB, Monfils MH, Panksepp JB, Knapska E. The roots of empathy: through the lens of rodent models. Neurosci Biobehav Rev. 2017;76:216–34.
Sullivan AW, Beach EC, Stetzik LA, Perry A, D’Addezio AS, Cushing BS, et al. A novel model for neuroendocrine toxicology: neurobehavioral effects of BPA exposure in a prosocial species, the prairie vole (Microtus ochrogaster). Endocrinology. 2014;155:3867–81.
Yamamoto Y, Cushing BS, Kramer KM, Epperson PD, Hoffman GE, Carter CS. Neonatal manipulations of oxytocin alter expression of oxytocin and vasopressin immunoreactive cells in the paraventricular nucleus of the hypothalamus in a gender-specific manner. Neuroscience. 2004;125:947–55.
Cushing BS. The organizational effects of oxytocin and vasopressin: behavioral implications. In: Oxytocin, Vasopressin Relat Pept Regul Behav. E. Choleris M. Kavaliers DWP, editors. Cambridge: Cambridge University Press; 2013. p. 56–66.
Bethlehem RAI, van Honk J, Auyeung B, Baron-Cohen S. Oxytocin, brain physiology, and functional connectivity: a review of intranasal oxytocin fMRI studies. Psychoneuroendocrinology. 2013;38:962–74.
Petrovic P, Kalisch R, Singer T, Dolan RJ. Oxytocin attenuates affective evaluations of conditioned faces and amygdala activity. J Neurosci. 2008;28:6607–15.
Dharmadhikari A, Lee YS, Roberts RL, Carter CS. Exploratory behavior correlates with social organization and is responsive to peptide injections in prairie voles. Ann N Y Acad Sci. 1997;807:610–2.
Bales KL, van Westerhuyzen JA, Lewis-Reese AD, Grotte ND, Lanter JA, Carter CS. Oxytocin has dose-dependent developmental effects on pair-bonding and alloparental care in female prairie voles. Horm Behav. 2007;52:274–9.
Cavanaugh BL, Lonstein JS. Androgenic and oestrogenic influences on tyrosine hydroxylase-immunoreactive cells of the prairie vole medial amygdala and bed nucleus of the stria terminalis. J Neuroendocrinol. 2010;22:217–25.
Benarroch EE. Oxytocin and vasopressin Social neuropeptides with complex neuromodulatory functions. Neurology. 2013;80:1521–8.
Uzefovsky F, Shalev I, Israel S, Edelman S, Raz Y, Mankuta D, et al. Oxytocin receptor and vasopressin receptor 1a genes are respectively associated with emotional and cognitive empathy. Horm Behav. 2015;67:60–5.
Ebstein RP, Israel S, Lerer E, Uzefovsky F, Shalev I, Gritsenko I, et al. Arginine vasopressin and oxytocin modulate human social behavior. Ann N Y Acad Sci. 2009;1167:87–102.
Pisansky MT, Hanson LR, Gottesman II, Gewirtz JC. Oxytocin enhances observational fear in mice. Nat Commun. 2017;8:2102.
Kim BS, Lee J, Bang M, Seo BA, Khalid A, Jung MW, et al. Differential regulation of observational fear and neural oscillations by serotonin and dopamine in the mouse anterior cingulate cortex. Psychopharmacology. 2014;231:4371–81.
Young KA, Gobrogge KL, Liu Y. Wang Z. Front. Neuroendocrinol: The neurobiology of pair bonding: insights from a socially monogamous rodent; 2011. p. 53–69.
Kelly JB, Masterton B. Auditory sensitivity of the albino rat. J Comp Physiol Psychol. 1977;91:930–6.
Lü Y-F, Yang Y, Li C-L, Wang Y, Li Z, Chen J. The locus coeruleus-norepinephrine system mediates empathy for pain through selective up-regulation of P2X3 receptor in dorsal root ganglia in rats. Front Neural Circuits. 2017. https://doi.org/10.3389/fncir.2017.00066/full.
Kim EJ, Kim ES, Covey E, Kim JJ. Social transmission of fear in rats: the role of 22-kHz ultrasonic distress vocalization. PLoS ONE. 2010;5:e15077.
Jones CE, Monfils MH. Dominance status predicts social fear transmission in laboratory rats. Anim Cogn. 2016;19:1051–69.
Lu YF, Ren B, Ling BF, Zhang J, Xu C, Li Z. Social interaction with a cagemate in pain increases allogrooming and induces pain hypersensitivity in the observer rats. Neurosci Lett. 2018;662:385–8.
Fontenot J, Loetz EC, Ishiki M, Bland ST. Monoacylglycerol lipase inhibition alters social behavior in male and female rats after post-weaning social isolation. Behav Brain Res. 2017;341:146–53.
Liu Y, Aragona BJ, Young KA, Dietz DM, Kabbaj M, Mazei-Robison M, et al. Nucleus accumbens dopamine mediates amphetamine-induced impairment of social bonding in a monogamous rodent species. Proc Natl Acad Sci USA. 2009;107:1217–22.
Cushing BS, Martin JO, Young LJ, Carter CS. The effects of peptides on partner preference formation are predicted by habitat in prairie voles. Horm Behav. 2001;39:48–58.
Carter CS, Witt DM, Thompson EG, Carlstead K. Effects of hormonal, sexual, and social history on mating and pair bonding in prairie voles. Physiol Behav. 1988;44:691–7.
Carter CS, Getz LL, Gavish L, McDermott JL, Arnold P. Male-related pheromones and the activation of female reproduction in the prairie vole (Microtus ochrogaster). Biol Reprod. 1980;23:1038–45.
Insel TR, Preston S, Winslow JT. Mating in the monogamous male: behavioral consequences. Physiol Behav. 1995;57:615–27.
Stetzik L, Ganshevsky D, Lende MN, Roache LE, Musatov S, Cushing BS. Inhibiting ERα expression in the medial amygdala increases prosocial behavior in male meadow voles (Microtus pennsylvanicus). Behav Brain Res. 2018;351:42–8.
Chuong AS, Miri ML, Busskamp V, Matthews GAC, Acker LC, Sørensen AT, et al. Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Nat Neurosci. 2014;17:1123–9.
Oettl LL, Ravi N, Schneider M, Scheller MF, Schneider P, Mitre M, et al. Oxytocin Enhances Social Recognition by Modulating Cortical Control of Early Olfactory Processing. Neuron. 2016;90:602–21.
LAS: Primary author, empathy assay design and quantification, tissue collection, statistical analysis of behavioral and neuroanatomical data. AWS: Collection of autoradiography data and quantification of neuroanatomy, manuscript revision. HBP: Study design and support, autoradiography/histology training, and manuscript revision. BSC: Study design and support, behavioral testing training, histology training, and manuscript revision. All authors read and approved the final manuscript.
The authors wish to thank Prof. Troy Ghashghaei from NCSU Department of Molecular Biomedical Sciences for providing access to his confocal fluorescence microscope used to image the tissue sections in this study.
Authors declare that there is no conflict of interest, financial interest or otherwise, that could have influenced the objectivity of the observations, results or interpretation of the findings.
Availability of data and materials
All data used in this study is available in the additional supporting documents.
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Ethic approval and consent to participate
Human subjects were not involved in this study, all related declaration statements are non-applicable. All animals were housed in accordance with USDA and NIH guidelines and all procedures were approved by the University of Akron and the Northeast Ohio Medical University Animal Care and Use Committee prior to undertaking any experimental manipulation.
The sources of funding for this study came from institutional funds available at North Carolina State University as well as a grant from the National Institute of Environmental Health Sciences, Award Number: ES021233, awarded to Dr. Heather B. Patisaul.
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Stetzik, L.A., Sullivan, A.W., Patisaul, H.B. et al. Novel unconditioned prosocial behavior in prairie voles (Microtus ochrogaster) as a model for empathy. BMC Res Notes 11, 852 (2018). https://doi.org/10.1186/s13104-018-3934-0
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