- Research note
- Open Access
Monomorium ant is a carrier for pathogenic and potentially pathogenic bacteria
© The Author(s) 2019
- Received: 7 March 2019
- Accepted: 11 April 2019
- Published: 16 April 2019
Household ants are regarded as a major household pest and their close association with microorganisms and people means that they may constitute a disease risk. Our study is the first to provide information on the pathogenicity of Monomorium spp. a common insect in Kuwait by quantifying and identifying the exoskeleton bacterial burden. Samples of Monomorium were collected in June from indoor and outdoor sites of 30 houses located in two residential districts.
The study identified a total of 16 different species of Gram-negative bacteria of which the indoor isolates were 75% greater in species count than the outdoor samples. Indoor isolates identified from both districts were more frequent than the outdoors and similar trends were obtained for a single district. Outdoor ant samples on the other hand carried a high percentage of bacteria but with less diversity in both districts. There was a significant variability in bacterial species in relation to sample sources, indoor and outdoor, and discrete geographical location. The presence of a high percentage of pathogenic and potentially pathogenic bacteria indoor poses a great threat to domestic households, which would be further exacerbated in places with poor standards of hygiene.
- Ant pathogenicity
- Bacterial types
- Risk on health
- House hygiene
- Infection by Salmonella
- Pathogen carrier
Many insects are important disease vectors facilitating the transmission of various microbes such as viruses, bacteria, fungi and nematodes . Among those insects are some social ones such as ants, which can transmit diseases and cause allergic reactions [2, 3]. As social insects, ants generally occur in large colonies and often enter buildings where inadequate control practices and improper storage of food that might aggravate their infestation [4, 5].
Monomorium ants pose major public-health challenges due to their potential to spread infectious diseases and their resistance to multiple types of infection . A number of ant species have become pests in many countries worldwide, invading households and residential units and posing significant health hazards. For instance, the sting of the black ant, Pachycondyla sennaarensis, causes allergic reactions ranging from mild to anaphylactic . In the United States, the fire ant Solenopsis spp. and carpenter ant Camponotus spp. are considered serious pests in almost 6% of households, and Monomorium pharaonis L. and Monomorium minimum and other species pose threats to 13% of residential units [8–10] and may cause food poisoning that represent a danger to patients in hospital environments . Nevertheless, bacterial diseases transmitted via food present a public health problem all over the world leads to high mortality and morbidity rates, .
Ants and bacterial associations have been detected in many hospitals, raising concerns about the possible role of ants as a disease vectors and microbial dispersal [13–15]. Several studies conducted in hospitals have shown a mutualistic relationship between ants and the presence of bacteria found on the ants’ exoskeleton . A study performed in Brazilian hospitals showed that the ants Paratrechina spp. and Monomorium floricola have high capacity of reproduction and colonization rates . Bacterial isolates from those ants revealed 68.8% of Bacillus spp. and Listeria spp., 14.7% of Pseudomonas aeruginosa and Klebsiella spp., and 16.4% of Streptococcus spp. and Staphylococcus aureus. These bacteria are responsible for hospital-acquired (nosocomial) infections and associated with potentially serious medical problems [16, 17]. Microbiological analysis of the cuticle of the genus Pheidole (subfamily Myrmicinae) revealed (90%) of Staphyloccocus spp. with low frequency of Klebsiella spp. (2%), Enterococcus spp. (2%) and Vibrio cholera (2%) .
Monomorium spp. is a common insect frequently found indoors and outdoors in residential and non-residential buildings in Kuwait. The emergence of antimicrobial resistance among the most important pathogenic bacteria coupled with the increase in ant colonies could lead to radical health consequences, ineffective infestation control measures  and eventually, high economic and social costs . It is thus necessary to evaluate its potential pathogenicity for raising awareness of its dangers. Therefore, the aim of this study was to determine and compare the concentration of air borne bacteria conveyed by the ant Monomorium from indoor and outdoor areas of two different districts. To the best of our knowledge no such study has been attempted on Monomorium in Kuwait.
Materials and methods
Using gloves, worker ants of Monomorium were collected from outdoor (backyard) and indoor (living room) sites of 30 houses in Al-Rowda (44.1 °C and RH 52%) and Bayan districts (41.3 °C and RH 74%) during the day. In the laboratory, the ants were randomly picked from containers using forceps and transferred into sterile dilution bottles containing peptone water. Ten milliliters of sterile normal saline (0.9%) was added to each test tube containing 30 ants and the tubes were thoroughly shaken for 2 min to isolate microorganisms from the external surface. Serially diluted (10−1 to 10−7) 0.1 ml aliquots were then separately inoculated onto nutrient agar plates and incubated overnight at 37 °C. Bacterial colonies were initially identified by morphological appearance, microscopic examination using staining techniques, and identified further by biochemical tests and were characterized on an API 20E (BioMerieux, France). The overall load of bacteria carried by each ant was quantified and expressed in colony- forming units (CFUs). Gram’s staining was carried out to find the reactions of the bacterial isolates following Muhammad et al. .
The means of numbers of CFU/0.1 ml aliquots obtained in three different replicates using 30 ants
Number of CFU/0.1 ml aliquots (mean ± SE)
(API 20 E)
7.0 ± 0.2 × 105
5.0 ± 0.1 × 105
4.0 ± 0.2 × 105
3.0 ± 0.1 × 105
2.0 ± 0.0 × 105
2.0 ± 0.0 × 105
1.0 ± 0.0 × 105
1.0 ± 0.0 × 105
8.0 ± 0.2 × 105
6.0 ± 0.2 × 105
4.0 ± 0.1 × 105
2.0 ± 0.1 × 105
1.0 ± 0.0 × 105
1.0 ± 0.0 × 105
8.0 ± 0.2 × 105
6.0 ± 0.2 × 105
4.0 ± 0.2 × 105
4.0 ± 0.2 × 105
6.0 ± 0.2 × 105
4.0 ± 0.2 × 105
2.0 ± 0.1 × 105
1.0 ± 0.2 × 105
The quantitative comparisons between bacterial populations showed a significant quantity and great diversity of bacteria carried by indoor ants compared with outdoor ones. Ant infestations are therefore likely to increase the rate of bacterial dissemination inside human premises and cause infectious and non-infectious adverse health in residential spaces and workplaces at any time . Several studies have mentioned the possible sources for indoor bacteria that often come from human shed skins and natural residues since people spend more than 90% of their time in these indoor environments [21, 22].
The isolation of E. coli species in this study from indoor ant samples is of great concern. This bacterium is one of the most commonly examined Gram-negative bacteria in microbiology. Gram-negative bacteria are known to cause sick house building syndrome and cause illness due to the production of endotoxins . Nordell et al.  reported that high exposure to endotoxins is often associated with nausea and diarrhea. Though it is well known that it inhabits the human bowel as part of normal microbiota, some strains can cause a chronic diarrhea and sever intestinal infections . Additionally, E. coli is the most common etiologic agent of urinary tract infections that may results in cystitis or ends in life threatening sepsis . Another important genus identified by this study were the Salmonella which are facultative intracellular pathogens and are recognized as the major causative agents of gastroenteritis and salmonellosis .
The analysis of bacterial colony counts of indoor ant samples from Bayan revealed one antibiotic-resistant bacterium Serratia marcescens that causes a wide range of infection such as, urinary and respiratory infections, endocarditis, osteomyelitis, septicemia, wound infections, eye infections, and meningitis which formed 30% of the sample. Some of the other species identified, such as, Shigella, Proteus, Klebsiella, Enterobacter, Citrobacter, Salmonella and Yersinia are also of medical significance as they can cause severe diarrhea and abdominal distress . Klebsiella pneumoniae, and Proteus mirabilis were also isolated from the ants’ body. They are normal commensal microflora but they may cause opportunistic infection such as hemorrhagic colitis, urinary infection and pneumonia [29–31].
There are several reasons for the augmented high percentage frequency of occurrence in indoor samples, the most important one is the minimal use of disinfection procedures against this insect because people are unaware of the health hazards associated with ants. In addition, dust accumulation coupled with high humidity may lead to the proliferation of microorganisms capable of surviving the prevailing conditions . Indoor air bacteria often originate from outdoors carried in the air in addition to anthropogenic sources such as residents’ activities [21, 32]. Poor aeriation elevates the survival rate of airborne organisms . Eventually, populations of airborne bacteria become high enough to pose a major issue in indoor environments, in particular domestic places and communal areas .
The burden of infectious diseases has worsened with the emergence of antimicrobial bacterial resistance, in particular those associated with ants . The current finding highlights the pathogenicity of Monomorium spp. ant as it carries, relative to its small body, a wide diversity of pathogenic bacterial species via mechanical means. For instance, the most common organism obtained indoors and outdoors was Acinetobacter lwoffii. This bacterium causes serious and sometimes life-threatening infections such as gastrointestinal and urinary tract infections, pneumonia and meningitis . In addition, it is able to survive dry conditions, low pH, and a wide range of temperature levels , and is also recognized as a common causative agent of various human diseases such as catheter-associated infections in immunocompromised patients [35, 37].
The comparative analysis showed variability in the integument bacterial composition among the analyzed districts. The studied districts, Bayan and Al-Rowda, differed with respect to location and each is characterized by specific meteorological parameters such as relative humidity. Bayan district is in close proximity to the sea side and is often characterized by high relative humidity compared with Al-Rowda. At high humidity people tend to modify the air temperature of their homes by using ventilators and air conditioning. Indoor bacterial frequency is usually associated with air conditioning filters that facilitate the growth of specific microorganisms . This might be due to the relative humidity, air quality and geographical characteristics. There are other contributing factors that were ascribed to the presence of specific bacteria including, temperature, ventilation strategy, rainfall and building design . Bowers et al.  reported that the diversity and composition of the airborne bacterial communities varied across sites and over time. Our data suggest the contribution of composite factors in defining the potential microbial community dynamics including weather conditions and the available microbial sources. Aydogdu et al.  and Muhammad et al.  noticed that poor ventilation, crowded conditions and increase in the number of air conditioning units inside buildings could facilitate the dispersal and the survival rates of airborne microorganisms that may intensify the probability of air borne infection. Previous studies reported that the occupants’ socioeconomic status, together with the level of hygienic practices may have significant impacts on air pathogens [21, 22, 41].
Monomorium ants, therefore, are a dangerous vector contributing to the spread of disease-causing bacteria. Concern must be focused on food storage hygiene, which is regarded as the main source of uncontrollable re-infestation. Homeowners must consider adopting preventive pest management through licensed professionals.
There were some difficulties at the beginning of ant collecting due to their fast movement. Collecting the ante by brushing or direct touching was not allowed at that stage in order to attain reliable results. This was solved by doing several trials before collecting the project sample.
JA conceived analyzed and interpreted the patient data regarding and was a major contributor in writing the final manuscript funding. QA contributed in collecting the ants. SL supervised and check the final version of manuscript. All authors read and approved the final manuscript.
My gratitude goes to NUERS—National Unit for Environmental Research and Services (SRUL-01/13) Project No. SRUL-01/13 for performing the analysis.
The authors declare that they have no competing interests.
Availability of data and materials
All data generated or analysed during this study are included in this published article.
Consent for publication
Ethics approval and consent to participate
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- Sarwar M. Insect vectors involving in mechanical transmission of human pathogens for serious diseases. Int J Bioinform Biomed Eng. 2015;1:300–6.Google Scholar
- Gore JC, Schal C. Cockroach allergen biology and mitigation in the indoor environment. Annu Rev Entomol. 2007;52:439–63.View ArticleGoogle Scholar
- Gore JC, Schal C. Cockroach allergen biology and mitigation in the indoor environment germanica. Med Vet Entomol. 2005;19:127–34.View ArticleGoogle Scholar
- Santos PF, Fonseca AR, Sanches NM. Formigas (Hymenoptera: Formicidae) como vetores de bactérias em dois hospitais do município de Divinópolis, Estado de Minas Gerais. Rev Soc Bras Med Trop. 2009;42:565–9.View ArticleGoogle Scholar
- Zarzuela MFM, Ribeiro MCC, Campos-Farinha AEC. Distribuição de formigas urbanas em um hospital da Região Sudeste do Brasil. Arq Inst Biol. 2002;69:85–7.Google Scholar
- Bulet P, Hetru C, Dimarcq JL, Hoffmann D. Antimicrobial peptides in insects; structure and function. Dev Comp Immunol. 1999;23:329–44.View ArticleGoogle Scholar
- Alsharani M, Alanazi M, Alsalamah M. Black ant stings caused by Pachycondyla sennaarensis: a significant health hazard. Ann Saudi Med. 2009;29:207–11.View ArticleGoogle Scholar
- Whitmore R, Kelly J, Reading P. National home and garden pesticide use survey, final report, volume I: executive summary, results, and recommendations. Research Triangle Park, NC: Research Triangle Institute/U.S. EPA; 1992.Google Scholar
- Whitemore RW, Immerman FW, Camann DE, Bond AE, Lewis RG, Schaum JL. Non-occupational exposures to pesticides for residents of two U.S. cities. Arch Environ Contam Toxicol. 1994;26:47–59.View ArticleGoogle Scholar
- Pereira R, Stimac J. Biocontrol options for urban pest ants. J Agric Entomol. 1997;14:231–48.Google Scholar
- Silva NC, Mirian MP, Pesquero M, Lilian C. Assessment of ants as bacterial vector in houses. Afr J Microbiol Res. 2014;8:1413–8.View ArticleGoogle Scholar
- Martins J, Martins A, Milagres R, Andrade N. Resistência a antibióticos de Staphylococcus aureus isolados de dietas enterais em um hospital público de Minas Gerais. Cienc Biol Saúde. 2007;28:9–14.View ArticleGoogle Scholar
- Hota B. Contamination, disinfection, and cross-colonization: are hospital surfaces reservoirs for nosocomial infection? Clin Infect Dis. 2004;39:1182–9.View ArticleGoogle Scholar
- Lima WR, Marques SG, Rodrigues FS, Rebelo JM. Ants in a hospital environment and their potential as mechanical bacterial vectors. Rev Soc Bras Med Trop. 2013;46:637–40.View ArticleGoogle Scholar
- Oliveira FMP, Ribeiro-Neto JD, Andersen AN, Leal IR. Chronic anthropogenic disturbance as a secondary driver of ant community structure: interactions with soil type in Brazilian Caatinga. Environ Conserv. 2016;44:115–23.View ArticleGoogle Scholar
- Moreira D, Morais VD, Vieira-da-Motta O, Campos-Farinha AEDC, Tonhasca A Jr. Ants as carriers of antibiotic-resistant bacteria in hospitals. Neotrop Entomol. 2005;34:999–1006.View ArticleGoogle Scholar
- Máximo H, Felizatti H, Ceccato M, Cintra-Socolowski P, Beretta A. Ants as vectors of pathogenic microorganisms in a hospital in São Paulo county, Brazil. BMC Res Notes. 2014;7:554.View ArticleGoogle Scholar
- Fowler HG, Bueno OC, Sadatsune T, Montelli AC. Ants as potential vectors of pathogens in hospitals in the state of Sao Paulo, Brazil. Insect Sci Appl. 1993;14:367–70.Google Scholar
- Welker C, Both J, Longaray S, Haas S, Soeiro M, Ramos R. Análise microbiológica dos alimentos envolvidos em surtos de Doenças Transmitidas Por Alimentos (DTA) ocorridos no estado do Rio Grande do Sul, Brasil. Rev Bras Biosci. 2010;8:44–8.Google Scholar
- Muhammad MH, Uba F, Madinat R, Muhd M, Sulaiman MA, Zubair MS. Isolation and identification of airborne bacteria from federal university dutse lecture rooms. Int J Sci Technol Res. 2017;6:16–9.Google Scholar
- Fang Z, Ouyang Z, Zheng H, Wang X, Hu L. Culturable airborne bacteria in outdoor environments in Beijing, China. Microb Ecol. 2007;54:487–96.View ArticleGoogle Scholar
- Hospodsky D, Qian J, Nazaroff WW, Yamamoto N, Bibby K, Rismani-Yazdi H, et al. Human occupancy as a source of indoor airborne bacteria. PLoS ONE. 2012;7:e34867.View ArticleGoogle Scholar
- Anas G, Aligbe DS, Suleiman G, Warodi FA. Studies on microorganisms associated with air-conditioned environments. IOSR J Environ Sci Toxicol Food Technol. 2016;10:16–8.Google Scholar
- Nordel EA. American review of respiratory diseases 2000; 501–503.Google Scholar
- Alteri CJ, Mobley HL. Escherichia coli physiology and metabolism dictates adaptation to diverse host microenvironments. Curr Opin Microbiol. 2012;15:3–9.View ArticleGoogle Scholar
- Ejrnaes K. Bacterial characteristics of importance for recurrent urinary tract infections caused by Escherichia coli. Dan Med Bull. 2011;58:B4187.PubMedGoogle Scholar
- Jantsch J, Chikkaballi D, Hensel M. Cellular aspects of immunity to intracellular Salmonella enterica. Immunol Rev. 2011;240:185–95.View ArticleGoogle Scholar
- Holmes B, Costas M, Ganner M, On SL, Stevens M. Evaluation of biolog system for identification of some gram-negative bacteria of clinical importance. J Clin Microbiol. 1994;32:1970–5.PubMedPubMed CentralGoogle Scholar
- Pawlowski SW, Warren CA, Guerrant R. Diagnosis and treatment of acute or persistent diarrhea. Gastroenterology. 2009;136:1874–86.View ArticleGoogle Scholar
- Pandey R, Mishra A. Antibacterial activities of crude extract of Aloe barbadensis to clinically isolated bacterial pathogens. Appl Biochem Biotechnol. 2010;160:1356–61.View ArticleGoogle Scholar
- Zumla A, Hui D, Al-Tawfiq J, Gautret P, McCloskey B, Memish Z. Emerging respiratory tract infections. Lancet Infect Dis. 2014;14:910–1.View ArticleGoogle Scholar
- Nevalainen A, Seuri M. Of microbes and men. Indoor Air. 2005;15(Suppl 9):58–64.View ArticleGoogle Scholar
- Kim KY, Kim CN. Airborne microbiological characteristics in public buildings of Korea. Build Environ. 2007;42:2188–96.View ArticleGoogle Scholar
- Balasubramanian D, Ohneck EA, Chapman J, et al. Staphylococcus aureus coordinates leukocidin expression and pathogenesis by sensing metabolic fluxes via RpiRc. MBio. 2016;7:e00818.View ArticleGoogle Scholar
- Regalado NG, Martin G, Antony SJ. Acinetobacter lwoffii: bacteremia associated with acute gastroenteritis. Travel Med Infect Dis. 2009;7(5):316–7.View ArticleGoogle Scholar
- Rathinavelu S, Zavros Y, Merchant JL. Acinetobacter lwoffii infection and gastritis. Microbes Infect. 2003;5(7):651–7.View ArticleGoogle Scholar
- Tas MY, Oguz MM, Ceri M. Acinetobacter lwoffii peritonitis in a patient on automated peritoneal dialysis: a case report and review of the literature. Case Rep Nephrol. 2017;2017:5760254.PubMedPubMed CentralGoogle Scholar
- Meadow JF, Altrichter AE, Bateman AC, Stenson J, Brown GZ, Green JL, et al. Humans differ in their personal microbial cloud. PeerJ. 2015;3:e1258.View ArticleGoogle Scholar
- Bowers RM, Clements N, Emerson JB, Wiedinmyer C, Hannigan MP, Fierer N. Seasonal variability in bacterial and fungal diversity of the near-surface atmosphere. Environ Sci Technol. 2013;47:12097–106.View ArticleGoogle Scholar
- Aydogdu H, Asan A, Tatman M. Indoor and outdoor airborne bacteria in child day care centres in Edirne city (Turkey) seasonal distribution and influence of meteorological factors. Environ Monit Assess. 2010;164:53–66.View ArticleGoogle Scholar
- Kembel SW, Meadow JF, O’Connor TK, Mhuireach G, Northcutt D, Kline J, et al. Architectural design drives the biogeography of indoor bacterial communities. PLoS ONE. 2014;9:e87093.View ArticleGoogle Scholar