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Correction to: A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro

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The original article was published in BMC Research Notes 2019 12:242

Correction to: BMC Res Notes (2019) 12:242

After publication of the original article [1], the authors became aware of a miscalculation in the original Fig. 2d.

$$ \frac{{\% \,{\text{HIV-}}1^{ + \,} {\text{activated}}\,{\text{cells}}\,{\text{at}}\,{\text{Day}}\,5 - \% \,{\text{HIV-}}1^{ + \,} \,{\text{resting}}\,{\text{cells}}\,{\text{at}}\,{\text{Day}}\,5}}{{\% \,{\text{HIV-}}1^{ + \,} {\text{activated}}\,{\text{cells}}\,{\text{at}}\,{\text{Day}}\,5}} \times 100 $$

should be calculated as:

$$ \frac{{\% \,{\text{HIV-}}1^{ + \,} {\text{activated}}\,{\text{cells}}\,{\text{at}}\,{\text{Day}}\,5 - \% \,{\text{HIV-}}1^{ + \,} \,{\text{resting}}\,{\text{cells}}\,{\text{at}}\,{\text{Day}}\,5}}{{\% \,{\text{HIV-}}1^{ + \,} \,{\text{resting}}\,{\text{cells}}\,{\text{at}}\,{\text{Day}}\,5}} \times 100 $$

The corrected Fig. 2d is shown in this erratum.

Fig. 2

HIV-1 infection and culture of resting CD4+ T-cell subsets isolated by cell sorting. Subsets of naïve T cells (TN), or CCR5+ or CCR5 memory T cells (TM), were separately infected and cultured. a Schematic of the protocol of HIV-1 infection and culture. b Representative flow-cytometry profiles of cells from Donor #1 at day 3 and day 5 post-infection (resting or activated), separated according to reporter expression indicating the presence of X4 or R5 HIV-1, with the percentage of each subset indicated (left panels). The intensity of fluorescence for each viral reporter in each cell subset [except for the very low percentage of DsRed+ cells (R5+) in TN cells] is shown in the right-hand panels. c Percentages of HIV-1+ cells in each CD4+ T-cell subset in three donors. d Percentage increases in frequencies of HIV-1+ cells following activation were estimated by comparing percentages of HIV-1+ cells in the activation condition with those in the resting condition at day 5 post-infection. Significant differences (*P < 0.05, **P < 0.01) were determined by repeated-measures one-way ANOVA followed by Tukey’s multiple comparison test. In c and d, HIV-1+ cells include the corresponding reporter (either EGFP or DsRed) single-positive cells and double-positive cells

Although the statistical significances have been altered, the hierarchical mode between cell-subset groups remains the same. It is still shown that numbers of X4 HIV-1+ cells increased consistently in the CCR5+ TM subset of all three donors tested. Therefore, the correction does not change the scientific conclusion.


  1. 1.

    Terahara K, Iwabuchi R, Hosokawa M, Nishikawa Y, Takeyama H, Takahashi Y, Tsunetsugu-Yokota Y. A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro. BMC Res Notes. 2019;12:242.

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Correspondence to Kazutaka Terahara.

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