Fig. 3
From: Dynamics of actin polymerisation during the mammalian single-cell wound healing response
RhoA activity is increased the over entire area of the wound-site. a Maximum intensity projections of selected micrographs of the CellMask, UtrCH, and γGBD signals following laser-mediated ablation of the PM of HeLa cells. b Normalized γGBD fluorescent signal intensity (γGBD FIR) at the wound site relative to control regions. Mean and SEM shown of n = 12 cells. c Kymographs of the UtrCH and γGBD signals before and after laser-mediated ablation of the PM. Cell and targeted area is the same as in the one shown in a. Targeted area (95 μm2) is represented by the red circle found on the pre-wounded, PM micrograph. The line scan region used for the creation of the kymograph correspond to the cyan line (22 μm) displayed on the 0 s, γGBD micrograph. The cell shown in a, c is representative of n = 12 wounding assays, all of which were used to prepare b. Scale bars = 10 μm